Author Furumatsu, Takayuki| Hachioji, Motomi| Saiga, Kenta| Takata, Naoki| Yokoyama, Yusuke| Ozaki, Toshifumi|
Published Date 2010-01-01
Publication Title Biochemical and Biophysical Research Communications
Volume volume391
Issue issue1
Content Type Journal Article
Author Saiga, Kenta| Furumatsu, Takayuki| Yoshida, Aki| Masuda, Shin| Takihira, Shota| Abe, Nobuhiro| Ozaki, Toshifumi|
Published Date 2010-11-12
Publication Title Biochemical and Biophysical Research Communications
Volume volume402
Issue issue2
Content Type Journal Article
Author Miyake, Yoshiaki| Furumatsu, Takayuki| Kubota, Satoshi| Kawata, Kazumi| Ozaki, Toshifumi| Takigawa, Masaharu|
Published Date 2011-06-03
Publication Title Biochemical and Biophysical Research Communications
Volume volume409
Issue issue2
Content Type Journal Article
Author Fujiwara, Kazuo| Endo, Hirosuke| Miyake, Yoshiaki| Ozaki, Toshifumi|
Published Date 2010-08-02
Publication Title 岡山医学会雑誌
Volume volume122
Issue issue2
Content Type Journal Article
Author Furumatsu, Takayuki| Ozaki, Toshifumi| Asahara, Hiroshi|
Published Date 2010-08-02
Publication Title 岡山医学会雑誌
Volume volume122
Issue issue2
Content Type Journal Article
JaLCDOI 10.18926/AMO/40007
FullText URL fulltext.pdf
Author Furumatsu, Takayuki| Ozaki, Toshifumi|
Abstract Epigenetics is an essential mechanism to control gene expression and fundamental cellular processes. DNA methylation in CpG-rich promoters correlates with gene silencing. Histone modification including histone acetylation and deacetylation determines the stability of the chromatin structure. Condensed chromatin (heterochromatin), which has a higher-order histone-DNA structure, prevents the access of transcriptional activators to their target genes. The fundamental unit of eukaryotic chromatin consists of 146 bp of DNA wrapped around a histone octamer. Posttranslational modifications of the histone tail and the chromatin remodeling complex disrupt histone-DNA contacts and induce nucleosome mobilization. Histone acetylation of specific lysine residues in the histone tail plays a crucial role in epigenetic regulation. Histone acetylation is a dynamic process regulated by the antagonistic actions of 2 families of enzymes - the histone acetyltransferases (HATs) and the histone deacetylases (HDACs). The balance between histone acetylation and deacetylation serves as a key epigenetic mechanism for transcription factor-dependent gene expression and the developmental process. We review emerging evidence that DNA methylation, histone acetylation modified by HAT and/or HDAC, and transcription factor-associated molecules contribute to a mechanism that can alter chromatin structure, gene expression, and cellular differentiation during chondrogenesis.
Keywords epigenetics DNA methylation histone acetylation and HAT histone deacetylation and HDAC chondrogenesis
Amo Type Review
Published Date 2010-06
Publication Title Acta Medica Okayama
Volume volume64
Issue issue3
Publisher Okayama University Medical School
Start Page 155
End Page 161
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 20596126
Web of Science KeyUT 000279094300001
JaLCDOI 10.18926/AMO/30974
FullText URL fulltext.pdf
Author Ikuma, Hisanori| Abe, Nobuhiro| Uchida, Youichiro| Furumatsu, Takayuki| Fujiwara, Kazuo| Nishida, Keiichiro| Ozaki, Toshifumi|
Abstract <p>Instability of the knee after the medial collateral ligament (MCL) injury is usually assessed with the manual valgus stress test, even though, in recent years, it has become possible to apply magnetic resonance imaging (MRI) to the assessment of the damage of the ligament. The valgus instability of 24 patients (12 isolated injuries and 12 multiple ligament injuries) who suffered MCL injury between 1993 and 1998 was evaluated with the Hughston and Eilers classification, which involves radiographic assessment under manual valgus stress to the injured knees. We developed a novel system for classifying the degree of injury to the MCL by calculating the percentage of injured area based on MRI and investigated the relationship between this novel MRI classification and the magnitude of valgus instability by the Hughston and Eilers classification. There was a significant correlation between the 2 classifications (p=0.0006). On the other hand, the results using other MRI based classification systems, such as the Mink and Deutsch classificaiton and the Petermann classification, were not correlated with the findings by the Hughston and Eilers classification in these cases (p0.05). Since MRI is capable of assessing the injured ligament in clinical practice, this novel classification system would be useful for evaluating the stability of the knee and choosing an appropriate treatment following MCL injury.</p>
Keywords medial collateral ligament magnetic resonance imaging knee instability novel method
Amo Type Original Article
Published Date 2008-06
Publication Title Acta Medica Okayama
Volume volume62
Issue issue3
Publisher Okayama University Medical School
Start Page 185
End Page 191
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 18596835
Web of Science KeyUT 000257130300006
JaLCDOI 10.18926/AMO/58274
FullText URL 74_2_151.pdf
Author Yamamoto, Norio| Dan’ura, Tomoyuki| Noda, Tomoyuki| Ozaki, Toshifumi|
Abstract This is the second report of an atypical peri-implant femoral fracture occurring at the distal screw after intramedullary nail fixation of a pertrochanteric fracture. A 94-year-old Japanese female with a 5-year history of alendronate intake presented with prodromal pain in her right thigh after intramedullary nail fixation. Plain radiographs showed an incomplete noncomminuted transverse fracture at the distal screw, suggesting an atypical peri-implant femoral fracture. The pathogenesis of an atypical peri-implant femoral fracture could be a combined systemic bone metabolism disorder and repetitive overloading at the screw, similar to the pathogenesis of an atypical periprosthetic femoral fracture around stem implantation.
