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ID 48122
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Author
Ono, Takehiro
Tsuji, Tomoe
Sakai, Miho
Yukizaki, Chizuko
Ino, Hisatoshi
Akagi, Isao
Hiramatsu, Kaori
Sugiura, Yoshihiro
Uto, Hirofumi
Tsubouchi, Hirohito
Abstract
Hepatocyte growth factor (HGF) is useful as a potential therapeutic agent for hepatic and renal fibrosis and cardiovascular diseases through inducing proliferation of epithelial and endothelial cells. HGF inducers may also be useful as therapeutic agents for these diseases. However, there have been no reports on induction of HGF production by plant extracts or juices. An extract of bitter melon (Momordica charantia L.) pulp markedly induced HGF production. There was a time lag of 72 h before induction of HGF production after the extract addition. Its stimulatory effect was accompanied by upregulation of HGF gene expression. Increases in mitogen-activated protein kinases (MAPKs) were observed from 72 h after the extract addition. Inhibitors of MAPKs suppressed the extract-induced HGF production. The extract also stimulated cell proliferation. Both activities for induction of HGF production and cell proliferation were eluted together in a single peak with 14,000 Da on gel filtration. The results indicate that bitter melon pulp extract induced HGF production and cell proliferation of human dermal fibroblasts and suggest that activation of MAPKs is involved in the HGF induction. Our findings suggest potential usefulness of the extract for tissue regeneration and provide an insight into the molecular mechanism underlying the wound-healing property of bitter melon.
Keywords
Hepatocyte growth factor
Bitter melon
Extracellular signal-regulated kinase
Cell proliferation
Dermal fibroblast
Published Date
2009-04
Publication Title
Cytokine
Volume
volume46
Issue
issue1
Publisher
Academic Press Ltd. Elsevier Science Ltd.
Start Page
119
End Page
126
ISSN
1043-4666
NCID
AA10794201
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2009 Elsevier Ltd. All rights reserved.
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Refereed
True
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