Author Yokoi, Tadashi| Senda, Masuo| Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Ashida, Kozo| Nishida, Norikazu| Tsugeno, Hirofumi| Okamoto, Makoto| Takata, Shingo| Tanizaki, Yoshiro| Inoue, Hajime|
Published Date 2002-02-01
Publication Title 岡大三朝分院研究報告
Volume volume72
Content Type Departmental Bulletin Paper
Author Yokoi, Tadashi| Senda, Masuo| Hamada, Masanori| Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Ashida, Kozo| Iwagaki, Naofumi| Nagata, Takuya| Fujii, Makoto| Takata, Shingo| Tanizaki, Yoshiro| Inoue, Hajime|
Published Date 2004-02-01
Publication Title 岡大三朝医療センター研究報告
Volume volume74
Content Type Departmental Bulletin Paper
Author Yokoi, Tadashi| Senda, Masuo| Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Ashida, Kozo| Nishida, Norikazu| Tsugeno, Hirofumi| Okamoto, Makoto| Nagata, Takuya| Takata, Shingo| Tanizaki, Yoshiro| Inoue, Hajime|
Published Date 2003-02-01
Publication Title 岡大三朝医療センター研究報告
Volume volume73
Content Type Departmental Bulletin Paper
JaLCDOI 10.18926/AMO/30512
FullText URL fulltext.pdf
Author Takata, Shingo| Yamamoto, Yuji| Ishizu, Hideo|
Abstract <p>A method of genotyping IgA2 alleles in the human immunoglobulin alpha 2 heavy chain constant region (C alpha 2 gene) was developed by using the polymerase chain reaction (PCR). By this method, the genotype was determined by discriminating base substitution in the 3'-flanking region of alleles, A2m*1 and A2m*2, which manifest A2m serum types, by nested PCR using allele-specific primers. Three types, IgA2*1/IgA2*1, IgA2*2/IgA2*1, and IgA2*2/IgA2*2, were detected from DNA extracted from lymphocytes. Genotyping was possible from 100 pg of DNA by this method. The estimated allele frequency in 318 Japanese subjects was 0.561 for IgA2*1 and 0.439 for IgA2*2. Analysis of 29 cases of paternity tests suggested that the data follow Mendel's law of inheritance. This genotype could also be detected in whole blood, blood stains, saliva stains, and various organs and tissues. These results suggest the usefulness of the present method for paternity testing and individual identification in forensic medicine.</p>
Keywords polymorphism deoxryibonucleic acid(DNA) immunoglobulin alpha 2 polymerase chain reaction(PCR) allele-specific amplificartion
Amo Type Article
Published Date 1996-02
Publication Title Acta Medica Okayama
Volume volume50
Issue issue1
Publisher Okayama University Medical School
Start Page 1
End Page 9
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8701775
Web of Science KeyUT A1996TY06000001
Author Hosaki, Yasuhiro| Takata, Shingo| Mitsunobu, Fumihiro| Mifune, Takashi| Ashida, Kozo| Tsugeno, Hirofumi| Okamoto, Makoto| Harada, Seishi| Tanizaki, Yoshiro| Ochi, Koji| Harada, Hideo| Tsuji, Takao|
Published Date 1998-12
Publication Title 岡大三朝分院研究報告
Volume volume69
Content Type Departmental Bulletin Paper
Author Mifune, Takashi| Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Ashida, Kozo| Tsugeno, Hirofumi| Harada, Seishi| Okamoto, Makoto| Yumoto, Eiichirou| Takata, Shingo| Tanizaki, Yoshiro|
Published Date 1998-12
Publication Title 岡大三朝分院研究報告
Volume volume69
Content Type Departmental Bulletin Paper
Author Mifune, Takashi| Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Ashida, Kozo| Tsugeno, Hirofumi| Harada, Seishi| Okamoto, Makoto| Yumoto, Eiichirou| Takata, Shingo| Tanizaki, Yoshiro|
Published Date 1998-12
Publication Title 岡大三朝分院研究報告
Volume volume69
Content Type Departmental Bulletin Paper
Author Okamoto, Makoto| Ashida, Kozo| Mitsunobu, Fumihiro| Mifune, Takashi| Hosaki, Yasuhiro| Tsugeno, Hirofumi| Harada, Seishi| Yumoto, Eiichiro| Takata, Shingo| Tanizaki, Yoshiro| Kataoka, Mikio| Harada, Mine|
Published Date 1998-12
Publication Title 岡大三朝分院研究報告
Volume volume69
