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ID 54772
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Author
Itano, Satsuki Graduate School of Environmental and life Science, Okayama University
Maeda, Megumi Graduate School of Environmental and life Science, Okayama University Kaken ID
Md. Ziaur Rahman Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission
Kimura, Yoshinobu Graduate School of Environmental and life Science, Okayama University Kaken ID
Abstract
 We have identified, and purified to homogeneity, a high molecular weight Ginkgo biloba α-fucosidase (α-fucosidase Gb, 120 kDa estimated by SDS‒PAGE) with activity against α-fucosylated oligosaccharides. When a Lewis a epitope-containing N-glycan was used as a substrate, α-fucosidase Gb showed optimum activity at approximately pH 5.5, suggesting that it functions in acidic environments such as the vacuole. It remains uncertain, however, whether this Ginkgo α-fucosidase belongs to the GH29 family, since its N-terminal sequence could not be determined, probably due to a chemical modification. α-Fucosidase Gb showed substantial activity towards the α1,3-fucosyl linkage in Lacto-N-fucopentaose III and an α1,4-fucosyl linkage in the Lewis a epitope found in plant complex type N-glycans, indicating an involvement in the degradation process of α-fucosylated oligosaccharides or N-glycoproteins.
Abstract Alternative
 銀杏種子から高分子量 (SDS-PAGE で120 kDa) を有し,α-フコース含有オリゴ糖に活性を示すα-フコシダーゼ(α-fucosidase Gb)を均一に精製した.ルイス a エピトープ含有 N-グリカンを基質とした場合,α-fucosidase Gb の至適 pH は 5.5 付近であることから,本酵素は液胞のような酸性環境で機能していることが示唆された.N‒末端アミノ酸配列が化学修飾のため同定できなかったため,本酵素が GH29 ファミリーに属するかどうかは不明である.α-Fucosidase Gb は,Lacto-N-fucopentaose IIIの α1,3-フコース残基やルイス a エピトープ含有の植物複合型N-グリカンのα1,4-フコース残基を加水分解することから,α-フコース含有オリゴ糖やN 型糖タンパク質の分解プロセスに関与することが示唆された.
Keywords
α-fucosidase
plant N-glycan
N-glycan degradation
Ginkgo biloba
Note
原著論文 (Original paper)
Published Date
2017-02-01
Publication Title
Scientific Reports of the Faculty of Agriculture, Okayama University
Volume
volume106
Publisher
岡山大学農学部
Start Page
5
End Page
12
ISSN
2186-7755
Content Type
Departmental Bulletin Paper
language
英語
File Version
publisher
Refereed
False
Eprints Journal Name
srfa