start-ver=1.4
cd-journal=joma
no-vol=19
cd-vols=
no-issue=
article-no=
start-page=100665
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190930
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Localization of the ultraviolet-sensor Opn5m and its effect on myopia-related gene expression in the late-embryonic chick eye
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Recent studies show that exposure to ultraviolet (UV) light suppresses ocular elongation, which causes myopia development. However, the specific mechanisms of this process have not been elucidated. A UV-sensor, Opsin 5 (Opn5) mRNA was shown to be present in extraretinal tissues. To test the possibility that UV-signals mediated by Opn5 would have a direct effect on the outer connective tissues of the eye, we first examined the expression patterns of a mammalian type Opn5 (Opn5m) in the late-embryonic chicken eye. Quantitative PCR showed Opn5m mRNA expression in the cornea and sclera. The anti-Opn5m antibody stained a small subset of cells in the corneal stroma and fibrous sclera. We next assessed the effect of UV-A (375 nm) irradiation on the chicken fibroblast cell line DF-1 overexpressing chicken Opn5m. UV-A irradiation for 30 min significantly increased the expression of Early growth response 1 (Egr1), known as an immediate early responsive gene, and of Matrix metalloproteinase 2 (Mmp2) in the presence of retinal chromophore 11-cis-retinal. In contrast, expression of Transforming growth factor beta 2 and Tissue inhibitor of metalloproteinase 2 was not significantly altered. These results indicate that UV-A absorption by Opn5m can upregulate the expression levels of Egr1 and Mmp2 in non-neuronal, fibroblasts. Taken together with the presence of Opn5m in the cornea and sclera, it is suggested that UV-A signaling mediated by Opn5 in the extraretinal ocular tissues could influence directly the outer connective tissues of the chicken late-embryonic eye.
en-copyright=
kn-copyright=

en-aut-name=Kato Mutsuko
en-aut-sei=Kato
en-aut-mei= Mutsuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=Sato Keita
en-aut-sei=Sato
en-aut-mei= Keita
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=Habuta Munenori
en-aut-sei=Habuta
en-aut-mei= Munenori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=Fujita Hirofumi
en-aut-sei=Fujita
en-aut-mei= Hirofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=Bando Tetsuya
en-aut-sei=Bando
en-aut-mei= Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=Morizane Yuki
en-aut-sei=Morizane
en-aut-mei= Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=Shiraga Fumio
en-aut-sei=Shiraga
en-aut-mei= Fumio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=Miyaishi Satoru
en-aut-sei=Miyaishi
en-aut-mei= Satoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=Ohuchi Hideyo
en-aut-sei=Ohuchi
en-aut-mei= Hideyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science
kn-affil=

affil-num=7
en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science
kn-affil=

affil-num=8
en-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=Opsin 5
kn-keyword=Opsin 5
en-keyword=UV-Absorbing pigment
kn-keyword=UV-Absorbing pigment
en-keyword=Fibroblasts
kn-keyword=Fibroblasts
en-keyword=Chicken
kn-keyword=Chicken
en-keyword=Egr1
kn-keyword=Egr1
en-keyword=Mmp2
kn-keyword=Mmp2
END

