Author 中村 寿美江| 石湯 和子| 谷本 悦子|
Published Date 1986-07
Publication Title 環境病態研報告
Volume volume57
Content Type Others
Author Institute for Environmental Medicine, Okayama University Medical Scool|
Published Date 1988-08
Publication Title 環境病態研報告
Volume volume59
Content Type Others
Author Institute for Environmental Medicine, Okayama University Medical Scool|
Published Date 1989-07
Publication Title 環境病態研報告
Volume volume60
Content Type Others
JaLCDOI 10.18926/19755
Title Alternative 気管支喘息および慢性気管支炎患者における抗ヒトIgEによる好塩基球からのヒスタミン遊離
FullText URL 059_031_036.pdf
Author Tanizaki, Yoshiro| Sudo, Michiyasu| Kitani, Hikaru| Araki, Hiroyuki| Tsuji, Mitsuaki| Takahashi, Kiyoshi| Kimura, Ikuro|
Abstract Histamine release from basophils induced by anti-IgE was studied in 8 patients with chronic bronchitis and 50 patients with bronchial asthma by analyzing doseresponse curves. As the result, there were no significant differences in maximum percent histamine release from basophils among three groups of healthy subjects (24.7± 14.2%), patients with chronic bronchitis (27.7±22.1%) and those with bronchial asthma (28.4±17.0%). In the patients with bronchial asthma, the maximum percent histamine release was higher in accordance with higher serum IgE levels, and low maximum percent release was observed in patients with intrinsic asthma (14.1±7.2%). Study of dose-response curves of anti-IgE-induced histamine release showed that a negative slope from E(2) to E(1) was observed in both healthy subjects and patients with chronic bronchitis. The majority of asthmatics with serum IgE levels of 501IU/ml or over showed a positive slope from E(2) to E(1).
Abstract Alternative 気管支喘息50例,慢性気管支炎8例を対象に,抗ヒトIgE添加時の好塩基球からのヒスタミン遊離を全血法により行ない,その臨床的評価について検討を加えた。抗ヒトIgE海底時のMax % histamine releaseの平均は,健康人24.7±14.2%,慢性気管支炎27.7±22.1%,気管支喘息28.4±17.0%であり,3者間に有意の差はみられなかった。すなわち,抗ヒトIgE添加により健康人や慢性気管支炎患者の好塩基球からも有意のヒスタミン遊離が見られた。気管支喘息のなかでは,内因性喘息症例においてヒスタミン遊離(14.1±7.2%)の低い傾向が見られた。Dose-response curveの検討では,健康人,慢性気管支炎症例では全例E(2)からE(1)へかけてのnegative slopeを示した。気管支喘息症例では,血清IgE値500IU/ml以下の症例ではnegative slopeを示す症例が多く,一方501IU/ml以上の症例ではpositive slopeを示す症例がより多く見られた。
Keywords Basophil response (好塩基球の反応性) Anti-lgE (抗ヒトIgE) Histamine release (ヒスタミン遊離) chronic bronchitis (慢性気管支炎) bronchial asthma (気管支喘息)
Publication Title 環境病態研報告
Published Date 1988-08
Volume volume59
Start Page 31
End Page 36
ISSN 09133771
language 英語
File Version publisher
NAID 120002309114
JaLCDOI 10.18926/11579
Title Alternative 気管支喘息患者末梢血好塩基球の抗ヒトIgEおよびカルシウムイオノフォァA23187に対する反応性について
FullText URL 061_016_022.pdf
Author Tanizaki, Yoshiro| Sudo, Michiyasu| Kitani, Hikaru| Kawauchi, Kazuhisa| Mifune, Takashi| Okuda, Hiroyuki| Komagoe, Haruki| Tada, Shinya| Takahashi, Kiyoshi| Kimura, Ikuro|
Abstract The release of histamine from basophils induced by anti-lgE and calcium ionophore A 23187 ( Cal) was examined in 27 patients with bronchial asthma and 7 healthy subjects, using a whole blood method. (1)The release of histamine induced by anti-IgE and CaI was significantly higher in atopic asthmatics than in non-atopic cases. (2) The histamine release with anti-lgE and CaI was significantly lower in cases with long-term steroid therapy compared to the release in cases without steroid therapy. (3) The release with anti-lgE and CaI was increased in cases with age of 0-39 years and in cases wpth age at onset of 0-39 years. (4) Anti-IgE induced release correlated to a certain extent with the release induced by CaI. These results show that basophil reactivity as expressed by histamine release changes under different conditions.
