JaLCDOI 10.18926/AMO/54416
FullText URL 70_3_175.pdf
Author Setiawan, Heri| Nagaoka, Kenjiro| Kubo, Masayuki| Fujikura, Yoshihisa| Ogino, Keiki|
Abstract Oxidative stress is widely known to play a role in asthma. However, the contribution of xanthine oxidoreductase (XOR) as a source of the superoxide anion radical (O2-) in oxidative stress associated with asthma has not yet been examined in detail. Here we investigated pathophysiological changes in XOR in an experimental model of asthma induced by the house dust mite Dermatophagoides farinae (Df). In the lungs of Df-treated mice, the production of O2- from XOR increased and the nitrite concentrations decreased, whereas the protein expression of XOR remained unchanged. Moreover, the protein expression levels of XOR and the hydrogen peroxide (H2O2) concentrations in bronchoalveolar lavage fluid (BALF) were higher in the Df-treated mice than in saline-treated mice. Immunohistochemically, although XOR was highly localized in the bronchial epithelial cells of the saline-treated mice, immunostaining for XOR was absent in the bronchial epithelium of Df-treated mice. These results suggest that oxidative stress is up-regulated by increases in the conversion of the dehydrogenase form (xanthine dehydrogenase; XDH) of XOR to the oxidase form (xanthine oxidase; XOD).
Keywords xanthine oxidase oxidative stress asthma
Amo Type Original Article
Published Date 2016-06
Publication Title Acta Medica Okayama
Volume volume70
Issue issue3
Publisher Okayama University Medical School
Start Page 175
End Page 182
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2016 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 27339206
Web of Sience KeyUT 000379406100004
Author Zou, Yu| Wang, Da-Hong| Sakano, Noriko| Sato, Yoshie| Iwanaga, Suketaka| Taketa, Kazuhisa| Kubo, Masayuki| Takemoto, Kei| Masatomi, Chie| Inoue, Kiyomi| Ogino, Keiki|
Published Date 2014-02
Publication Title International Journal of Environmental Research and Public Health
Volume volume11
Issue issue2
Content Type Journal Article
JaLCDOI 10.18926/AMO/52652
FullText URL 68_3_129.pdf
Author Minoura, Akira| Wang, Da-Hong| Sato, Yoshie| Zou, Yu| Sakano, Noriko| Kubo, Masayuki| Takemoto, Kei| Masatomi, Chie| Ogino, Keiki|
Abstract We examined the relationships between dietary carbohydrate, protein, fat, and the ratio of n6/n3 fatty acid intakes with the predicted 10-year coronary heart disease (CHD) risk in a general Japanese population. We used the Framingham risk score to determine the 10-year CHD risk of the subjects, who were employees of 6 companies in a single prefecture in Japan. After excluding the subjects who reported any history of angina pectoris, myocardial infarction, diabetes, or cancer, and those with missing data resulting in the inability of estimation of 10-year CHD risk and food intakes, the final data analysis was carried out for 809 subjects. The logistic regression models revealed a significantly increased odds ratio of 10-year CHD risk in the subjects with the highest tertile of carbohydrate intake (% energy) (odds ratio 3.64, 95% CI, 2.07-6.40);after adjustment for other variables, the odds ratio for the 10-year CHD risk was also higher in the subjects with the highest tertile of carbohydrate intake (odds ratio 1.72, 95% CI, 0.70-4.25). We also found that fat intake and the ratio of n6/n3 fatty acids were inversely associated with the predicted 10-year CHD risk (p for trend<0.01). The present findings added evidence of a positive association of dietary carbohydrate and inverse associations of total fat and n6/n3 fatty acid ratio with the predicted 10-year CHD risk in a general Japanese population.
