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ID 46907
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Author
Furumatsu, Takayuki Kakenhi
Hachioji, Motomi
Saiga, Kenta
Takata, Naoki
Yokoyama, Yusuke
Ozaki, Toshifumi Kakenhi
Abstract
Anterior cruciate ligament (ACL)-derived cells have a character different from medial collateral ligament (MCL)-derived cells. However, the critical difference between ACL and MCL is still unclear in their healing potential and cellular response. The objective of this study was to investigate the mesenchymal differentiation property of each ligament-derived cell. Both ligament-derived cells differentiated into adipogenic, osteogenic, and chondrogenic lineages. In chondrogenesis, ACL-derived cells had the higher chondrogenic property than MCL-derived cells. The chondrogenic marker genes, Sox9 and α1(II) collagen (Col2a1), were induced faster in ACL-derived pellets than in MCL-derived pellets. Sox9 expression preceded the increase of Col2a1 in both pellet-cultured cells. However, the expression level of Sox9 and a ligament/tendon transcription factor Scleraxis did not parallel the increase of Col2a1 expression along with chondrogenic induction. The present study demonstrates that the balance between Sox9 and Scleraxis have an important role in the chondrogenic differentiation of ligament-derived cells.
Keywords
Anterior cruciate ligament
Medial collateral ligament
Chondrogenesis
Sox9
Scleraxis
Differentiation
Published Date
2010-01-01
Publication Title
Biochemical and Biophysical Research Communications
Volume
volume391
Issue
issue1
Publisher
Academic Press Inc Elsevier Science
Start Page
1142
End Page
1147
ISSN
0006-291X
NCID
AA00564395
Content Type
Journal Article
language
英語
Copyright Holders
© 2009 Elsevier Inc. All rights reserved.
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Refereed
True
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