start-ver=1.4 cd-journal=joma no-vol=19 cd-vols= no-issue= article-no= start-page=100665 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Localization of the ultraviolet-sensor Opn5m and its effect on myopia-related gene expression in the late-embryonic chick eye en-subtitle= kn-subtitle= en-abstract= kn-abstract= Recent studies show that exposure to ultraviolet (UV) light suppresses ocular elongation, which causes myopia development. However, the specific mechanisms of this process have not been elucidated. A UV-sensor, Opsin 5 (Opn5) mRNA was shown to be present in extraretinal tissues. To test the possibility that UV-signals mediated by Opn5 would have a direct effect on the outer connective tissues of the eye, we first examined the expression patterns of a mammalian type Opn5 (Opn5m) in the late-embryonic chicken eye. Quantitative PCR showed Opn5m mRNA expression in the cornea and sclera. The anti-Opn5m antibody stained a small subset of cells in the corneal stroma and fibrous sclera. We next assessed the effect of UV-A (375 nm) irradiation on the chicken fibroblast cell line DF-1 overexpressing chicken Opn5m. UV-A irradiation for 30 min significantly increased the expression of Early growth response 1 (Egr1), known as an immediate early responsive gene, and of Matrix metalloproteinase 2 (Mmp2) in the presence of retinal chromophore 11-cis-retinal. In contrast, expression of Transforming growth factor beta 2 and Tissue inhibitor of metalloproteinase 2 was not significantly altered. These results indicate that UV-A absorption by Opn5m can upregulate the expression levels of Egr1 and Mmp2 in non-neuronal, fibroblasts. Taken together with the presence of Opn5m in the cornea and sclera, it is suggested that UV-A signaling mediated by Opn5 in the extraretinal ocular tissues could influence directly the outer connective tissues of the chicken late-embryonic eye. en-copyright= kn-copyright= en-aut-name=Kato Mutsuko en-aut-sei=Kato en-aut-mei= Mutsuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=Sato Keita en-aut-sei=Sato en-aut-mei= Keita kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=Habuta Munenori en-aut-sei=Habuta en-aut-mei= Munenori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=Fujita Hirofumi en-aut-sei=Fujita en-aut-mei= Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=Bando Tetsuya en-aut-sei=Bando en-aut-mei= Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=Morizane Yuki en-aut-sei=Morizane en-aut-mei= Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=Shiraga Fumio en-aut-sei=Shiraga en-aut-mei= Fumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=Miyaishi Satoru en-aut-sei=Miyaishi en-aut-mei= Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=Ohuchi Hideyo en-aut-sei=Ohuchi en-aut-mei= Hideyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science kn-affil= affil-num=7 en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science kn-affil= affil-num=8 en-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=Opsin 5 kn-keyword=Opsin 5 en-keyword=UV-Absorbing pigment kn-keyword=UV-Absorbing pigment en-keyword=Fibroblasts kn-keyword=Fibroblasts en-keyword=Chicken kn-keyword=Chicken en-keyword=Egr1 kn-keyword=Egr1 en-keyword=Mmp2 kn-keyword=Mmp2 END start-ver=1.4 cd-journal=joma no-vol=16 cd-vols= no-issue= article-no= start-page=100204 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of intravenous immunoglobulin therapy on anti-NT5C1A antibody-positive inclusion body myositis after successful treatment of hepatitis C: A case report en-subtitle= kn-subtitle= en-abstract= kn-abstract= Inclusion body myositis (IBM) is the commonest idiopathic inflammatory myopathy of older persons. Pathophysiological mechanism of IBM remains unknown; however, an association of IBM with chronic hepatitis C virus (HCV) infection and serum autoantibodies against skeletal muscle protein 5′-nucleotidase 1A (NT5C1A) has recently been reported. No effective treatment for IBM has yet been developed. We here present a 70-year-old man who was anti-NT5C1A antibody-positive in association with IBM and chronic hepatitis C. The initial treatment of ombitasvir/paritaprevir/ritonavir for his chronic hepatitis C was successful; however, his symptoms of IBM did not improve. On the contrary, his quadriplegic paralysis became more severe and he developed dysphagia. Next, steroid pulse therapy was initiated for IBM and, although his hyper-creatine phosphokinase-emia improved, his symptoms did not; indeed, they worsened. Subsequent intravenous immunoglobulin therapy (IVIg) resulted in obvious improvement in his dysphagia. Thereafter IVIg therapy was repeated at approximately 2-monthly intervals. His dysphagia remained improved for more than 1 year; however, his quadriplegia continued to progress slowly. Although IBM can reportedly be associated with hepatitis C, we inferred that there was no direct relationship between