Keywords atypical femoral fracture insufficiency fracture bisphosphonate atypical peri-implant femoral fracture, atypical periprosthetic femoral fracture
Amo Type Case Report
Published Date 2020-04
Publication Title Acta Medica Okayama
Volume volume74
Issue issue2
Publisher Okayama University Medical School
Start Page 151
End Page 157
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2020 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 32341590
Web of Science KeyUT 000528278500009
NAID 120006839453
Title Alternative Japanese guidelines for the treatment of hip fractures in the elderly
FullText URL 122_253.pdf
Author Noda, Tomoyuki| Ozaki, Toshifumi|
Publication Title 岡山医学会雑誌
Published Date 2010-12-01
Volume volume122
Issue issue3
Start Page 253
End Page 257
ISSN 0030-1558
language 日本語
Copyright Holders Copyright (c) 2010 岡山医学会
File Version publisher
DOI 10.4044/joma.122.253
NAID 130004903185
JaLCDOI 10.18926/AMO/32853
FullText URL fulltext.pdf
Author Endo, Hirosuke| Noda, Tomoyuki| Mitani, Shigeru| Nakahara, Ryuichi| Tetsunaga, Tomonori| Kunisada, Toshiyuki| Ozaki, Toshifumi|
Abstract <p>Femoroacetabular impingement (FAI) is a condition characterized by the impingement of the femur and acetabulum. In Japan, this disorder has become recognized gradually. Here we report a rare case of surgically treated FAI, associated with an osseous protrusion on the acetabulum of a 30-year-old female. Plain computed tomography (CT) and reconstructive 3D-CT images clearly demonstrated an anterolateral bony protrusion. Hip arthroscopy showed no degeneration of the cartilage on either the femoral or acetabular side, but degeneration at the edge of labrum was observed in the region of the bony protrusion. The complete removal of the bony protrusion under hip arthroscopy was thus considered impracticable, and a small skin incision was therefore made anteriorly to approach the acetabulum. The Short-Form 36-Item Health Survey (SF-36) revealed improvement in all scores.</p>
Keywords femoroacetabular impingement bony protrusion Pincer type hip arthroscopy SF36
Amo Type Case Report
Published Date 2010-04
Publication Title Acta Medica Okayama
Volume volume64
Issue issue2
Publisher Okayama University Medical School
Start Page 149
End Page 154
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 20424671
Web of Science KeyUT 000276996900010
JaLCDOI 10.18926/AMO/30972
FullText URL fulltext.pdf
Author Endo, Hirosuke| Asaumi, Koji| Mitani, Shigeru| Noda, Tomoyuki| Minagawa, Hiroshi| Tetsunaga, Tomonori| Ozaki, Toshifumi|
Abstract <p>A minimally invasive plate osteosynthesis technique using a locking compression plate (LCP) has been used widely in trauma cases. Its advantages are that the MIPO technique does not interfere with the fracture site and thus provides improved biological healing, and that the LCP has excellent angular stability. Its use in bone lengthening, however, has not been established. In such cases, it is desirable to shorten the external skeletal fixation period as much as possible. Here, the MIPO technique using an LCP was applied to femoral distraction osteogenesis in an attempt to shorten the external skeletal fixation period. For femoral lengthening, the MIPO technique was performed in 2 stages. Orthofix external fixators (Orthofix, England) were used to insert screws from the anterolateral side rather than from the lateral side of the femur for bone lengthening. When sufficient callus formation was detected postoperatively at the site of bone lengthening, and the absence of infection was ensured, limb draping was performed, including a whole external fixator, and then the MIPO technique was applied with an LCP. In 3 cases (5 limbs), the average duration of external skeletal fixation was 134days, the average external-fixation index was 24days/cm, and the average consolidation index was 22days/cm. The MIPO technique using an LCP made it possible to shorten the external skeletal fixation-wearing period in femoral lengthening.</p>
