| Author | 西田 圭一郎| |
|---|---|
| Published Date | 1995-09-30 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| JaLCDOI | 10.18926/AMO/30384 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Nishida, Keiichiro| Inoue, Hajime| Toda, Kazukiyo| Murakami, Takuro| |
| Abstract | <p>Localization of the glycosaminoglycans (GAG) was examined in the synovial membranes of patients with osteoarthritis under light microscopy using a fine cationic colloidal iron staining method combined with enzymatic digestion. Our staining method was very useful for demonstrating the difference in the localization of GAG in regions of the inflammatory site in the osteoarthritic synovial membrane. Hyaluronic acid was mainly located in connective tissues in the surface intercellular and perivascular spaces, chondroitin sulfate A/C in the highly fibrous part of and connective tissue around blood vessels, dermatan sulfate (chondroitin sulfate B) in the subsurface interstitium and vascular endothelial cells and heparan sulfate in part of vascular endothelial cells. No keratan sulfate was detected. GAG is reported to have an important role in cell movement, adherence and aggregation in the inflammatory sites. These findings should be useful for understanding the role of GAG in physiological and pathologic processes of secondary synovitis.</p> |
| Keywords | glycosaminoglycan synovial tissue osteoarthritis fine cationic colloidal iron |
| Amo Type | Article |
| Published Date | 1995-12 |
| Publication Title | Acta Medica Okayama |
| Volume | volume49 |
| Issue | issue6 |
| Publisher | Okayama University Medical School |
| Start Page | 287 |
| End Page | 294 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 8770237 |
| Web of Science KeyUT | A1995TM84600003 |
| Author | Murakami, Takuro| Piao, Da Xun| Ohtsuka, Aiji| Nishida, Keiichiro| |
|---|---|
| Published Date | 1996-10-31 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume108 |
| Issue | issue9-10 |
| Content Type | Journal Article |
| JaLCDOI | 10.18926/AMO/30778 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Kinugasa, Kiyoto| Hashizume, Hiroyuki| Nishida, Keiichiro| Shigeyama, Yukio| Inoue, Hajime| |
| Abstract | <p>The results of the histological examinations of specimens of the tenosynovium of the flexor tendon, the epineurium and the transverse carpal ligament from two groups of Japanese patients with carpal tunnel syndrome (idiopathic and hemodialysis) were compared. Amyloid deposits, positively identified as β<sub>2</sub>-microglobulin, appeared in all patients in the long-term hemodialysis group, but in no patients in the idiopathic group. Although the pathogenesis differed between the two groups, both resulted in nerve compression in the carpal tunnel. Therefore, surgical release is considered beneficial for both groups.</p> |
| Keywords | carpal tunnel syndrome histopathology clinical results idiopathic hemodialysis |
| Amo Type | Article |
| Published Date | 1997-04 |
| Publication Title | Acta Medica Okayama |
| Volume | volume51 |
| Issue | issue2 |
| Publisher | Okayama University Medical School |
| Start Page | 63 |
| End Page | 70 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 9142342 |
| Web of Science KeyUT | A1997WX19600002 |
| JaLCDOI | 10.18926/AMO/30794 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Nagoshi, Mitsuru| Hashizume, Hiroyuki| Nishida, Keiichiro| Takagoshi, Hidekazu| Pu, Jean| Inoue, Hajime| |
| Abstract | <p>Sixty-seven trigger fingers of 58 idiopathic and hemodialysis patients were treated by percutaneous A1-pulley release technique. Severity of triggering was classified into five grades for treatment selection and prediction of possible results. Results were excellent in 41 fingers, good in 9, fair in 7, and poor in 10, requiring additional treatment. The results of the lower grades were better, and those of the higher grades were poor. Excellent or good results appeared to depend on the proper selection of the patients according to the grading system and confirmation of triggering disappearance just after the release. There were neither infections nor neuro-vascular deficits after treatment. Compared to conventional open release, this treatment was found to be more useful from the standpoints of ease and safety of the technique, and the patients' quick return to normal life.</p> |
| Keywords | trigger finger percutaneous release idiopathic hemodialysis |
| Amo Type | Article |
