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ID 48098
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Study on the change of glomerular antigenicity in various renal diseases with anti-human renal monoclonal antibodies
Author
Mino, Yasuaki
Abstract
We produced 22 different kinds of monoclonal antibody (Mab) by immunizing mice with human GBM antigens. In these Mabs, Mab-G1 to G5 recognized only GBM in the glomerulas, Mab-E1 and E2 recognized only glomerular epithelial cells, and Mab-M1 to M4 recognized mainly mesangium. The reactions of these Mabs with known GBM antigens such as type IV collagen, fibronectin and laminin were negative by immunoblotting. Using Mab-G1, Mab-E1 and Mab-M1, changes in the antigenicity of antigens recognized by Mabs were examined on kidney sections from the patients with various renal diseases by the indirect immunofluorescence test. When Mab-G1 recognizing GBM was used, there was no particular change of anti-genicity in minimal change nephrotic syndrome (MCNS) and IgA nephropathy (IgA), whereas in membranous nephropathy (MN) thickened GBM was found to maintain anti-genicity and the region of deposits was observed as negative punched-out region. In type I and III of membranoproliferative glomerulonephritis (MPGN), GBM was observed only outside of subendothlial deposits without showing double contour. In type II MPGN, GBM showed a double linear pattern and antigenicity of GBM in regions of dense deposits was not detected. When Mab-E1 recognizing glomerular epithelial cells was used, there was no change of antigenicity in the renal diseases. Further, in crescentic glomerulone-phritis, the region of the cellular crescents was not stained, When Mab-M1 recognizing mesangium was used, extensive staining was observed in the increased mesangium in IgA, MPGN, and diabetic nephropathy. We feel that it is of significance in elucidating the pathogenesis of renal diseases to study the changes of glomerular antigenicity in diseased kidneys by using anti-human renal monoclonal antibodies.
Keywords
monoclonal antibody
human kidney antigen
GBM
mesangium
glomerular epithelial cell
Published Date
1990
Publication Title
日本腎臓学会誌
Publication Title Alternative
The Japanese Journal of Nephrology
Volume
volume32
Issue
issue4
Publisher
社団法人日本腎臓学会
Publisher Alternative
Japanese Society of Nephrology
Start Page
353
End Page
364
ISSN
0385-2385
NCID
AN10131749
Content Type
Journal Article
Official Url
http://joi.jlc.jst.go.jp/JST.Journalarchive/jpnjnephrol1959/32.353
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/6267
language
日本語
Copyright Holders
Copyright© 社団法人日本腎臓学会
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publisher
Refereed
True