Author Ueda, Kazuhiro|
Published Date 2015-12-30
Publication Title Bulletin of Higher Education Okayama University
Volume volume11
Content Type Departmental Bulletin Paper
Author Ueda, Kazuhiro|
Published Date 2014-12
Publication Title 大学教育研究紀要
Volume volume10
Content Type Departmental Bulletin Paper
Author Ueda, Kazuhiro|
Published Date 2013-12-30
Publication Title 大学教育研究紀要
Volume volume9
Content Type Departmental Bulletin Paper
Author Ueda, Kazuhiro|
Published Date 2012-12-31
Publication Title 大学教育研究紀要
Volume volume8
Content Type Departmental Bulletin Paper
JaLCDOI 10.18926/AMO/32842
FullText URL fulltext.pdf
Author Kato, Yasuhiro| Mawatari, Hideo| Nishimura, Shin-Ichiro| Sakura, Nobuo| Ueda, Kazuhiro|
Abstract <p>Several specific assays have been developed for the measurement of etoposide in biological fluids. As large samples are required for high sensitivity, these systems are not appropriate for a pediatric practice. In the present study, however, an improved method for the determination of serum levels of the anticancer drug etoposide was developed, using high-performance liquid chromatography with fixed-wavelength ultraviolet detection. Etoposide was extracted from serum using dichloromethane. The efficiency of extraction from serum was 85.7 +/- 7.7% for etoposide and 81.1 +/- 8.4% for diphenylhydantoin, the internal standard. The serum concentrations of etoposide were measured in 0.2-ml serum samples. The lower limit of detection was 50 ng/ml. Each measurement was completed within 5 min. The linear quantitation range for etoposide was 0.05-50 microg/ml. This assay presents an alternative method for routine measurement of serum levels of etoposide in the pediatric oncology setting.</p>
Keywords etoposide serum concentration reversed-phase high-performance liquid chromatography
Amo Type Article
Published Date 2003-02
Publication Title Acta Medica Okayama
Volume volume57
Issue issue1
Publisher Okayama University Medical School
Start Page 21
End Page 24
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12765220
Web of Science KeyUT 000181198200003