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Author Hatipoglu, Omer F.| Miyoshi, Toru| Yonezawa, Tomoko| Kondo, Megumi| Amioka, Naofumi| Yoshida, Masashi| Akagi, Satoshi| Nakamura, Kazufumi| Hirohata, Satoshi| Ito, Hiroshi|
Keywords Aneurysm Aortic diseases
Published Date 2020-04-22
Publication Title Scientific Reports
Volume volume10
Issue issue1
Publisher Nature
Start Page 6869
ISSN 2045-2322
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
Copyright Holders © The Author(s) 2020
File Version publisher
PubMed ID 32321956
DOI 10.1038/s41598-020-63824-9
Web of Science KeyUT 000560329100004
Related Url isVersionOf https://doi.org/10.1038/s41598-020-63824-9
JaLCDOI 10.18926/AMO/31831
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Author Hatipoglu, Omer Faruk| Hirohata, Satoshi| Yaykasli, Kursat Oguz| Cilek, Mehmet Zeynel| Demircan, Kadir| Shinohata, Ryoko| Yonezawa, Tomoko| Oohashi, Toshitaka| Kusachi, Shozo| Ninomiya, Yoshifumi|
Abstract <p>ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1) is an inflammatory-induced gene. We have previously reported that ADAMTS1 was strongly but transiently expressed in the infarcted heart. In this study, we investigated whether a 3'-untranslated region (UTR) affects the mRNA stability of this gene. When stimulated with tissue necrosis factor (TNF)-alpha, the expression level of ADAMTS1 mRNA rapidly increased, but the induction of ADAMTS1 mRNA peaked at 6h after stimulation, after which the expression levels of ADAMTS1 mRNA decreased. The 3'-UTR ADAMTS1 mRNA contains multiple adenine and uridine-rich elements, suggesting that the 3'-UTR may regulate gene stability. The addition of actinomycin D, an RNA synthesis inhibitor, demonstrated the decay of induced ADAMTS1 mRNA by TNF-alpha. Furthermore, a region containing multiple AUUUA motifs within the ADAMTS1 3'-UTR destabilized transfected Enhanced Green Fluorescence Protein (EGFP) mRNA expression. These results demonstrated that the ADAMTS1 3'-UTR may regulate the expression of ADAMTS1 mRNA.</p>
Keywords ADAMTS1 gene regulation metalloproteinase
Amo Type Original Article
Published Date 2009-04
Publication Title Acta Medica Okayama
Volume volume63
Issue issue2
Publisher Okayama University Medical School
Start Page 79
End Page 85
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 19404339
Web of Science KeyUT 000265457600002
Author Demircan, Kadir| Hirohata, Satoshi| Nishida, Keiichiro| Hatipoglu, Omer F.| Oohashi, Toshitaka| Yonezawa, Tomoko| Apte, Suneel S.| Ninomiya, Yoshifumi|
Published Date 2005-5
Publication Title Arthritis & Rheumatism
Volume volume52
Issue issue5
Content Type Journal Article
Author 米澤 朋子|
Published Date 2005-05-20
Publication Title 岡山医学会雑誌
Volume volume117
Issue issue1
Content Type Journal Article
Author Yonezawa, Tomoko|
Published Date 2003-12-31
Publication Title
Content Type Thesis or Dissertation