| Published Date | 1997-06 |
| Publication Title | Acta Medica Okayama |
| Volume | volume51 |
| Issue | issue3 |
| Publisher | Okayama University Medical School |
| Start Page | 155 |
| End Page | 158 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 9227795 |
| Web of Science KeyUT | A1997XJ12700007 |
| JaLCDOI | 10.18926/AMO/30790 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Nanba, Yoshifumi| Nishida, Keiichiro| Yoshikawa, Takeshi| Sato, Toru| Inoue, Hajime| Kuboki, Yoshinori| |
| Abstract | <p>The expression of osteonectin (ON) in osteoarthritic articular cartilage was investigated by enzyme immunohistochemistry and colloidal gold immunoelectron microscopy. A total of 96 specimens from 9 knees of 8 patients with osteoarthritis (OA) were examined. In OA cartilage, ON-positive cells varied in distribution and were not seen in all the specimens obtained from the same patient. However, in over half of the specimens (56 of 96), especially in the specimens of Mankin's grades from 4 to 9, which corresponds to relatively early stages of OA, ON was expressed in the cartilage above the calcified layer. On the other hand, ON was detected only in the calcified layer below the tidemark in normal articular cartilage. In addition, colloidal gold immunoelectron microscopy revealed ON in chondrocytes and matrix vesicles (MVs). These findings suggest that ON acts through MVs in the early stages of OA as a significant pathogenetic factor involved in intracartilage calcification, which is known to have a close relationship to the progression of OA.</p> |
| Keywords | chondrocalcinosis osteoarthritis osteonection Mankin's histologic-histochemical grading calcification |
| Amo Type | Article |
| Published Date | 1997-10 |
| Publication Title | Acta Medica Okayama |
| Volume | volume51 |
| Issue | issue5 |
| Publisher | Okayama University Medical School |
| Start Page | 239 |
| End Page | 243 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 9359920 |
| Web of Science KeyUT | A1997YD65300001 |
| JaLCDOI | 10.18926/AMO/31338 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Takagoshi, Hidekazu| Hashizume, Hiroyuki| Nishida, Keiichiro| Masaoka, shunji| Asahara, Hiroshi| Inoue, Hajime| |
| Abstract | <p>The fibrous components of the metacarpophalangeal (MP) joint including the palmar plate, the collateral ligament and the dorsal plate were studied with particular attention paid to the fibrous structure of the fibrous tendon sheath and the deep transverse metacarpal ligament. The tough fibrillar structure around the MP joint, especially the force nucleus, consisted of three types of mixed fibers: the fibrous tendon sheath of the A1 pulley, the deep transverse metacarpal ligament, and the palmar plate. The tendon sheath was located on the ulnar side in the index and middle fingers, on the central position in the ring finger, and on the radial side in the little finger. These fibrous connections among the fingers formed a transverse arch in the hand. The palmar plate of the MP joint was relatively rigid and appears to function as a cushion when flexed. A fold-like protrusion of the synovial layer of the palmar plate of the MP joint had a meniscoid function, which was larger than that of the proximal interphalangeal joint. The capsule of the MP joint was thicker at the dorsal area, forming a dorsal plate, which is a sliding floor of the extensor mechanism and has a meniscoid function for joint congruity. The main lateral stabilizer consisted of collateral ligaments and accessory collateral ligaments anchored to the palmar plate. These structures act together as a "phalangeal cuff", connecting the proximal phalanx to the metacarpal head and sta</p> |
| Keywords | metacarpophalangeal joint collagen framework |
| Amo Type | Article |
| Published Date | 1998-02 |
| Publication Title | Acta Medica Okayama |
| Volume | volume52 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 19 |
| End Page | 26 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 9548990 |
| Web of Science KeyUT | 000072264100003 |
| JaLCDOI | 10.18926/AMO/31339 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Pu, Jian| Nishida, Keiichiro| Inoue, Hajime| Asahara, Hiroshi| Ohtsuka, Aiji| Murakami, Takuro| |
| Abstract | <p>Mast cells in osteoarthritic and rheumatoid arthritic synovial tissues of the human knee.</p> |
| Keywords | mast cell count osteoaethritis rheumatoid arthritis hydroarthrosis synovial membrance |