start-ver=1.4
cd-journal=joma
no-vol=16
cd-vols=
no-issue=
article-no=
start-page=100204
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190930
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effect of intravenous immunoglobulin therapy on anti-NT5C1A antibody-positive inclusion body myositis after successful treatment of hepatitis C: A case report
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Inclusion body myositis (IBM) is the commonest idiopathic inflammatory myopathy of older persons. Pathophysiological mechanism of IBM remains unknown; however, an association of IBM with chronic hepatitis C virus (HCV) infection and serum autoantibodies against skeletal muscle protein 5′-nucleotidase 1A (NT5C1A) has recently been reported. No effective treatment for IBM has yet been developed. We here present a 70-year-old man who was anti-NT5C1A antibody-positive in association with IBM and chronic hepatitis C. The initial treatment of ombitasvir/paritaprevir/ritonavir for his chronic hepatitis C was successful; however, his symptoms of IBM did not improve. On the contrary, his quadriplegic paralysis became more severe and he developed dysphagia. Next, steroid pulse therapy was initiated for IBM and, although his hyper-creatine phosphokinase-emia improved, his symptoms did not; indeed, they worsened. Subsequent intravenous immunoglobulin therapy (IVIg) resulted in obvious improvement in his dysphagia. Thereafter IVIg therapy was repeated at approximately 2-monthly intervals. His dysphagia remained improved for more than 1 year; however, his quadriplegia continued to progress slowly. Although IBM can reportedly be associated with hepatitis C, we inferred that there was no direct relationship between these conditions in our patient because his IBM did not improve after treatment of his hepatitis C. Although his IBM-associated quadriplegia did not improve, IVIg therapy did result in improvement in his dysphagia.
en-copyright=
kn-copyright=

en-aut-name=Takamiya Motonori
en-aut-sei=Takamiya
en-aut-mei= Motonori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=Takahashi Yoshiaki
en-aut-sei=Takahashi
en-aut-mei= Yoshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=Morimoto Mizuki
en-aut-sei=Morimoto
en-aut-mei= Mizuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=Morimoto Nobutoshi
en-aut-sei=Morimoto
en-aut-mei= Nobutoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=Yamashita Satoshi
en-aut-sei=Yamashita
en-aut-mei= Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=Abe Koji
en-aut-sei=Abe
en-aut-mei= Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Department of Neurology, Kagawa Prefectural Central Hospital
kn-affil=

affil-num=2
en-affil=Department of Neurology, Kagawa Prefectural Central Hospital
kn-affil=

affil-num=3
en-affil=Department of Neurology, Kagawa Prefectural Central Hospital
kn-affil=

affil-num=4
en-affil=Department of Neurology, Kagawa Prefectural Central Hospital
kn-affil=

affil-num=5
en-affil=Department of Neurology, Graduate School of Medical Sciences, Kumamoto University
kn-affil=

affil-num=6
en-affil=Department of Neurology, Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University
kn-affil=
en-keyword=Anti-skeletal muscle protein 5′-nucleotidase 1A antibody
kn-keyword=Anti-skeletal muscle protein 5′-nucleotidase 1A antibody
en-keyword= Chronic hepatitis C
kn-keyword= Chronic hepatitis C
en-keyword=Dysphagia
kn-keyword=Dysphagia
en-keyword=Inclusion body myositis
kn-keyword=Inclusion body myositis
en-keyword=Intravenous immunoglobulin therapy
kn-keyword=Intravenous immunoglobulin therapy
END

start-ver=1.4
cd-journal=joma
no-vol=19
cd-vols=
no-issue=2
article-no=
start-page=173
end-page=180
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=201302
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Culture-independent real-time PCR reveals extensive polymicrobial infections in hospitalized diarrhoea cases in Kolkata, India
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Culture-independent identification of diarrhoeal aetiological agents was performed using DNA harvested from diarrhoeal stool specimens with SYBR-Green-based real-time PCR targeting Vibrio cholerae, Vibrio parahaemolyticus, Campylobacter spp., Shigella spp. and three different pathotypes of diarrhoeagenic Escherichia coli. Conventional culture-dependent methods detected bacterial enteropathogens in 68 of 122 diarrhoeal stool specimens. Of 68 specimens, 59 (86.8%) had a single pathogen and the remaining nine (13.2%) had polymicrobial infections with multiple pathogens. Re-analysis of the 68 specimens by culture-independent real-time PCR methods showed that 25 (36.8%) specimens contained single pathogen and 43 (63.2%) specimens contained mixed infections with multiple pathogens. The prevalence of such high levels of polymicrobial infections would not have been detected without using real-time PCR. Culture-dependent analysis assigned 54 of the 122 selected archived specimens as 'no known aetiology'. However, re-analysis of these samples by real-time PCR showed the presence of single or multiple pathogens among 34 (63%) of these specimens. Estimation of relative pathogen load by real-time PCR in the stool specimens indicated that the inability of conventional culture-dependent methods to detect the pathogens was related to lower colony-forming units of the pathogen, as reflected by lower C(t) values. Detection of high levels of polymicrobial infection by real-time PCR indicates that in the settings like Kolkata and its surroundings, where cholera and other enteric diseases are endemic, the concept of one pathogen one disease might need to be re-evaluated.
en-copyright=
kn-copyright=

