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ID 52138
JaLCDOI
FullText URL
Thumnail 68_1_7.pdf 9.39 MB
Author
Horimoto, Naoya
Kitamura, Shinji Kaken ID
Tsuji, Kenji
Abstract
Immunosuppressive agents are generally administered to treat kidney diseases. However, it is unclear whether renal stem/progenitor cells are directly affected by the immunosuppressive agents. We used normal rat kidney cells, ureteric bud cells and rat kidney stem/progenitor cells in this study. Mizoribine (MZR), cyclophosphamide (CPA) and cyclosporine (CyA) were added to the culture media of these cells. We evaluated the effects of these immunosuppressive agents on cell proliferation using an electrical cell-substrate impedance sensing system (ECIS) and their effects on the process of renal regeneration using the ischemia-reperfusion (I/R) injury rat model. The ECIS data showed that proliferation of each of the 3 types of cells was significantly suppressed by MZR. MZR treatment enhanced renal tubular injury in ischemia-reperfusion (I/R) injured rats, and significantly decreased levels of M-phase cells and Nestin-positive cells. These results suggested that MZR inhibits the cell cycle of renal stem/progenitor cells;thus, physicians should take note that MZR might affect not only inflammation but also renal regeneration.
Keywords
cell biology
immunosuppression
stem cells
Amo Type
Original Article
Published Date
2014-02
Publication Title
Acta Medica Okayama
Volume
volume68
Issue
issue1
Publisher
Okayama University Medical School
Start Page
7
End Page
15
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
Copyright Holders
CopyrightⒸ 2014 by Okayama University Medical School
File Version
publisher
Refereed
True
PubMed ID
Web of Science KeyUT
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/52247