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ID 59967
Author
Nemoto, Michiko Graduate School of Environmental and Life Science, Okayama University Kaken ID researchmap
Iwaki, Sayako Graduate School of Environmental and Life Science, Okayama University
Moriya, Hisao Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Monden, Yuki Graduate School of Environmental and Life Science, Okayama University Kaken ID publons researchmap
Tamura, Takashi Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Inagaki, Kenji Graduate School of Environmental and Life Science, Okayama University Kaken ID researchmap
Mayama, Shigeki Department of Biology, Tokyo Gakugei University
Obuse, Kiori Graduate School of Environmental and Life Science, Okayama University Kaken ID researchmap
Abstract
Silica cell walls of diatoms have attracted attention as a source of nanostructured functional materials and have immense potential for a variety of applications. Previous studies of silica cell wall formation have identified numerous involved proteins, but most of these proteins are species-specific and are not conserved among diatoms. However, because the basic process of diatom cell wall formation is common to all diatom species, ubiquitous proteins and molecules will reveal the mechanisms of cell wall formation. In this study, we assembled de novo transcriptomes of three diatom species, Nitzschia palea, Achnanthes kuwaitensis, and Pseudoleyanella lunata, and compared protein-coding genes of five genome-sequenced diatom species. These analyses revealed a number of diatom-specific genes that encode putative endoplasmic reticulum-targeting proteins. Significant numbers of these proteins showed homology to silicanin-1, which is a conserved diatom protein that reportedly contributes to cell wall formation. These proteins also included a previously unrecognized SET domain protein methyltransferase family that may regulate functions of cell wall formation-related proteins and long-chain polyamines. Proteomic analysis of cell wall-associated proteins in N. palea identified a protein that is also encoded by one of the diatom-specific genes. Expression analysis showed that candidate genes were upregulated in response to silicon, suggesting that these genes play roles in silica cell wall formation. These candidate genes can facilitate further investigations of silica cell wall formation in diatoms.
Keywords
Biomineralization
Diatom
Silica
Transcriptome
Proteome
Note
This is a post-peer-review, pre-copyedit version of an article published in Marine Biotechnology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10126-020-09976-1.
This fulltext is available in June 2021.
Published Date
2020-06-03
Publication Title
Marine Biotechnology
Publisher
Springer
ISSN
1436-2228
NCID
AA11357643
Content Type
Journal Article
language
フランス語
OAI-PMH Set
岡山大学
File Version
author
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1007/s10126-020-09976-1
Funder Name
Ministry of Education, Culture, Sports, Science and Technology
助成番号
18K05818