en-aut-name=SinhaA.
en-aut-sei=Sinha
en-aut-mei=A.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SenGuptaS.
en-aut-sei=SenGupta
en-aut-mei=S.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=GuinS.
en-aut-sei=Guin
en-aut-mei=S.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=DuttaS.
en-aut-sei=Dutta
en-aut-mei=S.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=GhoshS.
en-aut-sei=Ghosh
en-aut-mei=S.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MukherjeeP.
en-aut-sei=Mukherjee
en-aut-mei=P.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=MukhopadhyayA. K.
en-aut-sei=Mukhopadhyay
en-aut-mei=A. K.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=RamamurthyT.
en-aut-sei=Ramamurthy
en-aut-mei=T.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=TakedaY.
en-aut-sei=Takeda
en-aut-mei=Y.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=KurakawaT.
en-aut-sei=Kurakawa
en-aut-mei=T.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=NomotoK.
en-aut-sei=Nomoto
en-aut-mei=K.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=NairG. B.
en-aut-sei=Nair
en-aut-mei=G. B.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=NandyR. K.
en-aut-sei=Nandy
en-aut-mei=R. K.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

affil-num=1
en-affil=National Institute of Cholera and Enteric Diseases (NICED)
kn-affil=

affil-num=2
en-affil=National Institute of Cholera and Enteric Diseases (NICED)
kn-affil=

affil-num=3
en-affil=National Institute of Cholera and Enteric Diseases (NICED)
kn-affil=

affil-num=4
en-affil=National Institute of Cholera and Enteric Diseases (NICED)
kn-affil=

affil-num=5
en-affil=National Institute of Cholera and Enteric Diseases (NICED)
kn-affil=

affil-num=6
en-affil=National Institute of Cholera and Enteric Diseases (NICED)
kn-affil=

affil-num=7
en-affil=National Institute of Cholera and Enteric Diseases (NICED)
kn-affil=

affil-num=8
en-affil=National Institute of Cholera and Enteric Diseases (NICED)
kn-affil=

affil-num=9
en-affil=Collaborative Research Centre of Okayama University for Infectious Diseases in India, NICED
kn-affil=

affil-num=10
en-affil=Yakult Central Institute for Microbiological Research
kn-affil=

affil-num=11
en-affil=Yakult Central Institute for Microbiological Research
kn-affil=

affil-num=12
en-affil=National Institute of Cholera and Enteric Diseases (NICED)
kn-affil=

affil-num=13
en-affil=National Institute of Cholera and Enteric Diseases (NICED)
kn-affil=
en-keyword=Real-time PCR
kn-keyword=Real-time PCR
en-keyword=Diarrhoea
kn-keyword=Diarrhoea
en-keyword=Polymicrobial infection
kn-keyword=Polymicrobial infection
END

