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ID 46906
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Author
Saiga, Kenta
Furumatsu, Takayuki Kakenhi
Yoshida, Aki
Masuda, Shin
Takihira, Shota
Ozaki, Toshifumi Kakenhi
Abstract
Basic fibroblast growth factor (bFGF) and growth and differentiation factor (GDF)-5 stimulate the healing of medial collateral ligament (MCL) injury. However, the effect of isolated and combined use of bFGF/GDF-5 remains still unclear. We investigated cellular proliferation and migration responding to bFGF/GDF-5 using rabbit MCL fibroblasts. Rabbit MCL injury was treated by bFGF and/or GDF-5 with peptide hydrogels. Gene expression and deposition of collagens in healing tissues were evaluated. bFGF/GDF-5 treatment additively enhanced cell proliferation and migration. bFGF/GDF-5 hydrogels stimulated Col1a1 expression without increasing Col3a1 expression. Combined use of bFGF/GDF-5 stimulated type I collagen deposition and the reorganization of fiber alignment, and induced better morphology of fibroblasts in healing MCLs. Our study indicates that combined use of bFGF/GDF-5 might enhance MCL healing by increasing proliferation and migration of MCL fibroblasts, and by regulating collagen synthesis and connective fiber alignment.
Keywords
Medial collateral ligament
bFGF
GDF-5
Hydrogel scaffold
Ligament healing
Published Date
2010-11-12
Publication Title
Biochemical and Biophysical Research Communications
Volume
volume402
Issue
issue2
Publisher
Academic Press Inc Elsevier Science
Start Page
329
End Page
334
ISSN
0006-291X
NCID
AA00564395
Content Type
Journal Article
language
英語
Copyright Holders
© 2010 Elsevier Inc. All rights reserved.
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Refereed
True
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