| Amo Type | Article |
| Published Date | 1998-02 |
| Publication Title | Acta Medica Okayama |
| Volume | volume52 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 35 |
| End Page | 39 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 9548992 |
| Web of Science KeyUT | 000072264100005 |
| JaLCDOI | 10.18926/AMO/31296 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Takeuchi, Kazuhiro| Inoue, Hajime| Yokoyama, Yoshiki| Senda, Masuo| Ota, Yusuke| Abe, Nobuhiro| Nishida, Keiichiro| |
| Abstract | <p>We studied the magnetic resonance imaging (MRI) of 120 knees in 86 rheumatoid arthritis (RA) patients and of 14 unaffected knees in 12 control cases. We also developed a scoring system as a quantitative analysis method. We divided the MRI into 10 items, and classified the severity of the symptoms into 4 grades (score 0 to 3). The average total score increased according to the radiographic grade. Soft tissue lesions were clearly detected, even in the early stages of RA. Items such as synovial proliferation showed a high score even in the early stages, suggesting that it was the initial symptom of RA. The score also showed a correlation with the inflammatory signs. These results suggest that this scoring system is very sensitive and yields a good reflection of RA activity. We demonstrated that this system is simple and convenient for routine diagnostic use. We further demonstrated that it is useful for following the advancement of RA and for evaluating the response to treatment.</p> |
| Keywords | rheumatoid arthritis magnetic resonance imaging scoring system synovial membrane |
| Amo Type | Article |
| Published Date | 1998-08 |
| Publication Title | Acta Medica Okayama |
| Volume | volume52 |
| Issue | issue4 |
| Publisher | Okayama University Medical School |
| Start Page | 211 |
| End Page | 224 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 9781272 |
| Web of Science KeyUT | 000075623600006 |
| JaLCDOI | 10.18926/AMO/31628 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Masaoka, Shunji| Hashizume, Hiroyuki| Senda, Masuo| Nishida, Keiichiro| Nagoshi, Mitsuru| Inoue, Hajime| |
| Abstract | <p>Seventy-nine shoulders suspected of rotator cuff tears were examined by ultrasonography (US) and forty-three received surgery. Long and short axis scans were performed and findings of each were separately classified according to a five-grade system, and the results were correlated with the actual extent of tear observed during surgery. Internal echogenicity and subacromial impingement were analyzed before and after surgery. A accuracy of US in detecting rotator cuff tears was analyzed. In addition, the correlation between cuff shape observed by US before surgery and actual shape observed during surgery was assessed. It was noted that cuff thinning and abnormalities in shape did not recover to normal after surgery. However, in the cases of discontinuities observed by US before surgery, US findings indicated that the torn cuff was anchored to the greater tuberosity and functional during active motion. Although post-operative US findings were not normal, clinical results were good in most cases. Sensitivity of US for detecting rotator cuff tear was 100% and specificity 94%. US is non-invasive, cost effective and allows the physician to examine the joint while it is in motion. Therefore, at this time, we use US as a screening method for detecting rotator cuff tears. Furthermore, US allows us to check for re-tears while the joint is in motion, which is essential for accurate diagnosis.</p> |
| Keywords | rotator cuff tear ultrasonography diagnostic image |
| Amo Type | Article |
| Published Date | 1999-04 |
| Publication Title | Acta Medica Okayama |
| Volume | volume53 |
| Issue | issue2 |
| Publisher | Okayama University Medical School |
| Start Page | 81 |
| End Page | 89 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| Web of Science KeyUT | 000080058700004 |
| JaLCDOI | 10.18926/AMO/32287 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Shibahara, Motomi| Nishida, Keiichiro| Asahara, Hiroshi| Yoshikawa, Takeshi| Mitani, Shigeru| Kondo, Yoichi| Inoue, Hajime| |