Abstract Alternative 気管支喘息27例および健康人7名を対象に,抗ヒトIgEおよびカルシウムイオノフォァA23187(CaI)刺激時の末梢血好塩基球からのヒスタミン遊離について検討を加えた。(1)抗ヒトIgEおよびCaI刺激時の好塩基球からのヒスタミン遊離は,非アトピー型喘息に比べ,アトピー型喘息において有意に高い値を示した。(2)これらの刺激物質によるヒスタミン遊離は,非使用例に比べ,ステロイド依存性喘息において低い傾向がみられた。また,年齢では39才以下,発症年齢では39才以下でヒズタミン遊離がより高い傾向がみられた。(3)血清IgE値の高い症例の好塩基球は,低い症例に比べより多くのヒスタミンを遊離する傾向がみられた。(4)全般的には,抗ヒトIgE刺激によるヒスタミン遊離とCaI刺激時の遊離との間にはある程度の相関がみられた。以上の結果より,好塩基球の反応性はいろいろの条件下でかなり異なることが示唆された。
Keywords Histamine release (ヒスタミン遊離) Basophils (好塩基球) Anti-lgE (抗ヒトIgE) Calcium ionophore A 23187 (カルシウムイオノフォァA23187) Bronchial asthma (気管支喘息)
Publication Title 環境病態研報告
Published Date 1990-09
Volume volume61
Start Page 16
End Page 22
ISSN 0913-3771
language 英語
File Version publisher
NAID 120002313673
JaLCDOI 10.18926/11685
Title Alternative 日本に於ける二酸化炭素泉療法の現況
FullText URL 061_068_072.pdf
Author Komoto, Yoshiaki| Soda, Mitsuhiro| Hirai, Shunichi| Morisue, Shinhachi| Suzuka, Ichio| Yorozu, Hidenori|
Abstract Natural CO(2) springs have been scarcely utilized in Japan, whereas artificial CO(2) bathing has become popular by the introduction of CO(2) tablets and other apparatus for CO(2) water supply. Artificial CO(2) bathing is indicated for degenerative connective tissue disorders such as scleroderma, common stiff and painful shoulders, chronic joint pain, lumbago, and trophic changes due to insufficient peripheral circulation by virtue of the increase of tissue pO(2) and the improvement of tissue perfusion. Artificial CO(2) bathing promotes recovery from fatigue and is beneficial in women whose health has been adversely affected by cold.
Abstract Alternative 日本では,天然の二酸化炭素泉は殆ど利用されていない。一方,CO(2)錠剤,CO(2)給水装置が開発されて人工二酸化炭素泉がよく用いられるようになってきた。皮下組織の酸素分圧の上昇と組織循環の改善という好ましい生理作用により,結合織の退行性変化による慢性障害,すなわち強皮症,関節症,慢性関節痛,腰痛症,および末梢循環障害に伴う組織の栄養障害に適応がある。また,疲労回復を促進し,いわゆる冷え症に好ましく作用する。
Keywords CO(2) -balneotherapy Artificial CO(2) -bathing Tissue pO(2)
Publication Title 環境病態研報告
Published Date 1990-09
Volume volume61
Start Page 68
End Page 72
ISSN 0913-3771
language 英語
File Version publisher
NAID 120002313982
JaLCDOI 10.18926/11668
Title Alternative カンジダ特異的IgG(4)抗体と気管支喘息
FullText URL 062_001_004.pdf
Author Tanizaki, Yoshiro| Kitani, Hikaru| Okazaki, Morihiro| Mifune, Takashi| Mitsunobu, Fumihiro| Takatori, Akimasa| Okuda, Hiroyuki| Harada, Hideo|
Abstract Serum levels of Candida-specific IgG(4) antibodies were examined in 66 patients with bronchial asthma, relating to patient age and asthma severity. 1. The levels of Candida-specific IgG(4) antibodies were the highest in patients with 60+years of age, compared to the levels in cases with 0-39 and 40-59 years of age. 2. In cases with 40-59 years of age, the levels of Candida-specific IgG(4) were significantly higher in cases with long-term steroid therapy (severe intractable asthma) than in cases without steroid regimen. The results suggest that increased levels of Candida-specific IgG(4) were observed in relation to patient age and asthma severity.