Keywords femoral lengthening minimally invasive plate osteosynthesis locking compression plate external skeletal fixation external-fixation index
Amo Type Original Article
Published Date 2008-10
Publication Title Acta Medica Okayama
Volume volume62
Issue issue5
Publisher Okayama University Medical School
Start Page 333
End Page 339
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 18985094
Web of Science KeyUT 000260391300008
Author Okada, Yoshiki| Abe, Nobuhiro| Hisamori, Noriyuki| Kaneeda, Toshiaki| Moriyama, Shigeaki| Ohmori, Hitoshi| Mizutani, Masayoshi| Yanai, Hiroyuki| Nakashima, Yoshio| Yokoyama, Yusuke| Ozaki, Toshifumi|
Published Date 2017-02
Publication Title Acta Medica Okayama
Volume volume71
Issue issue1
Content Type Journal Article
JaLCDOI 10.18926/AMO/54825
JaLCDOI 10.18926/AMO/47262
FullText URL 65_6_369.pdf
Author Terada, Chuji| Yoshida, Aki| Nasu, Yoshihisa| Mori, Shuji| Tomono, Yasuko| Tanaka, Masato| Takahashi, Hideo K.| Nishibori, Masahiro| Ozaki, Toshifumi| Nishida, Keiichiro|
Abstract We investigated the expression and localization of high-mobility group box chromosomal protein-1 (HMGB-1) in human osteoarthritic (OA) cartilage in relation to the histopathological grade of cartilage destruction, and examined the role of HMGB-1 in the regulation of proinflammatory cytokine expression in chondrocytes. An immunohistochemical study demonstrated that total HMGB-1-positive cell ratios increase as the Osteoarthritis Research Society International (OARSI) histological grade increased. The population of cytoplasmic HMGB-1-positive chondrocytes was especially increased in the deep layers of higher-grade cartilage. The ratios and localization of receptors for advanced glycation end products (RAGE) expression by chondrocytes in Grade 2, 3, and 4 were significantly higher than those in Grade 1. In vitro stimulation with IL-1β, but not TNFα, significantly upregulated the expression of HMGB-1 mRNA by human OA chondrocytes. Both IL-1β and TNFα promoted the translocation of HMGB-1 from nuclei to cytoplasm. IL-1β and TNFα secretions were stimulated at higher levels of HMGB-1. The results of our study suggest the involvement of HMGB-1 in the pathogenesis of cartilage destruction in OA.
Keywords HMGB-1 RAGE chondrocyte osteoarthritis cartilage
Amo Type Original Article
Published Date 2011-12
Publication Title Acta Medica Okayama
Volume volume65
Issue issue6
Publisher Okayama University Medical School
Start Page 369
End Page 377
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2011 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 22189477
Web of Science KeyUT 000298516900003
JaLCDOI 10.18926/AMO/46634
FullText URL 65_3_211.pdf
Author Nakanishi, Kazuo| Yamane, Kentarou| Tanaka, Masato| Misawa, Haruo| Saiga, Kenta| Ozaki, Toshifumi|
Abstract Here we report a case of surgery for kyphosis of the thoracolumbar spine in an elderly patient, in whom surgery was performed because the patient had developed intractable digestive symptoms. The case was that of a 76-year-old female with complaints of back pain and dysphagia. When videofluoroscopic examination (VF) of swallowing was performed in the cardia of the stomach, images that indicated stagnation and the reflux of food were observed. It was easier for the patient to swallow food in the extension position. We performed corrective fusion of the posterior spine. After the surgery, the kyphosis angle was improved to 27°, the patient's back pain was alleviated, and it became easier for the patient to swallow food. VF also showed that the patient's difficulties with the passage of food had improved. We believe that surgery is a good treatment option for cases of kyphosis with digestive symptoms and deteriorating activities of daily living (ADL), even in the absence of pain and paralysis. VF is also useful for performing evaluations before and after surgery.
