このエントリーをはてなブックマークに追加
ID 57820
FullText URL
Author
Suga, Michihiro Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID researchmap
Akita, Fusamichi Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID publons researchmap
Yamashita, Keitaro RIKEN SPring-8 Center
Nakajima, Yoshiki Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University ORCID
Ueno, Go RIKEN SPring-8 Center
Li, Hongjie Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University
Yamane, Takahiro Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University
Hirata, Kunio RIKEN SPring-8 Center
Umena, Yasufumi Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University Kaken ID publons researchmap
Yonekura, Shinichiro Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University ORCID
Yu, Long-Jiang Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University
Murakami, Hironori Japan Synchrotron Radiation Research Institute
Nomura, Takashi RIKEN SPring-8 Center
Kimura, Tetsunari Department of Chemistry, Graduate School of Science, Kobe University
Kubo, Minoru RIKEN SPring-8 Center
Baba, Seiki Japan Synchrotron Radiation Research Institute
Kumasaka, Takashi Japan Synchrotron Radiation Research Institute
Tono, Kensuke RIKEN SPring-8 Center
Yabashi, Makina RIKEN SPring-8 Center
Isobe, Hiroshi Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University
Yamaguchi, Kizashi The Institute for Scientific and Industrial Research, Osaka University
Yamamoto, Masaki RIKEN SPring-8 Center
Ago, Hideo RIKEN SPring-8 Center
Shen, Jian-Ren Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID publons researchmap
Abstract
Photosynthetic water oxidation is catalyzed by the Mn4CaO5 cluster of photosystem II (PSII) with linear progression through five S-state intermediates (S0 to S4). To reveal the mechanism of water oxidation, we analyzed structures of PSII in the S1, S2, and S3 states by x-ray free-electron laser serial crystallography. No insertion of water was found in S2, but flipping of D1 Glu189 upon transition to S3 leads to the opening of a water channel and provides a space for incorporation of an additional oxygen ligand, resulting in an open cubane Mn4CaO6 cluster with an oxyl/oxo bridge. Structural changes of PSII between the different S states reveal cooperative action of substrate water access, proton release, and dioxygen formation in photosynthetic water oxidation.
Note
This is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in [An oxyl/oxo mechanism for oxygen-oxygen coupling in PSII revealed by an x-ray free-electron laser] on 2019 Oct 18;366(6463), DOI: 10.1126/science.aax6998
Published Date
2019-10-18
Publication Title
Science
Volume
volume366
Issue
issue6463
Publisher
American Association for the Advancement of Science
Start Page
334
End Page
338
ISSN
0036-8075
NCID
AA00835277
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
File Version
author
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1126/science.aax6998