Abstract Alternative 66例の気管支喘息症例を対象に,血清中カンジダ特異的lgG(4)抗体を測定し,その血中レベルと年齢および喘息の重症度との関連について検討を加えた。1.血清カンジダ特異的IgG(4)値は,0~39才,40~59才の年齢層にくらべ,60才以上の年齢層において高く,年齢によりその値が変動することが 示唆された。2.40~59才の年齢層では,血清カンジダ特異的IgG(4)は,ステロイド非使用例にくらべ,ステロイド依存性重症難治性喘息症例で有意の高値を示し,この年齢層では,カンジダ特異的IgG(4)抗体がその発症病態に関連している可能性が示唆された。以上,カンジダ特異的IgG(4)抗体の上昇は,高年齢層の症例(60才以上)では全般的に,また40~59才の年齢層で重症型喘息症例において観察されることが明らかにされた。
Keywords Candida (カンジダ) Sepcific IgG(4) (特異的IgG(4)) Bronchial asthma (気管支喘息) Aging (加齢) Asthma severity (喘息重症度)
Publication Title 環境病態研報告
Published Date 1991-08
Volume volume62
Start Page 1
End Page 4
ISSN 0913-3771
language 英語
File Version publisher
NAID 120002313495
JaLCDOI 10.18926/19842
Title Alternative ステロイド依存性重症難治性喘息に対する温泉療法の遠隔効果
FullText URL 061_001_006.pdf
Author Tanazaki, Yoshiro| Sudo, Michiyasu| Kitani, Hikaru| Kawauchi, Kazuhisa| Mifune, Takashi| Okuda, Hiroyuki| Tada, Shinya| Takahashi, Kiyoshi| Kimura, Ikuro|
Abstract The immediate effects (IEs) and distant effects (DEs) of spa therapy were observed in 67 patients with steroid- dependent intractable asthma (SDIA). (1) The IEs of spa therapy evaluated one week after spa therapy were considerably high, and the efficacy rate was 61.5% in type Ⅰa, 82.7% in type Ⅰb, and 83.4% in type Ⅱ asthmatics. (2) The DEs of spa therapy on SDIA were also observed. The efficacy rate was 50.2% in type Ⅰa, 54.3% in type Ⅰb and 63.7% in type Ⅱ asthmatics. (3) The DEs of spa therapy were different between cases with and without maintenance therapy (MT). The efficacy rate of DEs was generally high (72.8~91.7%) in cases with MT, and low (16.7~40.0%) in cases without MT. These results show that MT is very important to keep the IEs of spa therapy high for a long time.
Abstract Alternative 67例のステロイド依存性重症難治性喘息を対象に,温泉療法を行い,その即時的効果(1週間後)および遠隔効果(1年後),さらには,温泉療法後の継続療法の影響などについて検討を加えた。1.温泉療法1週間後の即時的効果は比較的高く,その有効率は,Ⅰa.気管支攣縮型で61.5%,Ⅰb.気管支攣縮+過分泌型で82.7%,Ⅱ.細気管支閉塞型で83.4%であった。2.温泉療法の遠隔効果では,その有効率は,Ⅰa型で50.2%,Ⅰb型で54.1%,Ⅱ型で63.7%であった。3.温泉療法の遠隔効果は,その後の継続療法の有無によりかなり異なった結果であった。すなわち,継続療法を行った症例の有効率は,72.8~91.7%と高く,一方,継続療法を行わなかった症例の有効率は,16.7~40.0%の間にあった。これらの結果から,温泉療法の即時的効果を保つためには,その後の継続療法が極めて重要であることが示唆された。
Keywords lmmediate effects (即時的効果) Distant effects (遠隔効果) Maintenance therapy (継続療法) Spa therapy (温泉療法) Steroid-dependent intractable asthma (ステロイド依存性重症難治性喘息)
Publication Title 環境病態研報告
Published Date 1990-09
Volume volume61
Start Page 1
End Page 6
ISSN 09133771
language 英語
File Version publisher
NAID 120002309169
JaLCDOI 10.18926/19758
Title Alternative Separation of paripheral leukocytes by counterflow centrifugation elutriation
FullText URL 059_037_041.pdf
Author Sudo, Michiyasu| Matsubara, Fumie| Araki, Hiroyuki| Kitani, Hikaru| Tanizaki, Yoshiro|
Abstract JE-6B rotor(Beckman)を用いてcounterflow centrifugation elutriationによる末梢血白血球の分離を試み,若干の検討を加えた。1.flow rateが低い場合はリンパ球が大部分を占めたが,flow rateの増加とともに頻度は減少した。2. flow rateの増加とともに,好塩基球の頻度が増加し,9-10ml/minでは頻度のみでなく細胞数の増加も認められた。各fractionにおけるヒスタミン含有量も同様の傾向が認められた。3.好中球のpeakはflow rate 13ml/minであり以後減少を示した。4.最終段階で好酸球が増加傾向を示しChamber内で最も高値を示した。以上の結果よりcounterflow centrifugation elutriationによる末梢血白血球細胞分離は,in vitroにおける実験に有用な手段である可能性が示唆された。
Abstract Alternative Separation of peripheral leukocytes was carried out in 5 healthy subjects by counterflow centrifugation elutriation with JE-6B rotor. 1. Number of lymphocytes was predominant at low flow rate (4.5ml/min) of counterflow centrifugation elutriation. 2. As flow rate was increased (9-10ml/min), the purity and number of basophils, and histamine content in the fraction were gradually increased. 3. The purity and number of neutrophils were gradually increased, and the purity was reached the peak at 13ml/min. 4. The purity and number of eosinophils were gradually increased at the late stage of the centrifugation elutriation. The results obtained here might suggest that cell separation by counterflow centrifugation elutriation is more useful for experiments, especially allergy testing, in vitro.
