Author Tamura, Takashi| Tsunekawa, Naoki| Nemoto, Michiko| Inagaki, Kenji| Hirano, Toshiyuki| Sato, Fumitoshi|
Published Date 2016-01-28
Publication Title Scientific reports
Volume volume6
Content Type Journal Article
Title Alternative Recombinant expression and characterization of quinone-containing novel glycine oxidase from Marinomonas mediterranea
FullText URL srfa_109_001_006.pdf
Author Kajiyama, Yuki| Mizobata, Satsuki| Akaji, Shusaku| Nemoto, Michiko| Tamura, Takashi| Inagaki, Kenji|
Abstract  Novel glycine oxidase (GlyOX) from Marinomonas mediterranea depends on cysteine tryptophilquinone (CTQ) and catalyzes the oxidative deamination of glycine to produce a glyoxylate, ammonia, and hydrogen peroxide. M. mediterranea GlyOX genes (goxA and goxB) were cloned and recombinant GlyOX was heterologously expressed by E. coli. The purification of recombinant GlyOX was carried out by metal affinity and DEAE-Toyopearl 650M column chromatographies. M. mediterranea GlyOX was homotetramic with a molecular mass of 76kDa and showed optimum activity around 30°C and at pH 5.0, and stability below 50°C and between pH 5.0 to 9.0. M. mediterranea GlyOX shows a strict substrate specificity toward glycine, and the Michaelis constant for glycine was 0.5mM. M. mediterranea GlyOX could determine the quantity of glycine in human serum and human blood plasma with high sensitivity. This study revealed the catalytic and structural properties of M. mediterranea GlyOX with high substrate specificity.
Abstract Alternative , , ,
Keywords glycine oxidase Marinomonas mediterranea cysteine tryptophilquinone recombinant expression enzymatic glycine assay
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 1
End Page 6
ISSN 2186-7755
language 日本語
File Version publisher
Author Tamura, Takashi| Li, Yin Shu| Tanaka, Hidehiko| Inagaki, Kenji|
Published Date 2002-02
Publication Title 岡山大学農学部学術報告
Volume volume91
Issue issue1
Content Type Departmental Bulletin Paper
Author Oshima, Kenshiro| Hattori, Masahira| Shimizu, Hitomi| Fukuda, Koji| Nemoto, Michiko| Inagaki, Kenji| Tamura, Takashi|
Published Date 2015-07-09
Publication Title Genome Announcements
Volume volume3
Issue issue4
Content Type Journal Article
Author Nemoto, Michiko| Iwaki, Sayako| Moriya, Hisao| Monden, Yuki| Tamura, Takashi| Inagaki, Kenji| Mayama, Shigeki| Obuse, Kiori|
Keywords Biomineralization Diatom Silica Transcriptome Proteome
Note This is a post-peer-review, pre-copyedit version of an article published in Marine Biotechnology. The final authenticated version is available online at: </br>This fulltext is available in June 2021.|
Published Date 2020-06-03
Publication Title Marine Biotechnology
Publisher Springer
ISSN 1436-2228
NCID AA11357643
Content Type Journal Article
language フランス語
OAI-PMH Set 岡山大学
File Version author
PubMed ID 32488507
DOI 10.1007/s10126-020-09976-1
Web of Science KeyUT 000537432000001
Related Url isVersionOf
Author Seow, Teck Keong| Inagaki, Kenji| Tamura, Takashi| Tanaka, Hidehiko|
Published Date 1997-02
Publication Title 岡山大学農学部学術報告
Volume volume86
Issue issue1
Content Type Departmental Bulletin Paper