these conditions in our patient because his IBM did not improve after treatment of his hepatitis C. Although his IBM-associated quadriplegia did not improve, IVIg therapy did result in improvement in his dysphagia. en-copyright= kn-copyright= en-aut-name=Takamiya Motonori en-aut-sei=Takamiya en-aut-mei= Motonori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=Takahashi Yoshiaki en-aut-sei=Takahashi en-aut-mei= Yoshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=Morimoto Mizuki en-aut-sei=Morimoto en-aut-mei= Mizuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=Morimoto Nobutoshi en-aut-sei=Morimoto en-aut-mei= Nobutoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=Yamashita Satoshi en-aut-sei=Yamashita en-aut-mei= Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=Abe Koji en-aut-sei=Abe en-aut-mei= Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Department of Neurology, Kagawa Prefectural Central Hospital kn-affil= affil-num=2 en-affil=Department of Neurology, Kagawa Prefectural Central Hospital kn-affil= affil-num=3 en-affil=Department of Neurology, Kagawa Prefectural Central Hospital kn-affil= affil-num=4 en-affil=Department of Neurology, Kagawa Prefectural Central Hospital kn-affil= affil-num=5 en-affil=Department of Neurology, Graduate School of Medical Sciences, Kumamoto University kn-affil= affil-num=6 en-affil=Department of Neurology, Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University kn-affil= en-keyword=Anti-skeletal muscle protein 5′-nucleotidase 1A antibody kn-keyword=Anti-skeletal muscle protein 5′-nucleotidase 1A antibody en-keyword= Chronic hepatitis C kn-keyword= Chronic hepatitis C en-keyword=Dysphagia kn-keyword=Dysphagia en-keyword=Inclusion body myositis kn-keyword=Inclusion body myositis en-keyword=Intravenous immunoglobulin therapy kn-keyword=Intravenous immunoglobulin therapy END start-ver=1.4 cd-journal=joma no-vol=19 cd-vols= no-issue=2 article-no= start-page=173 end-page=180 dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=201302 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Culture-independent real-time PCR reveals extensive polymicrobial infections in hospitalized diarrhoea cases in Kolkata, India en-subtitle= kn-subtitle= en-abstract= kn-abstract= Culture-independent identification of diarrhoeal aetiological agents was performed using DNA harvested from diarrhoeal stool specimens with SYBR-Green-based real-time PCR targeting Vibrio cholerae, Vibrio parahaemolyticus, Campylobacter spp., Shigella spp. and three different pathotypes of diarrhoeagenic Escherichia coli. Conventional culture-dependent methods detected bacterial enteropathogens in 68 of 122 diarrhoeal stool specimens. Of 68 specimens, 59 (86.8%) had a single pathogen and the remaining nine (13.2%) had polymicrobial infections with multiple pathogens. Re-analysis of the 68 specimens by culture-independent real-time PCR methods showed that 25 (36.8%) specimens contained single pathogen and 43 (63.2%) specimens contained mixed infections with multiple pathogens. The prevalence of such high levels of polymicrobial infections would not have been detected without using real-time PCR. Culture-dependent analysis assigned 54 of the 122 selected archived specimens as 'no known aetiology'. However, re-analysis of these samples by real-time PCR showed the presence of single or multiple pathogens among 34 (63%) of these specimens. Estimation of relative pathogen load by real-time PCR in the stool specimens indicated that the inability of conventional culture-dependent methods to detect the pathogens was related to lower colony-forming units of the pathogen, as reflected by lower C(t) values. Detection of high levels of polymicrobial infection by real-time PCR indicates that in the settings like Kolkata and its surroundings, where cholera and other enteric diseases are endemic, the concept of one pathogen one disease might need to be re-evaluated. en-copyright= kn-copyright= en-aut-name=SinhaA. en-aut-sei=Sinha en-aut-mei=A. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SenGuptaS. en-aut-sei=SenGupta en-aut-mei=S. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=GuinS. en-aut-sei=Guin en-aut-mei=S. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=DuttaS. en-aut-sei=Dutta en-aut-mei=S. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=GhoshS. en-aut-sei=Ghosh en-aut-mei=S. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MukherjeeP. en-aut-sei=Mukherjee en-aut-mei=P. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MukhopadhyayA. K. en-aut-sei=Mukhopadhyay en-aut-mei=A. K. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=RamamurthyT. en-aut-sei=Ramamurthy en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TakedaY. en-aut-sei=Takeda en-aut-mei=Y. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=KurakawaT. en-aut-sei=Kurakawa en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=NomotoK. en-aut-sei=Nomoto en-aut-mei=K. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=NairG. B. en-aut-sei=Nair en-aut-mei=G. B. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=NandyR. K. en-aut-sei=Nandy en-aut-mei=R. K. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= affil-num=1 en-affil=National Institute of Cholera and Enteric Diseases (NICED) kn-affil= affil-num=2 en-affil=National Institute of Cholera and Enteric Diseases (NICED) kn-affil= affil-num=3 en-affil=National Institute of Cholera and Enteric Diseases (NICED) kn-affil= affil-num=4 en-affil=National Institute of Cholera and Enteric Diseases (NICED) kn-affil= affil-num=5 en-affil=National Institute of Cholera and Enteric Diseases (NICED) kn-affil= affil-num=6 en-affil=National Institute of Cholera and Enteric Diseases (NICED) kn-affil= affil-num=7 en-affil=National Institute of Cholera and Enteric Diseases (NICED) kn-affil= affil-num=8 en-affil=National Institute of Cholera and Enteric Diseases (NICED) kn-affil= affil-num=9 en-affil=Collaborative Research Centre of Okayama University for Infectious Diseases in India, NICED kn-affil= affil-num=10 en-affil=Yakult Central Institute for Microbiological Research kn-affil= affil-num=11 en-affil=Yakult Central Institute for Microbiological Research kn-affil= affil-num=12 en-affil=National Institute of Cholera and Enteric Diseases (NICED) kn-affil= affil-num=13 en-affil=National Institute of Cholera and Enteric Diseases (NICED) kn-affil= en-keyword=Real-time PCR kn-keyword=Real-time PCR en-keyword=Diarrhoea kn-keyword=Diarrhoea en-keyword=Polymicrobial infection kn-keyword=Polymicrobial infection END start-ver=1.4 cd-journal=joma no-vol=235 cd-vols= no-issue= article-no= start-page=76 end-page=88 dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=20180815 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A simple role of coral-algal symbiosis in coral calcification based on multiple geochemical tracers en-subtitle= kn-subtitle= en-abstract= kn-abstract= Light-enhanced calcification of reef-building corals, which eventually create vast coral reefs, is well known and based on coral-algal symbiosis. Several controversial hypotheses have been proposed as possible mechanisms for connecting symbiont photosynthesis and coral calcification, including pH rise in the internal pool, role of organic matrix secretion, and enzyme activities. Here, based on the skeletal chemical and isotopic compositions of symbiotic and asymbiotic primary polyps of Acropora digitifera corals, we show a simple pH increase in the calcification medium as the predominant contribution of symbionts to calcification of host corals. We used the symbiotic and asymbiotic primary polyps reared for 10 days at four temperatures (27, 29, 31, and 33 °C), five salinities (34, 32, 30, 28, and 26), and four pCO2 levels (<300, 400, 800, and 1000 µatm). As a result of analyzing multiple geochemical tracers (U/Ca, Mg/Ca, Sr/Ca, δ18O, δ13C, and δ44Ca), a clear and systematic decrease in skeletal U/Ca ratio (used as a proxy for calcification fluid pH) was observed, indicating a higher pH of the fluid in symbiotic compared to asymbiotic polyps. In contrast, Mg/Ca ratios (used as a tentative proxy for organic matrix secretion) and δ44Ca (used as an indicator of Ca2+ pathway to the fluid) did not differ between symbiotic and asymbiotic polyps. This suggests that organic matrix secretion related to coral calcification is controlled mainly by the coral host itself, and a transmembrane transport of Ca2+ does not vary according to symbiosis relationship. Skeletal δ18O values of both symbiotic and asymbiotic polyps showed offsets between them with identical temperature dependence. Based on a newly proposed model, behavior of δ18O in the present study seems to reflect the rate of CO2 hydration in the calcifying fluid. Since CO2 hydration is promoted by enzyme carbonic anhydrase, the offset of δ18O values between symbiotic and asymbiotic polyps is attributed to the differences of enzyme activity, although the enzyme is functional even in the asymbiotic polyp. Symbiotic δ13C values in the temperature and salinity experiments were higher compared to those in the asymbiotic polyps due to photosynthesis, although photosynthetic δ13C signals in the pCO2 experiment were masked by the dominant δ13C gradient in dissolved inorganic carbon in seawater caused by 13C-depletd CO2 gas addition in the higher pCO2 treatments. Sr/Ca ratios showed a negligible relationship according to variation of temperature, salinity, and pCO2, although it might be attributed to relatively large deviations of replicates of Sr/Ca ratios in the present study. Overall, only the U/Ca ratio showed a significant difference between symbiotic and asymbiotic polyps throughout all experiments, indicating that the critical effect on coral calcification caused by symbiotic algae is the increase of pH of the