start-ver=1.4
cd-journal=joma
no-vol=235
cd-vols=
no-issue=
article-no=
start-page=76
end-page=88
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=20180815
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A simple role of coral-algal symbiosis in coral calcification based on multiple geochemical tracers
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Light-enhanced calcification of reef-building corals, which eventually create vast coral reefs, is well known and based on coral-algal symbiosis. Several controversial hypotheses have been proposed as possible mechanisms for connecting symbiont photosynthesis and coral calcification, including pH rise in the internal pool, role of organic matrix secretion, and enzyme activities. Here, based on the skeletal chemical and isotopic compositions of symbiotic and asymbiotic primary polyps of Acropora digitifera corals, we show a simple pH increase in the calcification medium as the predominant contribution of symbionts to calcification of host corals. We used the symbiotic and asymbiotic primary polyps reared for 10 days at four temperatures (27, 29, 31, and 33 °C), five salinities (34, 32, 30, 28, and 26), and four pCO2 levels (<300, 400, 800, and 1000 µatm). As a result of analyzing multiple geochemical tracers (U/Ca, Mg/Ca, Sr/Ca, δ18O, δ13C, and δ44Ca), a clear and systematic decrease in skeletal U/Ca ratio (used as a proxy for calcification fluid pH) was observed, indicating a higher pH of the fluid in symbiotic compared to asymbiotic polyps. In contrast, Mg/Ca ratios (used as a tentative proxy for organic matrix secretion) and δ44Ca (used as an indicator of Ca2+ pathway to the fluid) did not differ between symbiotic and asymbiotic polyps. This suggests that organic matrix secretion related to coral calcification is controlled mainly by the coral host itself, and a transmembrane transport of Ca2+ does not vary according to symbiosis relationship. Skeletal δ18O values of both symbiotic and asymbiotic polyps showed offsets between them with identical temperature dependence. Based on a newly proposed model, behavior of δ18O in the present study seems to reflect the rate of CO2 hydration in the calcifying fluid. Since CO2 hydration is promoted by enzyme carbonic anhydrase, the offset of δ18O values between symbiotic and asymbiotic polyps is attributed to the differences of enzyme activity, although the enzyme is functional even in the asymbiotic polyp. Symbiotic δ13C values in the temperature and salinity experiments were higher compared to those in the asymbiotic polyps due to photosynthesis, although photosynthetic δ13C signals in the pCO2 experiment were masked by the dominant δ13C gradient in dissolved inorganic carbon in seawater caused by 13C-depletd CO2 gas addition in the higher pCO2 treatments. Sr/Ca ratios showed a negligible relationship according to variation of temperature, salinity, and pCO2, although it might be attributed to relatively large deviations of replicates of Sr/Ca ratios in the present study. Overall, only the U/Ca ratio showed a significant difference between symbiotic and asymbiotic polyps throughout all experiments, indicating that the critical effect on coral calcification caused by symbiotic algae is the increase of pH of the calcifying fluid by photosynthesis.
en-copyright=
kn-copyright=

en-aut-name=InoueMayuri
en-aut-sei=Inoue
en-aut-mei=Mayuri
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NakamuraTakashi
en-aut-sei=Nakamura
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TanakaYasuaki
en-aut-sei=Tanaka
en-aut-mei=Yasuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SuzukiAtsushi
en-aut-sei=Suzuki
en-aut-mei=Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YokoyamaYusuke
en-aut-sei=Yokoyama
en-aut-mei=Yusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KawahataHodaka
en-aut-sei=Kawahata
en-aut-mei=Hodaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=SakaiKazuhiko
en-aut-sei=Sakai
en-aut-mei=Kazuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=GussoneNikolaus
en-aut-sei=Gussone
en-aut-mei=Nikolaus
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Division of Earth Science, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=2
en-affil=Faculty of Science, University of the Ryukyus
kn-affil=

affil-num=3
en-affil=Environmental and Life Sciences, Faculty of Science, Universiti Brunei Darussalam
kn-affil=

affil-num=4
en-affil=Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology
kn-affil=

affil-num=5
en-affil=Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus
kn-affil=

affil-num=6
en-affil=Atmosphere and Ocean Research Institute, The University of Tokyo
kn-affil=

affil-num=7
en-affil=Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus
kn-affil=

affil-num=8
en-affil=Institut für Mineralogie, Universität Münster
kn-affil=
en-keyword=Coral symbiosis
kn-keyword=Coral symbiosis
en-keyword=calcification
kn-keyword=calcification
en-keyword=pH
kn-keyword=pH
en-keyword=geochemical tracers
kn-keyword=geochemical tracers
END