Keywords 逆流負荷遠心法 (Counterflow centrifugation elutriation) JE-6Bローター (JE-6B rotor) 細胞分離 (Cell separation) 好塩基球 (Basophils)
Publication Title 環境病態研報告
Published Date 1988-08
Volume volume59
Start Page 37
End Page 41
ISSN 09133771
language 日本語
File Version publisher
NAID 120002309189
JaLCDOI 10.18926/15065
Title Alternative The biochemical and biological activities of JUN.
FullText URL 062_071_075.pdf
Author Mitsunobu, Fumihiro| Kitani, Hikaru| Okazaki, Morihiro| Mifune, Takashi| Asaumi, Noboru| Tanizaki, Yoshiro|
Abstract c-junはニワトリの癌ウイルスから分離された癌遺伝子(v-jun)のproto-oncogeneであり,その遺伝子産物は細胞内転写促進因子AP-1の主成分である。v-jun,c-junのトランスフォーム能を検討するために,我々はv-jun,ニワトリc-jun遺伝子さらにJUN遺伝子の種々の組換え体を複製能を持つレトロウイルスベクター(RCAS)に挿入し,CEF細胞(ニワトリ胎児線維芽細胞)にトランスフェクションした。その結果4か所存在する両者の構造的相違のうち,アミノ末端近くの27アミノ酸の欠損と3' untranslated regionの欠損が十分なトランスフォーム能と転写促進能発現に必須であることが明らかとなった。これらの部分がどのように機能発現に関与しているかは,これからの研究を待たねばならない。
Abstract Alternative V-jun is the oncogene which was isolated from the avian transforming virus ASV17. C-jun is the proto-oncogene of v-jun. The product of the proto-oncogene, c-jun, is a major component of the AP-1 transcription complex. AP-1 regulates the transcription of several genes through its ability to bind specifically to the sequence TGACTCA and variations of this motif. In order to assess the transforming capability of c-jun protein, we have introduced v-jun, the chicken c-jun proto-oncogene and several mutants into a replication competent avian retroviral expression vector (RCAS). Each of those was expressed in CEF and assayed for transformation by focus formation. Analysis of the results reveals that deletion of a region of 27 amino acids near the amino terminus of c-jun and deletion of 3' untranslated sequences are critical in activating the full oncogenic potential of jun.
Keywords 癌遺伝子 (Oncogene) JUN遺伝子 (JUN gene) レトロウイルスベクター (Retrovirus Vector) トランスフェクション (Transfection) トランスフォーム (Transform)
Publication Title 環境病態研報告
Published Date 1991-08
Volume volume62
Start Page 71
End Page 75
ISSN 0913-3771
language 日本語
File Version publisher
NAID 120002307874
JaLCDOI 10.18926/11648
Title Alternative 種々の長さのスペーサーをもつハプテン化ボスファチジルエタノールアミンの新しい合成法
FullText URL 062_032_037.pdf
Author Ishimori, Yoshio| Yasuda, Tatuji|
Abstract The antigenicity of liposomes sensitized with haptenated phosphatidylethanolamine (PE) and the reactivity of the liposomes with complement depended on the length of the spacer between hapten and PE. To establish the optimal conditions for the assay, haptenated PE's with various length of spacers are required. In the previous method, hapten-spacer molecule was first synthesized to which PE was conjugated. Therefore, even different hapten molecules and different length of spacer molecules were used, every combination of hapten and spacer has to be synthesized. A new procedure for preparing hapten-spacer-PE was described here. We first prepared conjugates between PE and various length of spacer molecule, the terminal of which is an amino residue. These molecules react well with activated hapten molecules, giving a good yield of hapten-spacer-PE.
