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ID 48972
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Author
Hayashi, Nobuyoshi
Kuroda, Yuka
Takiue, Chisato
Hirata, Rei
Takenami, Mami
Aoi, Yoko
Yoshioka, Nanako
Habara, Toshihiro
Mukaida, Tetsunori
Abstract
Mammalian embryos experience not only hormonal but also mechanical stimuli, such as shear stress, compression and friction force in the Fallopian tube before nidation. In order to apply mechanical stimuli to embryos in a conventional IVF culture system, the tilting embryo culture system (TECS) was developed. The observed embryo images from the TECS suggest that the velocities and shear stresses of TECS embryos are similar to those experienced in the oviduct. Use of TECS enhanced the development rate to the blastocyst stage and significantly increased the cell number of mouse blastocysts (P < 0.05). Although not statistically significant, human thawed embryos showed slight improvement in development to the blastocyst stage following culture in TECS compared with static controls. Rates of blastocyst formation following culture in TECS were significantly improved in low-quality embryos and those embryos cultured under suboptimal conditions (P < 0.05). The TECS is proposed as a promising approach to improve embryo development and blastocyst formation by exposing embryos to mechanical stimuli similar to those in the Fallopian tube.
Keywords
blastocyst
embryo development
mechanical stimuli
shear stress
tilting embryo culture system
Published Date
2010-03
Publication Title
Reproductive BioMedicine Online
Volume
volume20
Issue
issue3
Publisher
Elsevier
Start Page
358
End Page
364
ISSN
1472-6483
Content Type
Journal Article
Project
Research Core for Interdisciplinary Sciences
Official Url
http://www.sciencedirect.com/science/article/pii/S1472648309002727
language
英語
Copyright Holders
Copyright © 2009 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
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True
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