start-ver=1.4
cd-journal=joma
no-vol=57
cd-vols=
no-issue=
article-no=
start-page=404
end-page=413
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=201705
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Biocompatible nanostructured solid adhesives for biological soft tissues
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Over the past few years, the development of novel adhesives for biological soft tissue adhesion has gained significant interest. Such adhesives should be non-toxic and biocompatible. In this study, we synthesized a novel solid adhesive using nanostructured hydroxyapatite (HAp) and evaluated its physical adhesion properties through in vitro testing with synthetic hydrogels and mouse soft tissues. The results revealed that HAp-nanoparticle dispersions and HAp-nanoparticle-assembled nanoporous plates showed efficient adhesion to hydrogels. Interestingly, the HAp plates showed different adhesive properties depending upon the shape of their nanoparticles. The HAp plate made up of 17 nm-sized nanoparticles showed an adhesive strength 2.2 times higher than that of the conventional fibrin glue for mouse skin tissues.
en-copyright=
kn-copyright=

en-aut-name=OkadaMasahiro
en-aut-sei=Okada
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NakaiAkira
en-aut-sei=Nakai
en-aut-mei=Akira
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=Hara EmilioSatoshi
en-aut-sei=Hara Emilio
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TaguchiTetsushi
en-aut-sei=Taguchi
en-aut-mei=Tetsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NakanoTakayoshi
en-aut-sei=Nakano
en-aut-mei=Takayoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MatsumotoTakuya
en-aut-sei=Matsumoto
en-aut-mei=Takuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Biomaterials, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Polymeric Biomaterials Group, RCFM, National Institute for Materials Science
kn-affil=

affil-num=5
en-affil=Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University
kn-affil=

affil-num=6
en-affil=Department of Biomaterials, Okayama University
kn-affil=
en-keyword=Hydroxyapatite
kn-keyword=Hydroxyapatite
en-keyword=Nanoparticle
kn-keyword=Nanoparticle
en-keyword=Solid adhesive
kn-keyword=Solid adhesive
en-keyword=Wet adhesion
kn-keyword=Wet adhesion
END

start-ver=1.4
cd-journal=joma
no-vol=185
cd-vols=
no-issue=
article-no=
start-page=57
end-page=62
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140920
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A rapid and enhanced DNA detection method for crop cultivar discrimination
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In many crops species, the development of a rapid and precise cultivar discrimination system has been required for plant breeding and patent protection of plant cultivars and agricultural products. Here, we successfully evaluated strawberry cultivars via a novel method, namely, the single tag hybridization (STH) chromatographic printed array strip (PAS) using the PCR products of eight genomic regions. In a previous study, we showed that genotyping of eight genomic regions derived from FaRE1 retrotransposon insertion site enabled to discriminate 32 strawberry cultivars precisely, however, this method required agarose/acrylamide gel electrophoresis, thus has the difficulty for practical application. In contrast, novel DNA detection method in this study has some great advantages over standard DNA detection methods, including agarose/acrylamide gel electrophoresis, because it produces signals for DNA detection with dramatically higher sensitivity in a shorter time without any preparation or staining of a gel. Moreover, this method enables the visualization of multiplex signals simultaneously in a single reaction using several independent amplification products. We expect that this novel method will become a rapid and convenient cultivar screening assay for practical purposes, and will be widely applied to various situations, including laboratory research, and on-site inspection of plant cultivars and agricultural products.
en-copyright=
kn-copyright=

en-aut-name=MondenYuki
en-aut-sei=Monden
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TakasakiKazuto
en-aut-sei=Takasaki
en-aut-mei=Kazuto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=FutoSatoshi
en-aut-sei=Futo
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NiwaKousuke
en-aut-sei=Niwa
en-aut-mei=Kousuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KawaseMitsuo
en-aut-sei=Kawase
en-aut-mei=Mitsuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=AkitakeHiroto
en-aut-sei=Akitake
en-aut-mei=Hiroto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TaharaMakoto
en-aut-sei=Tahara
en-aut-mei=Makoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=Graduate School of Environmental and Life Science, Okayama University

affil-num=2
en-affil=
kn-affil=FASMAC Co., Ltd.

