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ID 52037
FullText URL
Author
Shinaoka, Akira
Shiratsuchi, Eri
Kumagishi, Kanae
Nakahara, Ryuichi
Naito, Ichiro
Abstract
Most blood vessels contain elastin that provides the vessels with the resilience and flexibility necessary to control hemodynamics. Pathophysiological hemodynamic changes affect the remodeling of elastic components, but little is known about their structural properties. The present study was designed to elucidate, in detail, the three-dimensional (3D) architecture of delicate elastic fibers in small vessels, and to reveal their architectural pattern in a rat model. The fine vascular elastic components were observed by a newly developed scanning electron microscopy technique using a formic acid digestion with vascular casts. This method successfully visualized the 3D architecture of elastic fibers in small blood vessels, even arterioles and venules. The subendothelial elastic fibers in such small vessels assemble into a sheet of meshwork running longitudinally, while larger vessels have a higher density of mesh and thicker mesh fibers. The quantitative analysis revealed that arterioles had a wider range of mesh density than venules; the ratio of density to vessel size was higher than that in venules. The new method was useful for evaluating the subendothelial elastic fibers of small vessels and for demonstrating differences in the architecture of different types of vessels.
Keywords
vascular corrosion casting
elastic fiber
elastin
SEM
formic acid digestion
rat
three-dimensional architecture
Published Date
2013-04
Publication Title
Microscopy and Microanalysis
Volume
volume19
Issue
issue2
Start Page
406
End Page
414
ISSN
1431-9276
Content Type
Journal Article
Official Url
http://dx.doi.org/10.1017/S1431927612014341
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/51967
language
英語
File Version
publisher
Refereed
True
DOI
Web of Sience KeyUT