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ID 48099
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Title Alternative
Assay of GBM antigen in urine and serum with an anti-human renal monoclonal antibody
Author
Mino, Yasuaki
Abstract
Using a monoclonal antibody (Mab-G3) recognizing glomerular basement membrane (GBM), we assayed GBM antigen (G3-Ag) in the urine and serum of renal disease patients by sandwich ELISA. The subjects included normal control (NOR), minimal change nephrotic syndrome (MCNS), IgA nephropathy (IgA), membranous nephropathy (MN), membranoproliferative glomerulonephritis (MPGN) and chronic renal failure (CRF). The urine and serum was used as the material. With urinary G3-Ag, there were no statistically significant differences among the NOR, MCNS, IgA, MN, MPGN and CRF groups. Although no correlation was observed with proteinuria, hematuria, serum creatinine, serum β2 microglobulin and urinary NAG, urinary G3-Ag showed a significant (p<0.05) increase in excretion in the group of progressive CRF patients with s-Cr more than 1.0 mg/dl/month as compared to the stationary CRF group with s-Cr<1.0 mg/dl/month. Serum G3-Ag showed lower values in almost all cases, and there were no significant differences among the renal disease groups. The above findings led us to believe that the assay of urinary G3-Ag was useful in determining the degree of GBM disorder. It was also presumed that assay of renal antigens in urine and serum with the respective anti-human renal monoclonal antibodies could be a new tool in diagnosing renal diseases.
Keywords
monoclonal antibody
sandwich ELISA
GBM antigen
renal disease
Published Date
1990
Publication Title
日本腎臓学会誌
Publication Title Alternative
The Japanese Journal of Nephrology
Volume
volume32
Issue
issue4
Publisher
社団法人日本腎臓学会
Publisher Alternative
Japanese Society of Nephrology
Start Page
365
End Page
372
ISSN
0385-2385
NCID
AN10131749
Content Type
Journal Article
Official Url
http://joi.jlc.jst.go.jp/JST.Journalarchive/jpnjnephrol1959/32.365
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/6267
language
日本語
Copyright Holders
Copyright© 社団法人日本腎臓学会
Refereed
True