| Abstract | <p>We investigated the presence of osteocyte apoptosis in the necrotic trabeculae of the femoral head of spontaneously hypertensive rat (SHR) using the in situ nick end labeling (TUNEL) method and transmission electron microscopy. The occurrence of osteonecrosis and ossification disturbance was significantly higher in SHR compared with Wistar Kyoto (WKY) rats, and Wistar (WT) rats used as control animals (P < 0.01). A high population of TUNEL positive osteocytes was detected mainly in 10- and 15-week-old SHRs. Sectioned examination of the femoral head of SHRs and WKY rats by electron microscopy revealed apoptotic cell appearances such as aggregation of chromatin particles and lipid formation. In contrast, a positive reaction was significantly lower in osteocytes in the femoral heads of WT rats (P < 0.01). Our results indicate that apoptosis forms an important component of the global pathologic process affecting the femoral head of SHR, which leads to osteonecrosis in this region.</p> |
| Keywords | apoptosis spontaneously hypertensive rat osteonecrosis of the femoral head |
| Amo Type | Article |
| Published Date | 2000-04 |
| Publication Title | Acta Medica Okayama |
| Volume | volume54 |
| Issue | issue2 |
| Publisher | Okayama University Medical School |
| Start Page | 67 |
| End Page | 74 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 10806527 |
| Web of Science KeyUT | 000086735900003 |
| JaLCDOI | 10.18926/AMO/32000 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Matsuo, Masatsugu| Nishida, Keiichiro| Yoshida, Aki| Murakami, Takuro| Inoue, Hajime| |
| Abstract | <p>To clarify the involvement of the caspase family in the pathway of NO-induced chondrocyte apoptosis, osteoarthritis (OA) cartilage obtained from 8 patients undergoing total hip arthroplasty were used for histopathological study. Cartilage samples taken from non-fibrillated areas of femoral head resected during surgery for femoral neck fracture were used for comparison. DNA fragmentation of chondrocytes was detected by the nick end-labeling (TUNEL) method. Apoptosis was further confirmed by transmission electron microscopy. The distributions of nitrotyrosine (NT), caspase-3, and -9 were examined immunohistochemically. The populations of apoptotic as well as NT-, caspase-3-, and -9-positive cells were quantified by counting the number of cells in the superficial, middle, and deep layers, respectively. The TUNEL-positive cells were observed primarily in superficial proliferating chondrocytes, clustering chondrocytes, and deep-layer chondrocytes of OA cartilage. Few positive cells were seen in the proliferating chondrocytes in the middle layer. Positive reactions for caspase-3 and -9 were observed in chondrocytes in similar areas. Histological OA grade showed significant correlations with the mean populations of apoptotic chondrocytes (% apoptosis) over the 3 areas. The populations of NT-positive cells (% NT) over the same areas also showed significant correlation with OA grade. Positivity for caspase-3 closely correlated with the OA grade, % apoptosis and %NT. It was concluded that caspase-3 and -9 could play a role in NO-induced chondrocyte apoptosis in OA cartilage.</p> |
| Keywords | apoptosis caspase nitric oxide osteoarthritis chondrocyte |
| Amo Type | Article |
| Published Date | 2001-12 |
| Publication Title | Acta Medica Okayama |
| Volume | volume55 |
| Issue | issue6 |
| Publisher | Okayama University Medical School |
| Start Page | 333 |
| End Page | 340 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 11779095 |
| Web of Science KeyUT | 000172838400003 |
| JaLCDOI | 10.18926/AMO/31711 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Kosaka, Motohiro| Horiuchi, Kanji| Nishida, Keiichiro| Taguchi, Takehito| Murakami, Takuro| Ohtsuka, Aiji| |
| Abstract | <p>The celiac and mesenteric arterial system including the left gastric, splenic, common hepatic, and superior mesenteric arteries shows various types of origins, courses, ramifications and anastomoses. In order to explain the various expressions of this system, we have proposed a typological model, in which celiacomesenteric arteries develop as paired or bilaterally symmetrical primordial vessels originated from the anterior aspect of the aorta, and these vessels anastomose each other with longitudinal and horizontal pathways. Here, we report 3 unusual cases characterized by arterial rings, formed by the left gastric, left accessory hepatic, proper hepatic, anterior pancreaticoduodenal, and dorsal pancreatic arteries. The dorsal pancreatic and anterior pancreaticoduodenal arteries are located to the right and left of the embryonic pancreas developing in the dorsal mesentery, respectively. Such hepatopancreatic arterial rings simultaneously containing right and left elements can only be explained using our typological model, in which the concept of paired arteries or bilateral symmetry is introduced.</p> |