Keywords fat intake carbohydrate intake ratio of n6/n3 fatty acids 10-year coronary heart disease risk Japanese
Amo Type Original Article
Published Date 2014-06
Publication Title Acta Medica Okayama
Volume volume68
Issue issue3
Publisher Okayama University Medical School
Start Page 129
End Page 135
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2014 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 24942790
Web of Sience KeyUT 000337655600001
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/52516
Author Iwanaga, Suketaka| Sakano, Noriko| Taketa, Kazuhisa| Takahashi, Noriko| Wang, Da-Hong| Takahashi, Hidekazu| Kubo, Masayuki| Miyatake, Nobuyuki| Ogino, Keiki|
Published Date 2013-02-01
Publication Title Obesity Research & Clinical Practice
Content Type Journal Article
Author Inoue, Kiyomi| Sakano, Noriko| Ogino, Keiki| Sato, Yoshie| Wang, Da-Hong| Kubo, Masayuki| Takahashi, Hidekazu| Kanbara, Sakiko| Miyatake, Nobuyuki|
Published Date 2013-03-01
Publication Title Journal of Clinical Biochemistry and Nutrition
Volume volume52
Issue issue2
Content Type Journal Article
Author Takiue, Keiichi| Sugiyama, Hitoshi| Inoue, Tatsuyuki| Morinaga, Hiroshi| Kikumoto, Yoko| Kitagawa, Masashi| Kitamura, Shinji| Maeshima, Yohei| Wang, Dahong| Masuoka, Noriyoshi| Ogino, Keiki| Makino, Hirofumi|
Published Date 2012-05-25
Publication Title BMC Nephrology
Volume volume13
Issue issue14
Content Type Journal Article
JaLCDOI 10.18926/AMO/48561
FullText URL 66_3_221.pdf
Author Takahata, Yoko| Wang, Da-Hong| Anai, Takanobu| Ogino, Keiki|
Abstract This study aimed to elucidate the relationship of prenatal and/or postnatal factors, including acquired factors, with the calcaneus stiffness index as measured by quantitative ultrasound (QUS-SI) in adolescents. We recruited 1,143 adolescents with a mean age of 14.8±1.8 years (501 boys and 642 girls). The subjectsʼ calcaneus QUS-SI was measured using an ultrasound bone densitometer. We also measured the subjectsʼ height, weight, and grip strength. Data on prenatal and postnatal factors were obtained from maternal and child health handbooks. A self-reporting questionnaire was used to obtain information on subjectsʼ secondary sexual characteristics and lifestyle factors. We found that maternal weight gain during pregnancy was independently associated with calcaneus QUS-SI in girls, and that grip strength was also significantly associated with calcaneus QUS-SI in both sexes. The present findings suggest that excessive restriction of maternal weight gain would have a negative effect on the calcaneus QUS-SI of girls, and that exercise and strength-building activities are likely to result in a higher calcaneus QUS-SI in both sexes of adolescents.
Keywords adolescents calcaneus QUS-SI prenatal and/or postnatal status stiffness index ultrasound bone densitometer
Amo Type Original Article
Published Date 2012-06
Publication Title Acta Medica Okayama
Volume volume66
Issue issue3
Publisher Okayama University Medical School
Start Page 221
End Page 229
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2012 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 22729102
Web of Sience KeyUT 000305669700005
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/48455
JaLCDOI 10.18926/AMO/43829
FullText URL 65_1_41.pdf
Author Tokumori, Kimihiko| Wang, Da-Hong| Takigawa, Tomoko| Takaki, Jiro| Ogino, Keiki|
Abstract This study aimed to determine whether there was any association between the regional climate and the proportion of people with joint pain. Regional climate data between 1971 and 2000 were obtained from the Japan Meteorological Agency. The variables used in the cluster analysis included sunlight hours, amount of precipitation, number of days with precipitation, and temperature. The regional proportion of people with joint pain was obtained from the National Survey for Health in 2001. After performing a cluster analysis, one-way ANOVA and Welch's test were used to determine whether the climate characteristics of the clusters were significantly different. Within each cluster, stepwise multiple linear regression analyses were performed. We found that sunlight hours showed a direct, negative association with the proportion of people with joint pain (adjusted R2=0.532, p=0.016) in cluster 1, which was characterized as the region with the fewest total hours of sunlight, less precipitation, a modest number of rainy days, and low temperature. In the other clusters, the regional female population rate (cluster 2) and the senior population rate (cluster 3, 4) were the primary predictors. We concluded that the degree of exposure to sunlight may play a crucial role in prevention of joint pain. This finding should encourage people to set aside some time for staying outdoors in their daily lives.