calcifying fluid by photosynthesis. en-copyright= kn-copyright= en-aut-name=InoueMayuri en-aut-sei=Inoue en-aut-mei=Mayuri kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakamuraTakashi en-aut-sei=Nakamura en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TanakaYasuaki en-aut-sei=Tanaka en-aut-mei=Yasuaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SuzukiAtsushi en-aut-sei=Suzuki en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YokoyamaYusuke en-aut-sei=Yokoyama en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KawahataHodaka en-aut-sei=Kawahata en-aut-mei=Hodaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SakaiKazuhiko en-aut-sei=Sakai en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=GussoneNikolaus en-aut-sei=Gussone en-aut-mei=Nikolaus kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil=Division of Earth Science, Graduate School of Natural Science and Technology, Okayama University kn-affil= affil-num=2 en-affil=Faculty of Science, University of the Ryukyus kn-affil= affil-num=3 en-affil=Environmental and Life Sciences, Faculty of Science, Universiti Brunei Darussalam kn-affil= affil-num=4 en-affil=Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology kn-affil= affil-num=5 en-affil=Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus kn-affil= affil-num=6 en-affil=Atmosphere and Ocean Research Institute, The University of Tokyo kn-affil= affil-num=7 en-affil=Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus kn-affil= affil-num=8 en-affil=Institut für Mineralogie, Universität Münster kn-affil= en-keyword=Coral symbiosis kn-keyword=Coral symbiosis en-keyword=calcification kn-keyword=calcification en-keyword=pH kn-keyword=pH en-keyword=geochemical tracers kn-keyword=geochemical tracers END start-ver=1.4 cd-journal=joma no-vol=57 cd-vols= no-issue= article-no= start-page=404 end-page=413 dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=201705 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Biocompatible nanostructured solid adhesives for biological soft tissues en-subtitle= kn-subtitle= en-abstract= kn-abstract= Over the past few years, the development of novel adhesives for biological soft tissue adhesion has gained significant interest. Such adhesives should be non-toxic and biocompatible. In this study, we synthesized a novel solid adhesive using nanostructured hydroxyapatite (HAp) and evaluated its physical adhesion properties through in vitro testing with synthetic hydrogels and mouse soft tissues. The results revealed that HAp-nanoparticle dispersions and HAp-nanoparticle-assembled nanoporous plates showed efficient adhesion to hydrogels. Interestingly, the HAp plates showed different adhesive properties depending upon the shape of their nanoparticles. The HAp plate made up of 17 nm-sized nanoparticles showed an adhesive strength 2.2 times higher than that of the conventional fibrin glue for mouse skin tissues. en-copyright= kn-copyright= en-aut-name=OkadaMasahiro en-aut-sei=Okada en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakaiAkira en-aut-sei=Nakai en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=Hara EmilioSatoshi en-aut-sei=Hara Emilio en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TaguchiTetsushi en-aut-sei=Taguchi en-aut-mei=Tetsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakanoTakayoshi en-aut-sei=Nakano en-aut-mei=Takayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MatsumotoTakuya en-aut-sei=Matsumoto en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=2 en-affil=Department of Biomaterials, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=3 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=4 en-affil=Polymeric Biomaterials Group, RCFM, National Institute for Materials Science kn-affil= affil-num=5 en-affil=Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University kn-affil= affil-num=6 en-affil=Department of Biomaterials, Okayama University kn-affil= en-keyword=Hydroxyapatite kn-keyword=Hydroxyapatite en-keyword=Nanoparticle kn-keyword=Nanoparticle en-keyword=Solid adhesive kn-keyword=Solid adhesive en-keyword=Wet adhesion kn-keyword=Wet adhesion END start-ver=1.4 cd-journal=joma no-vol=185 cd-vols= no-issue= article-no= start-page=57 end-page=62 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140920 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A rapid and enhanced DNA detection method for crop cultivar discrimination en-subtitle= kn-subtitle= en-abstract= kn-abstract=In many crops species, the development of a rapid and precise cultivar discrimination system has been required for plant breeding and patent protection of plant cultivars and agricultural products. Here, we successfully evaluated strawberry cultivars via a novel method, namely, the single tag hybridization (STH) chromatographic printed array strip (PAS) using the PCR products of eight genomic regions. In a previous study, we