affil-num=3
en-affil=
kn-affil=FASMAC Co., Ltd.

affil-num=4
en-affil=
kn-affil=Graduate School of Biomedical Engineering, Tohoku University

affil-num=5
en-affil=
kn-affil=Graduate School of Biomedical Engineering, Tohoku University

affil-num=6
en-affil=
kn-affil=Graduate School of Environmental and Life Science, Okayama University

affil-num=7
en-affil=
kn-affil=Graduate School of Environmental and Life Science, Okayama University
en-keyword=Cultivar discrimination
kn-keyword=Cultivar discrimination
en-keyword=Multiplex PCR
kn-keyword=Multiplex PCR
en-keyword=Strawberry
kn-keyword=Strawberry
en-keyword=Practical application
kn-keyword=Practical application
en-keyword=Retrotransposon
kn-keyword=Retrotransposon
END

start-ver=1.4
cd-journal=joma
no-vol=579
cd-vols=
no-issue=19
article-no=
start-page=4069
end-page=4075
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2005
dt-pub=20050801
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=ZD1839 (Gefitinib, 'Iressa'), an epidermal growth factor receptor-tyrosine kinase inhibitor, enhances the anti-cancer effects of TRAIL in human esophageal squamous cell carcinoma
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The EGF (epidermal growth factor) receptor-tyrosine kinase inhibitor ZD1839 (Gefitinib, 'Iressa') blocks the cell signaling pathways involved in cell proliferation, survival, and angiogenesis in various cancer cells. TNF-related death apoptosis inducing ligand (TRAIL) acts as an anticancer agent. We investigated the antitumor effects of ZD1839 alone or in combination with TRAIL against human esophageal squamous cell cancer (ESCC) lines. Although all ESCC cells expressed EGF receptor at a protein level, the effect of ZD1839 on cell growth did not correlate with the level of EGFR expression and phosphorylation of EGF receptor protein in ESCC lines. ZD1839 caused a dose-dependent growth arrest at G0–G1 phase associated with increased p27 expression. As TE8 cells are resistant to TRAIL, we tested whether ZD1839 combined with TRAIL induced apoptosis of TE8 cells via the inhibition of EGF receptor signaling by ZD1839. ZD1839 inhibited the phosphorylation of Akt, and enhanced TRAIL-induced apoptosis via activation of caspase-3 and caspase-9, and inactivation of Bcl-xL. Our results indicated that ZD1839 has anti-cancer properties against human esophageal cancer cells. ZD1839 also augmented the anti-cancer activity of TRAIL, even in TRAIL-resistant tumors. These results suggest that treatment with ZD1839 and TRAIL may have potential in the treatment of ESCC patients.
en-copyright=
kn-copyright=

en-aut-name=TeraishiFuminori
en-aut-sei=Teraishi
en-aut-mei=Fuminori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KagawaShunsuke
en-aut-sei=Kagawa
en-aut-mei=Shunsuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=WatanabeTakanori
en-aut-sei=Watanabe
en-aut-mei=Takanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TangoYasuhisa
en-aut-sei=Tango
en-aut-mei=Yasuhisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KawashimaTakeshi
en-aut-sei=Kawashima
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=UmeokaTatsuo
en-aut-sei=Umeoka
en-aut-mei=Tatsuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=NisizakiMasahiko
en-aut-sei=Nisizaki
en-aut-mei=Masahiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=TanakaNoriaki
en-aut-sei=Tanaka
en-aut-mei=Noriaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=FujiwaraToshiyoshi
en-aut-sei=Fujiwara
en-aut-mei=Toshiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=
kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry

affil-num=2
en-affil=
kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry

affil-num=3
en-affil=
kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry

affil-num=4
en-affil=
kn-affil=Department of Surgery, Shiga University of Medical Science