| Keywords | arterial variation celiac trunk superior mesenteric artery typology bilateral symmetry |
| Amo Type | Article |
| Published Date | 2002-10 |
| Publication Title | Acta Medica Okayama |
| Volume | volume56 |
| Issue | issue5 |
| Publisher | Okayama University Medical School |
| Start Page | 245 |
| End Page | 253 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 12530508 |
| Web of Science KeyUT | 000178668100005 |
| Author | Demircan, Kadir| Hirohata, Satoshi| Nishida, Keiichiro| Hatipoglu, Omer F.| Oohashi, Toshitaka| Yonezawa, Tomoko| Apte, Suneel S.| Ninomiya, Yoshifumi| |
|---|---|
| Published Date | 2005-5 |
| Publication Title | Arthritis & Rheumatism |
| Volume | volume52 |
| Issue | issue5 |
| Content Type | Journal Article |
| Author | 西田 圭一郎| |
|---|---|
| Published Date | 2006-01-04 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume117 |
| Issue | issue3 |
| Content Type | Journal Article |
| JaLCDOI | 10.18926/AMO/30753 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Nakatani, Satoru| Naito, Ichiro| Momota, Ryusuke| Hinenoya, Noriko| Horiuchi, Kanji| Nishida, Keiichiro| Ohtsuka, Aiji| |
| Abstract | <p>We attempted to prepare colloidal iron within tissues by means of microwave irradiation. Mouse tissue blocks were fixed with a mixture of paraformaldehyde and ferric chloride in a cacodylate buffer, immersed in a cacodylate buffered ferric chloride solution, and irradiated in a microwave processor. Colloidal iron was prepared within tissues or cells, and was observed in the form of electron dense fine granules (1-2 nm in diameter) by transmission electron microscopy. Collagen fibrils in the connective tissue showed colloidal iron deposition at regular periodical intervals. Cells in the splenic tissue showed that fine colloidal granules were deposited on the ribosomes but not on the nuclear chromatin. This finding suggests that ferric ions could not diffuse into the nucleus, which was surrounded by the nuclear envelope. The podocyte processes of the renal glomerulus were stained diffusedly. Though this microwave in situ colloidal iron preparation method has some limitations, it is convenient for use in biomedical specimen preparation in transmission electron microscopy.</p> |
| Keywords | colloidal iron microwave histochemistry transmission electron microscopy |
| Amo Type | Article |
| Published Date | 2006-02 |
| Publication Title | Acta Medica Okayama |
| Volume | volume60 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 59 |
| End Page | 64 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 16508690 |
| Web of Science KeyUT | 000235538900007 |
| JaLCDOI | 10.18926/AMO/30749 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Komiyama, Takamitsu| Nishida, Keiichiro| Yorimitsu, Masanori| Doi, Hideyuki| Miyazawa, Shinichi| Kitamura, Ai| Yoshida, Aki| Nasu, Yoshihisa| Abe, Nobuhiro| Ozaki, Toshifumi| |
| Abstract | Ossification disturbance in femoral head reportedly is seen in the Spontaneously Hypertensive rats (SHR) between ages of 10 and 20 weeks. We investigated serum and tissue levels of insulin-like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) in SHR relevant to the ossification disturbance and osteonecrosis of the femoral head. Serum levels of IGF-1 and VEGF were significantly lower in SHR than in Wistar Kyoto rats (WKY) at weeks 5, 10, 15 and 20 (p<0.005). The incidence of histological ossification disturbance of the femoral head was higher in SHR (59%) than in WKY (40%) at week 20. Lower serum and local levels of VEGF in SHR appeared to be related to the incomplete ossification of the femoral heads. Immunohistochemical study showed significantly lower numbers of IGF-1 and VEGF positive chondrocytes in the femoral epiphyseal cartilage of SHR than in those of WKY at weeks 10, 15 and 20. Our results suggest that local and/or systemic levels of IGF-1 and VEGF between ages of 5 and 20 weeks might play roles in the pathogenesis of ossifi cation disturbance of the femoral head in SHR. |
| Keywords | spontaneous hypertensive rats insulin like growth factor-1 vascular endothelial growth factor ossification disturbance osteonecrosis |
| Amo Type | Article |
| Published Date | 2006-06 |
| Publication Title | Acta Medica Okayama |
| Volume | volume60 |