Abstract Alternative 人工脂質膜であるリボソームにハプテン化ホスファチジルエタノールアミン(PE)を挿入することで,リボソーム膜上での免疫反応の研究が進んでいる。いろいろな因子のなかでリボソーム表面とハプテン基の間のスペーサーの長さも重要な因子であることが判明してきた。このスペーサーの役割を研 究するためには汎用性のある合成法の開発が望まれている。これまでのハプテン基一スペーサー分子を結合する方法は種類の異なるハプテン基をもつ分子群を合成するには煩雑である。そこで種々のスペーサーをもつPEを先に合成することで種類の違うハプテン基をもち,異なるスペーサーをもつハプテン化脂質抗原の合成法を開発した。
Keywords Haptenated phosphatidylethanolamine (ハプテン化ホスファチジルエタノールアミン) Spacer (スペーサー) Liposomes (リポソーム)
Publication Title 環境病態研報告
Published Date 1991-08
Volume volume62
Start Page 32
End Page 37
ISSN 0913-3771
language 英語
File Version publisher
NAID 120002313629
JaLCDOI 10.18926/19710
Title Alternative Study on anti-allergic action of Terfenadine
FullText URL 058_005_011.pdf
Author Tanizaki, Yoshiro| Komagoe, Haruki| Sudo, Michiyasu| Kitani, Hikaru|
Abstract 選択的H(1)受容体拮抗薬であるTerfenadineの抗アレルギー作用,特にIgE系反応によるヒスタミン,およびCa ionophore A23187による好中球からのleukotrienes遊離に対する抑制作用を,ヒト末梢血白血球を用いて検討した。1.Terfenadineは,抗ヒトIgE刺激による健康人好塩基球および特異抗原刺激による気管支喘息患者好塩基球からのヒスタミン遊離に対して,全血法でのin vitro添加実験では有意の抑制作用を示さなかった。 2.洗浄白血球法によるin vitro添加実験では,Terfenadineは特異抗原および抗ヒトIgE刺激時の気管支喘息患者好塩基球からのヒスタミン,Ca ionophore A23187刺激時のleukotriene B(4)遊離に対して有意の抑制効果を示した。3.Terfenadineの内服によるin vivoの抑制実験では,IgE系反応による健康人および気管支喘息患者好塩基球からのヒスタミン遊離に対して有意の抑制効果は見られなかったが,特異抗原による皮膚反応に対しては明らかな抑制作用が観察された。以上より,Terfenadineは,組織肥満細胞および組織へと遊走してきた好塩基球に対してはある程度の抑制作用を有しているものと考えられた。
Abstract Alternative Anti-allergic action of terfenadine, one of the H(1) receptor inhibitors, was examined by human basophil histamine release induced by anti-IgE or a specific allergen (house dust), and the release of leukotriens from leucocytes stimulated by Ca ionophore A23187. 1. Terfenadine did not inhibit the release of histamine from basophils of healthy subjects induced by anti-IgE, and also of patients with bronchial asthma induced by house dust when the in vitro examination was performed by a whole blood method. 2. Histamine release from basophils of patients with bronchial asthma was significantly inhibited when washed leucocytes was preincubated with terfenadine in vitro. The release of LT B(4) from leucocytes was also inhibited. 3. In vivo examination of inhibitory action of terfenadine showed no effect on basophil histamine release of healthy subjects by anti-IgE and of asthmatic subjects by house dust, although skin test by house dust in these subjects was inhibited 60 min after administration in vivo. These results show that terfenadine inhibits the release of chemical mediators from tissue mast cells, and basophils migrating into local allergic sites.
Keywords 夕一フェナンジン (Terfenadine) lgE系反応 (IgE-mediated reaction) ヒスタミン (histamine) 好塩基球 (basophils)
Publication Title 環境病態研報告
Published Date 1987-08
Volume volume58
Start Page 5
End Page 11
ISSN 09133771
language 日本語
File Version publisher
NAID 120002309197
JaLCDOI 10.18926/19683
Title Alternative Studies on anti-allergic actions of tranilast (Rizaben®)
FullText URL 057_042_045.pdf
Author Tanizaki, Yoshiro| Komagoe, Haruki| Sudo, Michiyasu| Morinaga, Hiroshi| Ohtani, Jun| Kimura, Ikuro|
Abstract 抗アレルギー剤(脱顆粒抑制剤)の1つであるtranilastの肥満細胞の遊離機序に対する抑制作用について検討を加えた。1.抗原刺激時の肥満細胞の(45)Ca uptakeおよびヒスタミン遊離に対して,tranilastは有意の抑制作用を示したが,comp.48/80刺激時にはtranilastの(45)Ca uptake,ヒスタミン遊離に対する抑制作用はほとんどみられなかった。2.phosphatidylserine添加時には,(45)Ca uptakeに対するtranilastの抑制作用は減弱傾向を示し,この傾向はヒスタミン遊離に対する作用に比べより高度であった。3.抗原および抗ヒトIgEによる好塩基球からのヒスタミン遊離に対して,tranilastは明らかな抑制作用を示さなかった。
Abstract Alternative Tlanilast is clinically used for bronchial asthma as one of the prophylactic agents for asthma attacks. In this study, anti-allergic actions of tranilast were examined in (45)Ca uptake of and histamine release from target cells of IgE after stimulation with antigen, anti-IgE and camp. 48/80. 1. (45)Ca uptake of and histamine release from rat peritoneal mast cells stimulated by antigen were significantly inhibited by preincubation of the cells with tranilast. Inhibition by tranilast of increased (45)Ca uptake of mast cells by addition of phosphatidylserine were less, although inhibition of histamine release was not so affected by addition of phosphatidylserine. No significant inhibition by tranilast of (45)Ca uptake and histamine release was not observed when the cells were stimulated by camp. 48/80. 2. Tranilast showed any inhibitory effects on basophil histamine release induced by antigen and anti-IgE.