Content Type Departmental Bulletin Paper
Author Harada, Seishi| Mitsunobu, Fumihiro| Kodama, Fumiko| Hosaki, Yasuhiro| Mifune, Takashi| Tsugeno, Hirofumi| Okamoto, Makoto| Yumoto, Eiichiro| Takata, Shingo| Tanizaki, Yoshiro|
Published Date 1998-12
Publication Title 岡大三朝分院研究報告
Volume volume69
Content Type Departmental Bulletin Paper
Author Mitsunobu, Fumihiro| Mifune, Takashi| Hosaki, Yasuhiro| Ashida, Kozo| Tsugeno, Hirofumi| Okamoto, Makoto| Harada, Seishi| Yumoto, Eiichiro| Takata, Shingo| Tanizaki, Yoshiro| Ochi, Koji| Harada, Hideo| Hasegawa, Harumi|
Published Date 1998-12
Publication Title 岡大三朝分院研究報告
Volume volume69
Content Type Departmental Bulletin Paper
Author Mitsunobu, Fumihiro| Mifune, Takashi| Hosaki, Yasuhiro| Ashida, Kozo| Tsugeno, Hirofumi| Okamoto, Makoto| Harada, Seishi| Yumoto, Eiichiro| Takata, Shingo| Ochi, Koji| Harada, Hideo| Hasegawa, Harumi|
Published Date 1998-12
Publication Title 岡大三朝分院研究報告
Volume volume69
Content Type Departmental Bulletin Paper
Author Tanizaki, Yoshiro| Mitsunobu, Fumihiro| Mifune, Takashi| Hosaki, Yasuhiro| Ashida, Kozo| Tsugeno, Hirofumi| Okamoto, Makoto| Harada, Seishi| Yumoto, Eiichiro| Takata, Shingo| Ochi, Koji| Harada, Hideo|
Published Date 1998-12
Publication Title 岡大三朝分院研究報告
Volume volume69
Content Type Departmental Bulletin Paper
Author Tanizaki, Yoshiro| Mitsunobu, Fumihiro| Mifune, Takashi| Hosaki, Yasuhiro| Ashida, Kozo| Tsugeno, Hirofumi| Okamoto, Makoto| Harada, Seishi| Yumoto, Eiichiro| Takata, Shingo| Ochi, Koji| Harada, Hideo|
Published Date 1998-12
Publication Title 岡大三朝分院研究報告
Volume volume69
Content Type Departmental Bulletin Paper
Author Takata, Shingo| Yumoto, Eiichirou| Okamoto, Makoto| Tsugeno, Hirofumi| Harada, Seishi| Hosaki, Yasuhiro| Mifune, Takashi| Mitsunobu, Fumihiro| Tanizaki, Yoshiro| Harada, Mine|
Published Date 1999-12
Publication Title 岡大三朝分院研究報告
Volume volume70
Content Type Departmental Bulletin Paper
Author Harada, Seishi| Tsugeno, Hirofumi| Mitsunobu, Fumihiro| Mifune, Takashi| Hosaki, Yasuhiro| Ashida, Kozo| Okamoto, Makoto| Takata, Shingo| Yamamoto, Toshio| Tanizaki, Yoshiro|
Published Date 1999-12
Publication Title 岡大三朝分院研究報告
Volume volume70
Content Type Departmental Bulletin Paper
Author Tsugeno, Hirofumi| Nakai, Mutsuro| Okamoto, Makoto| Harada, Seishi| Takata, Shingo| Mifune, Takashi| Mitsunobu, Fumihiro| Ashida, Kozo| Hosaki, Yasuhiro| Akiyama, Tsuneo| Tsuji, Takao| Tanizaki, Yoshiro|
Published Date 1999-12
Publication Title 岡大三朝分院研究報告
Volume volume70
Content Type Departmental Bulletin Paper
Author Okamoto, Makoto| Mitsunobu, Fumihiro| Ashida, Kozo| Mifune, Takashi| Hosaki, Yasuhiro| Tsugeno, Hirofumi| Harada, Seishi| Takada, Shingo| Tanizaki, Yoshiro| Kataoka, Mikio| Niiya, Kenji| Harada, Mine|
Published Date 1999-12
Publication Title 岡大三朝分院研究報告
Volume volume70
Content Type Departmental Bulletin Paper
Author Hosaki, Yasuhiro| Takata, Shingo| Mitsunobu, Fumihiro| Mifune, Takashi| Ashida, Kozo| Tsugeno, Hirofumi| Okamoto, Makoto| Harada, Seishi| Tanizaki, Yoshiro| Ochi, Koji| Tsuji, Takao|
Published Date 1999-12
Publication Title 岡大三朝分院研究報告
Volume volume70
Content Type Departmental Bulletin Paper
Author Mifune, Takashi| Takata, Shingo| Okamoto, Makoto| Tsugeno, Hirofumi| Ashida, Kozo| Harada, Seishi| Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Tanizaki, Yoshiro| Harada, Mine|
Published Date 1999-12
Publication Title 岡大三朝分院研究報告
Volume volume70
Content Type Departmental Bulletin Paper
Author Tanizaki, Yoshiro| Mitsunobu, Fumihiro| Mifune, Takashi| Hosaki, Yasuhiro| Ashida, Kozo| Tsugeno, Hirofumi| Okamoto, Makoto| Harada, Seishi| Takata, Shingo| Ochi, Koji| Koide, Norio|
Published Date 1999-12
Publication Title 岡大三朝分院研究報告
Volume volume70
Content Type Departmental Bulletin Paper