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ID 52795
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Author
Hirai, Misato
Fukushima, Kunihiro
Sugaya, Akiko
Nishizaki, Kazunori Kakenhi
Abstract
MicroRNAs (miRNAs) play functional roles in sound transduction in cochlea. This study focuses on the validity of cochlear culture as an in vitro experimental tool, in view of miRNA expression. E15 cochleae were dissected and maintained in vitro for 48 h before extraction of miRNAs. MiRNA expression was comprehensively screened in explanted cochleae using a miRNA array that covers 380 miRNAs. A strong correlation was observed between expression levels of miRNAs in in vitro and in in vivo cochleae. Levels of 43 miRNAs were altered in vitro and these changes were reproducible over three trials. These findings indicate that in vitro miRNA profiling is a viable method for analysis of gene expression and action of chemical compounds on cochleae.
Keywords
embryonic cochlea
microRNA array
organ culture of cochlea
Published Date
2011-09-14
Publication Title
NeuroReport
Volume
volume22
Issue
issue13
Start Page
652
End Page
654
ISSN
0959-4965
Content Type
Journal Article
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/52518
language
英語
Copyright Holders
(C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
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author
Refereed
True
DOI
Web of Science KeyUT