Keywords トラニラスト (tranilast) (45)Ca取り込み ((45)Ca uptake) ヒスタミン遊離 (histamine release) 肥満細胞 (mast cell) 好塩基球 (basophils)
Publication Title 環境病態研報告
Published Date 1986-07
Volume volume57
Start Page 42
End Page 45
ISSN 09133771
language 日本語
File Version publisher
NAID 120002308998
JaLCDOI 10.18926/19771
Title Alternative トランスフェリンを介する鉄の細胞内取り込み機序 ―特に肝細胞への取り込みにおける内皮細胞の関与について―
FullText URL 059_083_092.pdf
Author Irie, Seiji| Matsumoto, Shuji| Ochi, Koji| Takeda, Masahiko| Tanaka, Juntaro| Harada, Hideo| Mehdi, Tavassoli|
Abstract Transferrin (Tf) is thought to play a pivotal role in iron metabolism of various kinds of cells. Tf has specific receptors on the surface of the cells that require iron. Tf-receptor binding is followed by internalization through a system of coated pits and vesicles. The rapid decline of pH of these vesicles leads to the release and sequestration of iron by the cell. Apotransferrin-receptor complex returns to the cell surface, where under neutral pH conditions, apotransferrin is dissociated from the receptor. Recent advances in cellular and molecular biology, gene cloning and monoclonal antibody technique have elucidated many features of these processes at a molecular level. These advances are briefly reviewed, and particularly, our own observations concerning endothelial mediation of uptake of Tf by hepatocytes are discussed.
Abstract Alternative トランスフェリン(Tf)は鉄代謝において重要な役割を担っている。鉄を必要とする細胞の表面には,Tfの特異的受容体が存在し,Ffは受容体との結合に引き続いて,coated pitsとcoated vesiclesを介して細胞内に取り込まれる(internalization)。Vesicle内での急速なpHの低下にともない,鉄はTfから分離し,細胞内で分画される。一方,鉄を失ったTf(アポTf)は受容体と結合したまま細胞表面にもどり,中性のpHのもとで受容体から解離する。最近の細胞・分子生物学の進歩,遺伝子クローニング,ならびにモノクローナル抗体の開発により,分子レベルで鉄代謝経路が解明されつつある。今回,これまでの主要な知見をまとめ,特に,最近筆者らの研究により明らかとなった,肝の鉄代謝における内皮細胞の役割について考察した。
Keywords Iron uptake (鉄の細胞内取り込み) Transferrin (トランスフェリン) Receptor-Mediated Endocytosis Desialation (脱シアル化) Endothelium (内皮細胞)
Publication Title 環境病態研報告
Published Date 1988-08
Volume volume59
Start Page 83
End Page 92
ISSN 0913-3771
language 英語
File Version publisher
NAID 120002309113
Author Yasuda, Tatsuji|
Published Date 1991-08
Publication Title 環境病態研報告
Volume volume62
Content Type Others
JaLCDOI 10.18926/15041
Title Alternative Alcohol-induced pancreatitis
FullText URL 062_052_070.pdf
Author Harada, Hideo| Tanaka, Juntaro| Ochi, Koji| Matsumoto, Shuji| Seno, Toshinobu| lzushi, Michihiro| Tanizaki, Yoshiro|
Abstract 膵炎のうちもっとも頻度が高いアルコール性膵炎(AIP)の疫学,臨床像および発生機序に関する従来の知見を総括した。AIPの大多数は慢性膵炎である。通常は長期にわたる多量の飲酒を背景に発症するが,遺伝的素因および食事因子も重要な役割を演じる。発症初期には血中膵酵素の上昇をともなう腹痛が病像を支配するが,進展すると膵外分泌不全による消化吸収障害と膵内分泌不全による糖尿病が病像を支配するようになる。アルコール性慢性膵炎は非アルコール性慢性膵炎にくらべて確診時にすでに進展した症例が多く,合併症が多く,進行が早く,予後が悪い。死亡の主たる原因は癌の併発と糖尿病の合併症で,膵炎の急性増悪発作がこれにつぐ。併発する癌のなかでは膵癌よりもむしろ上部気道および上部消化管の癌が多い。発生機序としてはDuctal-Plug説とToxic-Metabolic説が有力であるが,最近は細 胞内膵酵素活性化説とFree Radical説も注目をあびている。
Abstract Alternative This paper is to review the literature on the epidemiology, clinical pictures and etiopathogenesis of alcohol-induced pancreatitis (AIP). The incidence of AIP has been increasing worldwide, paralleling the increase in alcohol consumption. AIP manifests itself following a longterm consumption of large amounts of alcohol. There is no known threshold value of alcohol consumption in terms of the risk of developing AIP, although the logarithm of the risk of developing AIP is lineally correlated with the amount of alcohol intake. Why some alcoholics develop pancreatitis whereas others with equal consumption of ethanol are spared remains to be explained. Therefore, two additional factors are considered to play important roles in developing AIP : genetic predisposition and diet. The majority of AIP IS chronic pancreatitis (AICP), although a minority can be acute pancreatitis (AIAP). AIAP shows somewhat higher morbidity and mortality than the common variety of acute pancreatitis. If recovered from an attack, AIAP shows morphological and functional restoration. AICP manifests itself with an acute attack of abdominal pain, insidious onset of abdominal pam, or a pain-free variety. An acute attack in AICP resemble that m AIAP ; often these two can be differentiated only by follow-up studies. AICP shows no morphological and functional restoration, and often shows progressive deterioration. Abdominal pain with elevated serum pancreatic enzymes is a predominant clinical picture m