Keywords climate joint pain sunlight hours cluster analysis
Amo Type Original Article
Published Date 2011-02
Publication Title Acta Medica Okayama
Volume volume65
Issue issue1
Publisher Okayama University Medical School
Start Page 41
End Page 48
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2011 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 21339795
Web of Sience KeyUT 000287620500006
JaLCDOI 10.18926/AMO/32911
FullText URL fulltext.pdf
Author Takemoto, Kei| Ogino, Keiki| Wang, Da-Hong| Takigawa, Tomoko| Kurosawa, Carmen M.| Kambayashi, Yasuhiro| Hibino, Yuri| Hitomi, Yoshiaki| Ichimura, Hiroshi|
Abstract <p>It is well known that eosinophils are involved in tyrosine nitration. In this study, we evaluated tyrosine nitration by rat eosinophils isolated from peritoneal fl uid and constituent eosinophils in the stomach. Rat peritoneal eosinophils activated with 1 &#956;M phorbol myristate acetate (PMA) and 50 &#956;M NO2 &#65437; showed immunostaining for nitrotyrosine only in smaller cells, despite the fact that eosinophils are capable of producing superoxide (O2·&#65437;). Free tyrosine nitrating capacity after incubation with PMA and NO2 &#65437; was 4-fold higher in eosinophils than in neutrophils. Catalase and &#65400;- and &#65402; -tocopherol inhibited free tyrosine nitration by reactive nitrogen species from eosinophils but not that by peroxynitrite. Superoxide dismutase augmented free tyrosine nitration by activated eosinophils and peroxynitrite. The concentration of nitric oxide released from eosinophils was relatively low (0.32 &#956;M/106 cells/h) and did not contribute to the formation of nitrotyrosine. On the other hand, most constituent eosinophils constituent in the rat stomach stimulated by PMA and NO2 &#65437; showed tyrosine nitration capacity. These results suggest that intact cells other than apoptotic-like eosinophils eluted in the intraperitoneal cavity could not generate reactive species responsible for nitration by a peroxidase-dependent mechanism. In contrast, normal eosinophils in the stomach were capable of nitration, suggesting that the characteristics of eosinophils in gastric mucosa are diff erent from those eluted in the peritoneal cavity.</p>
Keywords eosinophil peroxidase reactive nitrogen species nitrotyrosine
Amo Type Article
Published Date 2007-02
Publication Title Acta Medica Okayama
Volume volume61
Issue issue1
Publisher Okayama University Medical School
Start Page 17
End Page 30
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 17332838
Web of Sience KeyUT 000244432400003
JaLCDOI 10.18926/AMO/32887
FullText URL fulltext.pdf
Author Yajima, Yuki| Tsutsui, Takako| Nakajima, Kazuo| Li, Hui-Ying| Takigawa, Tomoko| Wang, Da-Hong| Ogino, Keiki|
Abstract <p>The purpose of the study was to investigate the over-time effects of physical, psychological and social resources on the incidence of depression in family caregivers of the disabled elderly. Data were collected twice at a one-year interval from 1,141 primary caregivers of a disabled older person in an urban area of Japan using a self-reported questionnaire survey. The questionnaire included physical health as an indicator of physical resources, caregiving satisfaction and intention to care as indicators of psychological resources, and instrumental and emotional support network and formal home care service utilization as indicators of social resources. The mental health outcome measure was the General Health Questionnaire 12-item version (GHQ-12). Complete data on 235 non-depressed female caregivers were separated into 3 groups according to the relationship type (wife, daughter and daughter-in-law) and analyzed separately. Multivariate logistic regression models controlling for duration of caregiving, care-recipient's gender, ADL dependency and behavioral problems demonstrated that significant predictors of depression were caregiving satisfaction and intention to care in wives, caregiving satisfaction in daughters, and physical health and emotional support network in daughters-in-law. Noteworthy, intention to care increased the risk of depression in wives, while decreasing the risk of depression in daughters-in-law. The findings indicate that the effects of caregivers' resources on mental health may differ by relationship type.</p>
Keywords caregivers kin relationships caregiving resources depression
Amo Type Original Article
Published Date 2007-04
Publication Title Acta Medica Okayama
Volume volume61
Issue issue2
Publisher Okayama University Medical School
Start Page 71
End Page 80
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 17471307
Web of Sience KeyUT 000245875600004
JaLCDOI 10.18926/AMO/32871
FullText URL fulltext.pdf
Author Ogino, Keiki| Wang, Da-Hong|
Abstract Oxidative/nitrosative stress is responsible for a variety of degenerative processes in some human diseases. Measurement of oxidatively/nitrosatively modified DNA, proteins, lipids, and sugars in biological samples has been expected to detect appropriate biomarkers for diseases in which reactive oxygen/nitrogen species are involved. Recently, the application of these biomarkers to epidemiological studies has resulted in a new discipline, molecular epidemiology, which provides the opportunity for better understanding of their causal relation with disease outcomes in a population level. In this brief review, we cover some specific biomarkers of oxidative/nitrosative stress with regard to the commonly used analytical methods for these biomarkers, their integration with epidemiology, and their application in antioxidant intervention trials, with an emphasis on those applicable to human studies and their potentialities for disease prevention.