affil-num=5
en-affil=
kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry

affil-num=6
en-affil=
kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry

affil-num=7
en-affil=
kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry

affil-num=8
en-affil=
kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry

affil-num=9
en-affil=
kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry
en-keyword=epidermal growth factor receptor
kn-keyword=epidermal growth factor receptor
en-keyword=ZD1839
kn-keyword=ZD1839
en-keyword=akt
kn-keyword=akt
en-keyword=esophageal squamous cell cancer
kn-keyword=esophageal squamous cell cancer
en-keyword=tumor necrosis factor-related apoptosis inducing ligand
kn-keyword=tumor necrosis factor-related apoptosis inducing ligand
END

start-ver=1.4
cd-journal=joma
no-vol=43
cd-vols=
no-issue=2
article-no=
start-page=125
end-page=131
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2005
dt-pub=20050201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Neutrophil and lymphocyte responses to oral Streptococcus in Adamantiades-Behcet's disease
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Immune reactions against microorganisms play an important pathogenic role in Adamantiades-Behçet’s disease (ABD). We had previously obtained Streptococcus sanguinis (strain BD113-20) isolated from the oral cavity of patients with ABD. To investigate the pathogenesis of this isolate, we examined neutrophil 5 reactions and level of cytokine production by lymphocytes after stimulation with the strain. The reactions
of neutrophils were examined by chemiluminescence assay using whole blood. The
amounts of interferon gamma (IFN-g) and interleukin (IL)-4, IL-8, IL-10, and IL-12
produced by peripheral blood mononuclear cells (PBMCs) were measured by ELISA. 10 Strain BD113-20 activated neutrophils from patients with ABD and healthy volunteers, and, in addition it increased IFN-g production by lymphocytes. Lymphocyte from the patients with ABD showed a dominant T helper 1 (Th-1) immune response. Results indicated that both bacterial stimulation and host hypersensitivity might be involved in the symptoms and pathogenesis of ABD.
en-copyright=
kn-copyright=

en-aut-name=KurauchiTomomi
en-aut-sei=Kurauchi
en-aut-mei=Tomomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YokotaKenji
en-aut-sei=Yokota
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=FujinamiYoshihito
en-aut-sei=Fujinami
en-aut-mei=Yoshihito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=IsogaiEmiko
en-aut-sei=Isogai
en-aut-mei=Emiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=IsogaiHiroshi
en-aut-sei=Isogai
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=OhtsukiHiroshi
en-aut-sei=Ohtsuki
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=OgumaKeiji
en-aut-sei=Oguma
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry

affil-num=2
en-affil=
kn-affil=Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry

affil-num=3
en-affil=
kn-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry

affil-num=4
en-affil=
kn-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry

affil-num=5
en-affil=
kn-affil=Department of Preventive Dentistry, Health Sciences University of Hokkaido

affil-num=6
en-affil=
kn-affil=Division of Animal Experimentation, Sapporo Medical University

affil-num=7
en-affil=
kn-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry

affil-num=8
en-affil=
kn-affil=Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry
en-keyword=Adamantiades-Behcet's disease
kn-keyword=Adamantiades-Behcet's disease
en-keyword=Streptococcus sanguinis
kn-keyword=Streptococcus sanguinis
en-keyword=neutrophil
kn-keyword=neutrophil
en-keyword=chemiluminescence
kn-keyword=chemiluminescence
en-keyword=IL-8
kn-keyword=IL-8
en-keyword=T helper-1
kn-keyword=T helper-1
en-keyword=IFN-gamma
kn-keyword=IFN-gamma
en-keyword=IL-12
kn-keyword=IL-12
END

