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ID 46890
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Author
Miyake, Yoshiaki
Furumatsu, Takayuki Kakenhi
Kubota, Satoshi Kakenhi
Kawata, Kazumi
Ozaki, Toshifumi Kakenhi
Abstract
Anterior cruciate ligament (ACL)-to-bone interface serves to minimize the stress concentrations that would arise between two different tissues. Mechanical stretch plays an important role in maintaining cell-specific features by inducing CCN family 2/connective tissue growth factor (CCN2/CTGF). We previously reported that cyclic tensile strain (CTS) stimulates alpha 1(I) collagen (COL1A1) expression in human ACL-derived cells. However, the biological function and stress-related response of CCN2/CTGF were still unclear in ACL fibroblasts. In the present study, CCN2/CTGF was observed in ACL-to-bone interface, but was not in the midsubstance region by immunohistochemical analyses. CTS treatments induced higher increase of CCN2/CTGF expression and secretion in interface cells compared with midsubstance cells. COL1A1 expression was not influenced by CCN2/CTGF treatment in interface cells despite CCN2/CTGF stimulated COL1A1 expression in midsubstance cells. However, CCN2/CTGF stimulated the proliferation of interface cells. Our results suggest that distinct biological function of stretch-induced CCN2/CTGF might regulate region-specific phenotypes of ACL-derived cells.
Keywords
CCN2/CTGF
Anterior cruciate ligament
Cyclic tensile strain
Collagen
Ligament-to-bone interface
Published Date
2011-06-03
Publication Title
Biochemical and Biophysical Research Communications
Volume
volume409
Issue
issue2
Publisher
Academic Press Inc Elsevier Science
Start Page
247
End Page
252
ISSN
0006-291X
NCID
AA00564395
Content Type
Journal Article
language
英語
Copyright Holders
© 2011 Elsevier Inc. All rights reserved.
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Refereed
True
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