Keywords biomarker oxidative/nitrosative stress molecular epidemiology disease prevention
Amo Type Review
Published Date 2007-08
Publication Title Acta Medica Okayama
Volume volume61
Issue issue4
Publisher Okayama University Medical School
Start Page 181
End Page 189
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 17726507
Web of Sience KeyUT 000248957100001
JaLCDOI 10.18926/AMO/31833
FullText URL fulltext.pdf
Author Mori, Keiko| Suzuki, Hisao| Wang, Da Hong| Takaki, Jiro| Takigawa, Tomoko| Ogino, Keiki|
Abstract <p>The present study aimed to investigate the status of physical activity and the differences in psychological factors associated with physical activity from the perspective of transtheoretical model stages between prime- and middle-aged Japanese. The study involved 375 prime-aged volunteers (175 men, 200 women) and 557 middle-aged volunteers (247 men, 310 women) living in Kuse, a town in Okayama Prefecture, Japan. We found that the prime-aged men at the preparation stage had significantly higher self-efficacy scores than at the contemplation stage (p&#60;0.01). Middle-aged men had significantly higher self-efficacy scores at the contemplation stage than at the precontemplation stage (p&#60;0.001). Middle-aged women, meanwhile, had significantly higher self-efficacy scores at the maintenance stage than at the action stage (p&#60;0.01), and at the contemplation stage than at the precontemplation stage (p&#60;0.001). The present findings provide valuable information about the differences in psychological factors affecting physical activity between prime-aged and middle-aged community-dwelling Japanese. This information may be useful to health professionals as they develop effective community-based intervention programs for target populations.</p>
Keywords transtheoretical model prime-aged and middle-aged Japanese physical activity self-efficacy interest in physical activity for improving health
Amo Type Original Article
Published Date 2009-04
Publication Title Acta Medica Okayama
Volume volume63
Issue issue2
Publisher Okayama University Medical School
Start Page 97
End Page 104
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 19404341
Web of Sience KeyUT 000265457600004
JaLCDOI 10.18926/AMO/30970
FullText URL fulltext.pdf
Author Kurosawa, Carmen Miwa| Ito, Takehiko| Takaki, Jiro| Wang, Bing-Ling| Wang, Da-Hong| Takigawa, Tomoko| Ogino, Keiki|
Abstract <p>In the present study, we examined the dynamic of school-health-based parasite control and the related socio-economic influences. This is an ecological study based on data from 46 prefectures in Japan. The exponential decay of Ascaris lumbricoides prevalence was calculated by iterative least-squares method. Pearson&#700;s correlation and multiple linear regression model analysis were performed to assess the associations between the prevalence of Ascaris lumbricoides in Japanese school children and socio-economic variables such as the prefecture income per capita, the percentage of primary industry, the population density per 1 km2, the diffusion rate of population under water supply, and the percentage of upper secondary school enrollment. The results indicated that the parasite carrier rate was higher in younger students. The half-life of Ascaris lumbricoides prevalence was approximately 3 years with significant variation among prefectures. Multiple regression analyses showed that the decrease of infection in elementary and lower secondary school children had a significant positive association with primary industry and a significant negative association with prefecture income per capita. The school-health-based parasite intervention differs by prefecture and has changed over time according to the respective prefectural stage of economic development.</p>
Keywords Ascaris lumbricoides parasite control school-health-based approach economic growth
Amo Type Original Article
Published Date 2008-10
Publication Title Acta Medica Okayama
Volume volume62
Issue issue5
Publisher Okayama University Medical School
Start Page 303
End Page 312
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 18985090
Web of Sience KeyUT 000260391300004