start-ver=1.4
cd-journal=joma
no-vol=1774
cd-vols=
no-issue=4
article-no=
start-page=456
end-page=465
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2007
dt-pub=200704
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Tissue-specific interactions of TNI isoforms with other TN subunits and tropomyosins in C. elegans: The role of the C- and N-terminal extensions
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The aim of this study is to investigate the function of the C-terminal extension of three troponin I isoforms, that are unique to the body wall muscles of Caenorhabditis elegans and to understand the molecular interactions within the TN complex between troponin I with troponin C/T, and tropomyosin. We constructed several expression vectors to generate recombinant proteins of three body wall and one pharyngeal troponin I isoforms in Escherichia coli. Protein overlay assays and Western blot analyses were performed using antibodies. We demonstrated that pharyngeal TNI-4 interacted with only the pharyngeal isoforms of troponin C/T and tropomyosin. In contrast, the body wall TNI-2 bound both the body wall and pharyngeal isoforms of these components. Similar to other invertebrates, the N-terminus of troponin I contributes to interactions with troponin C. Full-length troponin I was essential for interactions with tropomyosin isoforms. Deletion of the C-terminal extension had no direct effect on the binding of the body wall troponin I to other muscle thin filament troponin C/T and tropomyosin isoforms.
en-copyright=
kn-copyright=

en-aut-name=AminMd. Ziaul
en-aut-sei=Amin
en-aut-mei=Md. Ziaul
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=BandoTetsuya
en-aut-sei=Bando
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=RuksanaRazia
en-aut-sei=Ruksana
en-aut-mei=Razia
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=Anokye-DansoFrederick
en-aut-sei=Anokye-Danso
en-aut-mei=Frederick
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TakashimaYasuo
en-aut-sei=Takashima
en-aut-mei=Yasuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=SakubeYasuji
en-aut-sei=Sakube
en-aut-mei=Yasuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KagawaHiroaki
en-aut-sei=Kagawa
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

affil-num=2
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

affil-num=3
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

affil-num=4
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

affil-num=5
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

affil-num=6
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

affil-num=7
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
en-keyword=Troponin I
kn-keyword=Troponin I
en-keyword=Caenorhabditis elegans
kn-keyword=Caenorhabditis elegans
en-keyword=Troponin T
kn-keyword=Troponin T
en-keyword=Troponin C
kn-keyword=Troponin C
en-keyword=Tropomyosin
kn-keyword=Tropomyosin
en-keyword=C-terminal extension
kn-keyword=C-terminal extension
END

start-ver=1.4
cd-journal=joma
no-vol=9
cd-vols=
no-issue=9
article-no=
start-page=1913
end-page=1915
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=20080515
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=CO oxidation on perovskite-type LaCoO3 synthesized using ethylene glycol and citric acid
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to synthesize perovskite-type LaCoO3 with good surface crystallinity, the gel prepared by adding both ethylene glycol (EG) and citric acid (CA) to the aqueous solution of La(NO3)3 center dot 6H(2)O and Co(NO3)(2) center dot 6H(2)O was fired at 600 degrees C in air for 3 h. The transmission electron microscopy (TEM) observation indicated that the particles of LaCoO3 tended to have a uniform shape at EG/CA = 4. Although, the specific surface area of LaCoO3 synthesized using both EG and CA was slightly smaller than that of LaCoO3 synthesized using only CA, the catalytic activity of CO oxidation became higher by adding EG.
en-copyright=
kn-copyright=

en-aut-name=TaguchiHideki
en-aut-sei=Taguchi
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YamasakiSatoshi
en-aut-sei=Yamasaki
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ItadaniAtsushi
en-aut-sei=Itadani
en-aut-mei=Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YosinagaMasashi
en-aut-sei=Yosinaga
en-aut-mei=Masashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=HirotaKen
en-aut-sei=Hirota
en-aut-mei=Ken
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=The Graduate School of Natural Science and Technology (Science), Okayama University

affil-num=2
en-affil=
kn-affil=The Graduate School of Natural Science and Technology (Science), Okayama University

affil-num=3
en-affil=
kn-affil=The Graduate School of Natural Science and Technology (Science), Okayama University

affil-num=4
en-affil=
kn-affil=Kyoto Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence, JST

affil-num=5
en-affil=
kn-affil=Faculty of Engineering, Doshisha University
en-keyword=LaCoO3
kn-keyword=LaCoO3
en-keyword=perovskite
kn-keyword=perovskite
en-keyword=sol-gel preparation
kn-keyword=sol-gel preparation
en-keyword=CO oxidation
kn-keyword=CO oxidation
END