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ID 57930
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Ogawa, Hirohito Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kaken ID researchmap
Hirayama, Haruko Department of Animal Resources, Advanced Science Research Center, Okayama University Kaken ID researchmap
Tanaka, Satsuki Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Yata, Norio Department of Animal Resources, Advanced Science Research Center, Okayama University
Namba, Hikaru Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID publons researchmap
Yamashita, Nobuko Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kaken ID publons researchmap
Yonemitsu, Kenzo Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University
Maeda, Ken Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University
Mominoki, Katsumi Department of Animal Resources, Advanced Science Research Center, Okayama University Kaken ID publons researchmap
Yamada, Masao Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Kaken ID publons researchmap
Abstract
Hepatitis E virus (HEV) is known to cause zoonotic infections from pigs, wild boars and deer. Domestic pigs have been used as an experimental animal model in medical research and training; however, the risks of HEV infection from pigs during animal experiments are largely unknown. Here, we retrospectively investigated the seroprevalence and detection rates of viral RNA in 73 domestic pigs (average 34.5 kg) introduced into an animal experimental facility in a medical school during 2012-2016. We detected anti-HEV immunoglobulin G antibodies in 24 of 73 plasma samples (32.9%), though none of the samples were positive for viral RNA. Plasma samples of 18 pigs were sequentially monitored and were classified into four patterns: sustained positive (5 pigs), sustained negative (5 pigs), conversion to positive (6 pigs) and conversion to negative (2 pigs). HEV genomes were detected in 2 of 4 liver samples from pigs that were transported from the same farm during 2016-2017. Two viral sequences of the overlapping open reading frame (ORF) 2/3 region (97 bp) were identical and phylogenetically fell into genotype 3. A 459-bp length of the ORF2 region of an amplified fragment from a pig transported in 2017 was clustered with the wbJYG1 isolate (subgenotype 3b) with 91.5% (420/459 bp) nucleotide identity. Based on our results, we suggest that domestic pigs introduced into animal facilities carry a potential risk of HEV infection to researchers, trainees and facility staff. Continuous surveillance and precautions are important to prevent HEV infection in animal facilities.
Keywords
animal experimental facility
domestic pig
hepatitis E virus
zoonosis
Published Date
2019-08-24
Publication Title
Journal of Veterinary Medical Science
Volume
volume81
Issue
issue8
Publisher
The Japanese Society of Veterinary Science
Start Page
1191
End Page
1196
ISSN
0916-7250
NCID
AA10796138
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
©2019 The Japanese Society of Veterinary Science
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publisher
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1292/jvms.19-0086
License
https://creativecommons.org/licenses/by-nc-nd/4.0/
Citation
Hirohito OGAWA, Haruko HIRAYAMA, Satsuki TANAKA, Norio YATA, Hikaru NAMBA, Nobuko YAMASHITA, Kenzo YONEMITSU, Ken MAEDA, Katsumi MOMINOKI, Masao YAMADA, Risk assessment for hepatitis E virus infection from domestic pigs introduced into an experimental animal facility in a medical school, Journal of Veterinary Medical Science, 2019, Volume 81, Issue 8, Pages 1191-1196, Released August 24, 2019, [Advance publication] Released July 08, 2019, Online ISSN 1347-7439, Print ISSN 0916-7250, https://doi.org/10.1292/jvms.19-0086
Funder Name
Japan Society for the Promotion of Science
助成番号
25930022