Keywords kyphosis dysphagia videofluoroscopic examination of swallowing (VF) fusion
Amo Type Case Report
Published Date 2011-06
Publication Title Acta Medica Okayama
Volume volume65
Issue issue3
Publisher Okayama University Medical School
Start Page 211
End Page 214
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2011 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 21709720
Web of Science KeyUT 000292017500009
JaLCDOI 10.18926/AMO/32889
FullText URL fulltext.pdf
Author Nakanishi, Kazuo| Tanaka, Masato| Sugimoto, Yoshihisa| Ozaki, Toshifumi|
Abstract <p>We performed fixation using laminar screws in 2 patients in whom lateral mass screws, pedicle screws or transarticular screws could not be inserted. One was a 56-year-old woman who had anterior atlantoaxial subluxation (AAS). When a guide wire was inserted using an imaging guide, the hole bled massively. We thought the re-insertion of a guide wire or screw would thus increase the risk of vascular injury, so we used laminar screws. The other case was an 18-year-old man who had a hangman fracture. Preoperative magnetic resonance angiography showed occlusion of the left vertebral artery. A laminar screw was inserted into the patent side (i.e., the right side of C2). Cervical pedicle screws are the most biomechanically stable screws. However, their use carries a high risk of neurovascular complications during screw insertion, because the cervical pedicle is small and is adjacent laterally to the vertebral artery, medially to the spinal cord, and vertically to the nerve roots. Lateral mass screws are also reported to involve a risk of neurovascular injuries. The laminar screw method was thus thought to be useful, since arterial injuries could thus be avoided and it could also be used as a salvage modality for the previous misinsertion.</p>
Keywords laminar screw neurovascular complications lateral mass screw pedicle screw transarticular
Amo Type Case Report
Published Date 2007-04
Publication Title Acta Medica Okayama
Volume volume61
Issue issue2
Publisher Okayama University Medical School
Start Page 115
End Page 119
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 17471313
Web of Science KeyUT 000245875600010
JaLCDOI 10.18926/AMO/31849
FullText URL fulltext.pdf
Author Tanaka, Masato| Nakahara, Shinnosuke| Ito, Yasuo| Kunisada, Toshiyuki| Misawa, Haruo| Koshimune, Koichiro| Ozaki, Toshifumi|
Abstract <p>Surgical treatment of metastatic spinal cord compression is controversial. The purpose of this study was to investigate the effectiveness of our current surgical treatments and the use of spinal instrumentation. In this retrospective study covering the years between 1990 and 2006, 100 patients with spinal metastases which were secondary to various cancers underwent posterior and/or anterior decompression with spinal stabilization for the purposes of reduction of pain, and/or to help correct or improve neurological deficits. The group was made up of 60 men and 40 women whose ages ranged from 16 to 83 years (average of 60 years), and the average follow-up period was 14 months. The effect of treatment upon pain relief and neural deficits was assessed, and the cumulative survival rate was calculated by the Kaplan-Meier method. The average surgical time was 185min. This was calculated based on the following times, listed here with the surgery type:178min for posterior surgery;245min for anterior surgery;465 min for combined surgery;and 475min for total en bloc spondylectomy. Average blood loss during surgery was 1,630 ml for posterior surgery, 1,760 ml for anterior surgery, 1,930 ml for combined surgery, and 3,640 ml for total en bloc spondylectomy. Preoperative pain and paralysis were improved by 88% and 53%, respectively. In regards to surgical complications, postoperative epidural hematoma was observed in 2 patients, and instrumentation-related infection was observed in 1. Only 2 patients died within 2 months of surgery. In conclusion, posterior and/or anterior decompression with spinal stabilization is a safe and effective treatment for patients with spinal metastases, and can improve their quality of life.</p>
Keywords spinal metastasis spinal surgery instrumentation
Amo Type Original Article
Published Date 2009-06
Publication Title Acta Medica Okayama
Volume volume63
Issue issue3
Publisher Okayama University Medical School
Start Page 145
End Page 150
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 19571901
Web of Science KeyUT 000267388200004
JaLCDOI 10.18926/AMO/31823
FullText URL fulltext.pdf
Author Tanaka, Masato| Nakanishi, Kazuo| Sugimoto, Yoshihisa| Misawa, Haruo| Takigawa, Tomoyuki| Nishida, Keiichiro| Ozaki, Toshifumi|