the early stage of AICP, whereas in the late stage symptoms and signs deriving from exocrine insufficiency (maldigestion) and endocrine insufficiency (pancreatic diabetes) begin to dominate the clinical pictures. AICP is in the more advanced stage and shows more complications than nonalcoholic chronic pancreatitis at the time of diagnosis. In addition, AICP shows more rapid progress and higher morbidity and mortality. The incidence of microangiopathy in pancreatic diabetes resemble that in primary diabetes, being higher in patients with a longer history of diabetes, those on insulin treatment and those under poorer control. Main causes of death are development of cancer in the upper respiratory and gastrointestinal tract and diabetic complications (hypoglycemic shock, renal failure, and intractable pneumonia), and acute attack of pancreatitis leads to death less frequently. Ductal-Plug theory and classical Toxic-Metabolic theory are most popular to explain the pathogenesis of AIP ; however, increasing evidence has been reported that oxygen free radicals and intracellular activation of zymogens by lysosomal enzymes may be involved in the pathogenesis.
Keywords アルコール性膵炎 (Alcohol-induced pancreatitis) 慢性膵炎 急性膵炎
Publication Title 環境病態研報告
Published Date 1991-08
Volume volume62
Start Page 52
End Page 70
ISSN 0913-3771
language 日本語
File Version publisher
NAID 120002307918
JaLCDOI 10.18926/15097
Title Alternative Possible participation of viral infection in the onset mechanisms of bronchial asthma
FullText URL 062_076_079.pdf
Author Mifune, Takashi| Mitunobu, Fumihiro| Okazaki, Morihiro| Kitani, Hikaru| Tanizaki, Yoshiro|
Abstract ウイルスなどの気道感染により,気管支喘息症状が発症したり,あるいは増悪したりすることが知られている。自験例は65歳の女性で,上気道炎症状に引き続いて,気管支喘息が発症した。臨床症状の軽快とともに気道過敏性・IgE値は低下し,感染による気道過敏性の亢進,IgE型アレルギー反応の機序が関与していると考えられた。これらの結果は喘息患者は気道感染(特にウイルス感染)の予防が必要であり,感染した場合には抗喘息剤とともに抗炎症剤の使用が有用である可能性を示している。
Abstract Alternative It is well known that respiratory infection induces asthma attacks. In this paper one case showing possible participation of viral infection in the onset mechanisms of bronchial asthma was reported. A 65-year-old woman was admitted to Misasa Hospital because of moderate asthma attacks. The attacks appeared after upper respiratory infection. Bronchial hyperactivity were increased when she admitted at the hospital, and decreased with the improvement of the asthma symptoms. It is suggested from her clinical course that repiratory infection (especially by virus) may affect the bronchial hyperactivity and IgE synthesis.
Keywords 気道感染 (Respiratory infection, Virus infection) 気道過敏性 (Bronchial hypersensitivity) lgEアレルギー反応 (Allergic reaction mediated by IgE)
Publication Title 環境病態研報告
Published Date 1991-08
Volume volume62
Start Page 76
End Page 79
ISSN 0913-3771
language 日本語
File Version publisher
NAID 120002307679
JaLCDOI 10.18926/11679
Title Alternative Clinical evaluation of new formula included oligopetide as a nitrogen source in surgical patients
Author Soda, Mitsuhiro| Yorozu, Hidenori| Morisue, Sinhachi| Hirai, Shunichi| Suzuka, lchio| Komoto, Yoshiaki| Kajitani, Nobuaki| Yoshizane, Ken| Naniwa, Hiroyuki| Teramoto, Shigeru|
Abstract 窒素源としてdi-,tri-peptideを含有した経腸栄養剤を外科患者に使用した。.この新しい経腸栄養剤は卵白を加水分解したもので,70%がoligopeptideであり,アミノ酸も10%含まれる。糖質はデキストリンで,脂肪の含有量は少なく浸透圧はエレメンタルダイエット(ED)より低い。この製剤を安定期および術後に使用した。アルブミン,rapid turnover proteinの回復がみられ,臓器蛋白の合成が得られた。術後例では3メチルヒスチジンの排泄量は減少せず,異化反応は抑制されなかった。投与量の不足が一つの原因と思われる。副作用の下痢はほとんどみられず,栄養改善効果もあり,有用性が認められた。
Abstract Alternative The clinical usefulness of new enteral formula which is contained di-and tri-peptides as a nitrogen source was evaluated in surgical patients. The enteral nutrition was introduced to stable patients who could not take food orally and postoperative ones who were undertaken upper GI surgery. Adverse effects such as diarrhea or abdomonal distension were not observed. V isceral proteins were improved after administration of this regimen and nutritional status was maintained in all patients. However, high urinary excretion of 3methylhistidine continued in great extent during postopertive period and breakdown of skeletal muscle was not prevented by this nutritional therapy. This might be due to insufficient intake of energy and nitrogen. This new product for enteral nutrition would be safe and useful as enteral nutrition.
