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ID 20411
Eprint ID
20411
FullText URL
Title Alternative
Evaluation of genotoxic potential in sewage and treated effluent with the umu-test
Author
Mohri, Shino
Ono, Yoshiro
Abstract
The umu-test, which can detect genotoxic activites of a wide variety of environmental carcinogens and mutagenes, was carried out with samples from several sewage treatment processes. We applied the umu-test to raw sewage and treated effluent from two points of view, one is to evaluate a treatment process and the other is to identify the genotoxic substances. The original strain of umu-test, Salmonella typhimurium TA1535/pSK1002 can detect nonspecific genotoxicity, on the other hand, by using S. typhimurium NM2009 of the umu-test highly sensitive to aromatic amines, genotoxicity related to aromatic amines is measured. The result showed that genotoxic substances which have the polycyclic aromatic amino structures in the sewage remained after biological treatment. Tow-step HPLC fractionation followed by MS analysis to identify the genotoxic substances was applied to samples to detect mutagenic and carcinogenic heterocyclic amines. The concentration of Trp-P-1 and Trp-P-2 determined in the effluent caluculated from HPLC-UV analysis were on the order of 10(-5) mg/L. Because of the possibility of the reuse of wastewater in urban areas, the need for the investigation of the safety of the treated water from the sewage treatment plants was suggested, in order to evaluate strategies for advanced treatment, if necessary.
Note
論文
Published Date
2000-09
Publication Title
環境制御
Publication Title Alternative
Environmental Research and Control
Volume
volume22
Publisher
岡山大学環境管理センター
Publisher Alternative
Okayama University Administration Center for Environmental Science and Technology
Start Page
26
End Page
31
ISSN
0917-1533
NCID
AN1024789X
Content Type
Departmental Bulletin Paper
language
日本語
File Version
publisher
Refereed
True
Eprints Journal Name
erc