Abstract <p>Scoliosis is a common clinical manifestation of Rett syndrome, a neurodevelopmental disorder that almost exclusively affects females. The spinal curve in patients with Rett syndrome is typically a long C curve of a neuromuscular type. As the onset of the scoliosis is very early and shows rapid progression, early surgical intervention has been recommended to prevent a life-threatening collapsing spine syndrome. However, there are high perioperative risks in Rett syndrome patients who undergo spinal surgery, such as neurological compromise and respiratory dysfunction due to rigid spinal curve. We herein report the surgical result of treating severe rapid progressive thoracic scoliosis in a 16-year-old girl with Rett syndrome. Posterior segmental pedicle screw fixation was performed from T1 to L3 using a computer-assisted technique. Post-operative radiography demonstrated a good correction of the curve in both the sagittal and coronal alignment. There were no postoperative complications such as neurological compromise. The patient had maintained satisfactory spinal balance as of the 3-year follow-up examination.</p>
Keywords Rett syndrome scoliosis computer navigation-assisted surgery segmental pedicle screw fixation
Amo Type Case Report
Published Date 2009-12
Publication Title Acta Medica Okayama
Volume volume63
Issue issue6
Publisher Okayama University Medical School
Start Page 373
End Page 377
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 20035294
Web of Science KeyUT 000273145900009
JaLCDOI 10.18926/AMO/30758
FullText URL fulltext.pdf
Author Tanaka, Masato| Nakahara, Shinnosuke| Ito, Yauo| Nakanishi, Kazuo| Sugimoto, Yoshihisa| Ikuma, Hisanori| Ozaki, Toshifumi|
Abstract <p>The purpose of this study was to investigate the surgical outcomes and to determine indicators of the necessity of surgical intervention. Twelve consecutive patients harboring symptomatic sacral perineural cysts were treated between 1995 and 2003. All patients were assessed for neurological deficits and pain by neurological examination. Magnetic resonance of imaging, computerized tomography, and myelography were performed to detect signs of delayed filling of the cysts. We performed a release of the valve and imbrication of the sacral cysts with laminectomies in 8 cases or recapping laminectomies in 4 cases. After surgery, symptoms improved in 10 (83%) of 12 patients, with an average follow-up of 27 months. Ten patients had sacral perineural cysts with signs of positive filling defect. Two (17%) of 12 patients experienced no significant improvement. In one of these patients, the filling defect was negative. In conclusion, a positive filling defect may become an indicator of good treatment outcomes.</p>
Keywords Tarlov cyst sacral perineural cyst meningeal cyst meningeal diverticulum sacral radiculopathy
Amo Type Article
Published Date 2006-02
Publication Title Acta Medica Okayama
Volume volume60
Issue issue1
Publisher Okayama University Medical School
Start Page 65
End Page 70
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 16508691
Web of Science KeyUT 000235538900008
Author Tanaka, Masato| Sugimoto, Yoshihisa| Misawa, Haruo| Takahata, Tomohiro| Ozaki, Toshifumi|
Published Date 2010-04-01
Publication Title 岡山医学会雑誌
Volume volume122
Issue issue1
Content Type Journal Article
JaLCDOI 10.18926/AMO/52008
FullText URL 67_6_351.pdf
Author Yokoyama, Yusuke| Abe, Nobuhiro| Fujiwara, Kazuo| Suzuki, Masahiko| Nakajima, Yoshikazu| Sugita, Naohiko| Mitsuishi, Mamoru| Nakashima, Yoshio| Ozaki, Toshifumi|
Abstract A computer-assisted navigation system to be used for total knee arthroplasties (TKAs) was reported to improve the accuracy of bone resection and result in precise implant placement, but the concomitant surgical invasion and time consumption are clinical problems. We developed a computed tomography (CT)-based navigation system (NNS) to be used for minimally invasive TKA. It requires only the reference points from a small limited area of the medial femoral condyle and proximal tibia through a skin incision to obtain optical images. Here we evaluated the usefulness and accuracy of the NNS in comparison with the commercially available BrainLAB image-free navigation system (BLS). In a clinical experiment, the registration times obtained with the NNS tended to be shorter than those obtained with the BLS, but not significantly so. The NNS group tended to be in the extended position in the sagittal plane of the distal femur within 3 degrees, and the BLS group showed rather flexed deviation in the sagittal plane of the anterior femur.
Keywords total knee arthroplasty navigation system minimally invasive surgery
Amo Type Original Article
Published Date 2013-12
Publication Title Acta Medica Okayama
Volume volume67
Issue issue6
Publisher Okayama University Medical School
Start Page 351
End Page 358
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2013 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 24356719
Web of Science KeyUT 000328915700003
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/52515