Keywords 経腸栄養 (Enteral nutrition) エレメンタルダイエット (Elemental diet) オリゴペプチド (Oligopeptide) 外科栄養 (Surgical nutrition)
Publication Title 環境病態研報告
Published Date 1990-09
Volume volume61
Start Page 61
End Page 67
ISSN 0913-3771
language 日本語
File Version publisher
JaLCDOI 10.18926/11696
Title Alternative Thermological study on the coldness in women
FullText URL 061_046_054.pdf
Author Takatori, Akimasa| Okuda, Hiroyuki| Sekiba, Kaoru| Tanizaki, Yosiro|
Abstract サーモグラフィを用いた冷え症の診断基準を作ることを目的として,冷え症患者(30名)の体表面温度分布を体表面を11カ所に分けて中間温環境下でサーモトレーサ6T66を1用いて測定し,対称群(11名)と比較した。同時にCMI調査と,患者の日常生活に関するアンケート調査を実施した。その結果1.冷え症群では四肢末端部,ことに手掌・足背・足底において最低温度が低く,局所の最高・最低温度間の温度較差が大きい傾向を示した。軀幹部では最高温度,最低温度,温度較差すべて両群間に大きな差はみられなかった。2.冷え症群の手掌,足背,足底の温度分布パターンは末端部ほど温度が低いという特徴的なパターンを多く示した。3.CMI調査では,冷え症群の中等症と軽症例の一部に強い神経症的傾向を認めたが,重症例では神経症的傾向は軽度であった。4.アンケート調査では脂っこいものよりあっさりしたものを好きであるとか,顔にのぼせを感じることがある,甘いものが好きであるという例が冷え症群に多かった。以上の結果からサーモグラフィを用いて中間温環境下で四肢末端の温度鈴布を測定することにより,冷え症を客観的に診断する可能性を得た。
Abstract Alternative Whole body surface temparature was measured in 41 women with a thermtracer 6T66. Except palm, sole and instep, there were no differences in surface temperature of other parts of the body among these cases. Surface temperature of palm, sole and instep were lower than other parts of the body and showed 4 characteristic distribution patterns on thermography. Thermography at palm, sole and instep is useful for the objective approach of the patients who complain of coldness in some part of their bodies.
Keywords 婦人の冷え症 (Coidness in women) サーモグラフィ (Thermography)
Publication Title 環境病態研報告
Published Date 1990-09
Volume volume61
Start Page 46
End Page 54
ISSN 0913-3771
language 日本語
File Version publisher
NAID 120002313877
JaLCDOI 10.18926/11662
Title Alternative Thermological study on the coldness women - Second report. The relationship between the change of atmospheric temperature and body surface temperature in women with complaints of coldness in limbs
FullText URL 062_016_022.pdf
Author Takatori, Akimasa| Okuda, Hiroshi| Sekiba, Kaoru| Tanizaki, Yoshiro|
Abstract 季節毎の婦人の冷え性患者における全身の皮膚表面温度分布について,サーモグラフィを用いて検討した。その結果以下のことが判明した。1)冷え性は外気温が1カ月の最高気温が20℃,最低気温が10℃,平均気温で15℃以下になると発症しやすくなる。2)外気温が前記の条件において,中間温環境下でサーモグラフィ上,軀幹部の最高温度と四肢部の最低温度の差が8℃以上ある症例では99%の確率で冷えを訴えることが明かとなった。
Abstract Alternative From January to December in 1990, whole body temperature was measured in 79 women by using a thermotracer 6T66, and was compared to the change of atmospheric temperature.The difference between the highest temperature of the body and the lowest temperature of the limbs was more than 8℃ in women with complaints of coldness of limbs. This difference was observed in 88% of the women complaining coldness when the average atmospheric temperature in a month was more than 15℃.
Keywords 婦人の冷え性 (Coldness in women) サーモグラフィ (Thermography) 気温の変化 (Change of atmospheric temperature)
Publication Title 環境病態研報告
Published Date 1991-08
Volume volume62
Start Page 16
End Page 22
ISSN 0913-3771
language 日本語
File Version publisher
NAID 120002313602