JaLCDOI 10.18926/AMO/30957
FullText URL fulltext.pdf
Author Takemura, Yoko| Kishimoto, Takumi| Takigawa, Tomoko| Kojima, Shinji| Wanga, Bing-Ling| Sakano, Noriko| Wang, Da-Hong| Takaki, Jiro| Nishide, Tadashi| Ishikawa, Kou| Ogino, Keiki|
Abstract <p>To decrease the incidence of pneumoconiosis, we examined dust protective mask performance and its relation to pulmonary function as well as the effects of worker education on the proper wearing of masks. One hundred and seventy-eight workers from 15 factories subject to dust exposure participated in this study. All participants were interviewed to obtain relevant personal information and underwent both a mask leakage and a pulmonary function test. The mask leakage was expressed as a percentage, with under 10% leakage indicating that the dust protective mask worked efficiently. In addition, 23 workers from 2 factories were educated on how to wear masks properly. The average mask leakage was 24.3%, and 58% of workers wore ineffective masks. Though pulmonary function was almost normal, the percent vital capacity (%VC) tended to be lower depending on the mask leakage. Mask education, which was very easy and took only a short time, dramatically decreased average mask leakage from 32.1% to 10.5% (p0.001). Educating workers to wear masks properly might prevent the worsening of pulmonary function in response to dust exposure. Appropriate mask fitness by education could be useful in preventing the development of pneumoconiosis.</p>
Keywords pulmonary function education on proper wearing masks pneumoconiosis mask leakage occupational exposure
Amo Type Original Article
Published Date 2008-04
Publication Title Acta Medica Okayama
Volume volume62
Issue issue2
Publisher Okayama University Medical School
Start Page 75
End Page 82
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 18464883
Web of Sience KeyUT 000255297600003
JaLCDOI 10.18926/AMO/30951
FullText URL fulltext.pdf
Author Ogata, Masana| Wang, Da-Hong| Ogino, Keiki|
Abstract <p>The molecular defects in the catalase gene, levels of m-RNA and properties of the residual catalase studied by scientists are reviewed in human (Japanese, Swiss and Hungarian) and non-human (mouse and beagle dog) acatalasemia with reference to the bioinformatics. Japanese acatalasemia-I, the G to A transition at the fifth position of intron 4 of the catalase gene, limited the correct splicing of the mRNA and synthesized trace catalase with normal properties. Hungarian acatalasemia type C showed a splicing mutation. In the Japanese acatalasemia II and the type A and B of Hungarian acatalasemia, the deletion or insertion of nucleotides was observed in the coding regions, and the frame shift altered downstream amino acid sequences and formed truncated proteins. In the Hungarian acatalasemia D, the substitution of a nucleotide in the exon was found. In mouse and beagle dog acatalasemia, the substitution of nucleotides in the coding regions was also observed. Studies of residual catalase in Swiss, mouse and beagle dog acatalasemia showed that aberrant catalase protein degrades more quickly than normal catalase in cells. The experimental research in genetic toxicology concerning the effect of oxidative stressors (nitrogen monoxide, nitrogen dioxide and so on) on Japanese acatalasemic blood and acatalasemic mice is described. The clinical features of Japanese and Hungarian acatalasemic subjects are also described.</p>
Keywords acatalasemia catalase novel mutation bioinformatics genetic toxicology
Amo Type Review
Published Date 2008-12
Publication Title Acta Medica Okayama
Volume volume62
Issue issue6
Publisher Okayama University Medical School
Start Page 345
End Page 361
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
Web of Sience KeyUT 000262025000001
Author 荻野 景規|
Published Date 2007-01-04
Publication Title 岡山医学会雑誌
Volume volume118
Issue issue3
Content Type Journal Article
Author Kawakita, Norifumi| Miyatake, Nobuyuki| Takigawa, Tomoko| Wang, Da-Hong| Ogino, Keiki| Numata, Takeyuki|
Published Date 2007-09-03
Publication Title 岡山医学会雑誌
Volume volume119
Issue issue2
Content Type Journal Article