このエントリーをはてなブックマークに追加
ID 60778
FullText URL
fulltext.pdf 2.16 MB
Author
Mitalo, Oscar W. Graduate School of Environmental and Life Science, Okayama University
Otsuki, Takumi Graduate School of Environmental and Life Science, Okayama University
Okada, Rui Graduate School of Environmental and Life Science, Okayama University
Obitsu, Saeka Graduate School of Environmental and Life Science, Okayama University
Masuda, Kanae Graduate School of Environmental and Life Science, Okayama University
Hojo, Yuko Institute of Plant Science and Resources, Okayama University
Matsuura, Takakazu Institute of Plant Science and Resources, Okayama University
Mori, Izumi C. Institute of Plant Science and Resources, Okayama University ORCID Kaken ID publons researchmap
Abe, Daigo National Agriculture and Food Research Organization, Shikoku Research Station
Asiche, William O. Department of Research and Development, Del Monte Kenya Ltd
Akagi, Takashi Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID researchmap
Kubo, Yasutaka Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Ushijima, Koichiro Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Abstract
Peel degreening is an important aspect of fruit ripening in many citrus fruit, and previous studies have shown that it can be advanced by ethylene treatment or by low-temperature storage. However, the important regulators and pathways involved in natural peel degreening remain largely unknown. To determine how natural peel degreening is regulated in lemon fruit (Citrus limon), we studied transcriptome and physiochemical changes in the flavedo in response to ethylene treatment and low temperatures. Treatment with ethylene induced rapid peel degreening, which was strongly inhibited by the ethylene antagonist, 1-methylcyclopropene (1-MCP). Compared with 25 degrees C, moderately low storage temperatures of 5-20 degrees C also triggered peel degreening. Surprisingly, repeated 1-MCP treatments failed to inhibit the peel degreening induced by low temperature. Transcriptome analysis revealed that low temperature and ethylene independently regulated genes associated with chlorophyll degradation, carotenoid metabolism, photosystem proteins, phytohormone biosynthesis and signalling, and transcription factors. Peel degreening of fruit on trees occurred in association with drops in ambient temperature, and it coincided with the differential expression of low temperature-regulated genes. In contrast, genes that were uniquely regulated by ethylene showed no significant expression changes during on-tree peel degreening. Based on these findings, we hypothesize that low temperature plays a prominent role in regulating natural peel degreening independently of ethylene in citrus fruit.
Keywords
1-methylcyclopropene
carotenoids
chlorophyll
Citrus limon
ethylene
low temperature
peel degreening
phytohormones
transcriptome
Published Date
2020-05-06
Publication Title
Journal of Experimental Botany
Volume
volume71
Issue
issue16
Publisher
Oxford University Press
Start Page
4778
End Page
4796
ISSN
0022-0957
NCID
AA00697526
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© The Author(s) 2020.
File Version
publisher
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1093/jxb/eraa206
License
http://creativecommons.org/licenses/by/4.0/
Funder Name
Japan Society for the Promotion of Science
助成番号
24380023
16H04873
20H02977