| Issue | issue3 |
| Publisher | Okayama University Medical School |
| Start Page | 141 |
| End Page | 148 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 16838042 |
| Web of Science KeyUT | 000238503600001 |
| JaLCDOI | 10.18926/AMO/30956 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Doi, Hideyuki| Nishida, Keiichiro| Yorimitsu, Masanori| Komiyama, Takamitsu| Kadota, Yasutaka| Tetsunaga, Tomonori| Yoshida, Aki| Kubota, Satoshi| Takigawa, Masaharu| Ozaki, Toshifumi| |
| Abstract | <p>Mechanical stress plays a key role in the pathogenesis of cartilage destruction seen in osteoarthritis (OA). We investigated the effect of cyclic tensile stress (CTS) on the anabolic and catabolic gene expression of rat cultured normal chondrocytes using the Flexercell strain unit. The effects of interleukin (IL)-4, a chondroprotective cytokine, on the changes in gene expression induced by CTS were also investigated. CTS (7% elongation at 0.5 Hz) for 24 h did not affect the expression of aggrecan and type II collagen, whereas CTS significantly upregulated matrix metalloproteinase (MMP)-13 and cathepsin B mRNA expression by chondrocytes. IL-1beta expression was also signifi cantly upregulated by CTS up to 12 h. The upregulation of MMP-13 was observed at 3 h, which was earlier than that of IL-1beta. Furthermore, pre-treatment with IL-4 (10 ng/ml) suppressed both MMP-13 and cathepsin B induction by mechanical stress, as well as CTS-induced IL-1beta expression. Our results suggest that IL-4 might have a therapeutic value in the treatment of OA by downregulation of mechanical stress-induced MMP-13 and cathepsin B expression by chondrocytes.</p> |
| Keywords | IL-4 MMP cathepsin B mechanical stress aggrecanase |
| Amo Type | Original Article |
| Published Date | 2008-04 |
| Publication Title | Acta Medica Okayama |
| Volume | volume62 |
| Issue | issue2 |
| Publisher | Okayama University Medical School |
| Start Page | 119 |
| End Page | 126 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 18464888 |
| Web of Science KeyUT | 000255297600008 |
| JaLCDOI | 10.18926/AMO/30974 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Ikuma, Hisanori| Abe, Nobuhiro| Uchida, Youichiro| Furumatsu, Takayuki| Fujiwara, Kazuo| Nishida, Keiichiro| Ozaki, Toshifumi| |
| Abstract | <p>Instability of the knee after the medial collateral ligament (MCL) injury is usually assessed with the manual valgus stress test, even though, in recent years, it has become possible to apply magnetic resonance imaging (MRI) to the assessment of the damage of the ligament. The valgus instability of 24 patients (12 isolated injuries and 12 multiple ligament injuries) who suffered MCL injury between 1993 and 1998 was evaluated with the Hughston and Eilers classification, which involves radiographic assessment under manual valgus stress to the injured knees. We developed a novel system for classifying the degree of injury to the MCL by calculating the percentage of injured area based on MRI and investigated the relationship between this novel MRI classification and the magnitude of valgus instability by the Hughston and Eilers classification. There was a significant correlation between the 2 classifications (p=0.0006). On the other hand, the results using other MRI based classification systems, such as the Mink and Deutsch classificaiton and the Petermann classification, were not correlated with the findings by the Hughston and Eilers classification in these cases (p0.05). Since MRI is capable of assessing the injured ligament in clinical practice, this novel classification system would be useful for evaluating the stability of the knee and choosing an appropriate treatment following MCL injury.</p> |
| Keywords | medial collateral ligament magnetic resonance imaging knee instability novel method |
| Amo Type | Original Article |
| Published Date | 2008-06 |
| Publication Title | Acta Medica Okayama |
| Volume | volume62 |
| Issue | issue3 |
| Publisher | Okayama University Medical School |
| Start Page | 185 |
| End Page | 191 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 18596835 |
| Web of Science KeyUT | 000257130300006 |
| Author | Tetsunaga, Tomonori| Furumatsu, Takayuki| Abe, Nobuhiro| Nishida, Keiichiro| Naruse, Keiji| Ozaki, Toshifumi| |
|---|---|
| Published Date | 2009-09-18 |
| Publication Title | Journal of Biomechanics |
| Volume | volume42 |
| Issue | issue13 |
| Content Type | Journal Article |