showed that genotyping of eight genomic regions derived from FaRE1 retrotransposon insertion site enabled to discriminate 32 strawberry cultivars precisely, however, this method required agarose/acrylamide gel electrophoresis, thus has the difficulty for practical application. In contrast, novel DNA detection method in this study has some great advantages over standard DNA detection methods, including agarose/acrylamide gel electrophoresis, because it produces signals for DNA detection with dramatically higher sensitivity in a shorter time without any preparation or staining of a gel. Moreover, this method enables the visualization of multiplex signals simultaneously in a single reaction using several independent amplification products. We expect that this novel method will become a rapid and convenient cultivar screening assay for practical purposes, and will be widely applied to various situations, including laboratory research, and on-site inspection of plant cultivars and agricultural products. en-copyright= kn-copyright= en-aut-name=MondenYuki en-aut-sei=Monden en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakasakiKazuto en-aut-sei=Takasaki en-aut-mei=Kazuto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FutoSatoshi en-aut-sei=Futo en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NiwaKousuke en-aut-sei=Niwa en-aut-mei=Kousuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KawaseMitsuo en-aut-sei=Kawase en-aut-mei=Mitsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=AkitakeHiroto en-aut-sei=Akitake en-aut-mei=Hiroto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TaharaMakoto en-aut-sei=Tahara en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Environmental and Life Science, Okayama University affil-num=2 en-affil= kn-affil=FASMAC Co., Ltd. affil-num=3 en-affil= kn-affil=FASMAC Co., Ltd. affil-num=4 en-affil= kn-affil=Graduate School of Biomedical Engineering, Tohoku University affil-num=5 en-affil= kn-affil=Graduate School of Biomedical Engineering, Tohoku University affil-num=6 en-affil= kn-affil=Graduate School of Environmental and Life Science, Okayama University affil-num=7 en-affil= kn-affil=Graduate School of Environmental and Life Science, Okayama University en-keyword=Cultivar discrimination kn-keyword=Cultivar discrimination en-keyword=Multiplex PCR kn-keyword=Multiplex PCR en-keyword=Strawberry kn-keyword=Strawberry en-keyword=Practical application kn-keyword=Practical application en-keyword=Retrotransposon kn-keyword=Retrotransposon END start-ver=1.4 cd-journal=joma no-vol=579 cd-vols= no-issue=19 article-no= start-page=4069 end-page=4075 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050801 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ZD1839 (Gefitinib, 'Iressa'), an epidermal growth factor receptor-tyrosine kinase inhibitor, enhances the anti-cancer effects of TRAIL in human esophageal squamous cell carcinoma en-subtitle= kn-subtitle= en-abstract= kn-abstract=The EGF (epidermal growth factor) receptor-tyrosine kinase inhibitor ZD1839 (Gefitinib, 'Iressa') blocks the cell signaling pathways involved in cell proliferation, survival, and angiogenesis in various cancer cells. TNF-related death apoptosis inducing ligand (TRAIL) acts as an anticancer agent. We investigated the antitumor effects of ZD1839 alone or in combination with TRAIL against human esophageal squamous cell cancer (ESCC) lines. Although all ESCC cells expressed EGF receptor at a protein level, the effect of ZD1839 on cell growth did not correlate with the level of EGFR expression and phosphorylation of EGF receptor protein in ESCC lines. ZD1839 caused a dose-dependent growth arrest at G0–G1 phase associated with increased p27 expression. As TE8 cells are resistant to TRAIL, we tested whether ZD1839 combined with TRAIL induced apoptosis of TE8 cells via the inhibition of EGF receptor signaling by ZD1839. ZD1839 inhibited the phosphorylation of Akt, and enhanced TRAIL-induced apoptosis via activation of caspase-3 and caspase-9, and inactivation of Bcl-xL. Our results indicated that ZD1839 has anti-cancer properties against human esophageal cancer cells. ZD1839 also augmented the anti-cancer activity of TRAIL, even in TRAIL-resistant tumors. These results suggest that treatment with ZD1839 and TRAIL may have potential in the treatment of ESCC patients. en-copyright= kn-copyright= en-aut-name=TeraishiFuminori en-aut-sei=Teraishi en-aut-mei=Fuminori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KagawaShunsuke en-aut-sei=Kagawa en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WatanabeTakanori en-aut-sei=Watanabe en-aut-mei=Takanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TangoYasuhisa en-aut-sei=Tango en-aut-mei=Yasuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KawashimaTakeshi en-aut-sei=Kawashima en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=UmeokaTatsuo en-aut-sei=Umeoka en-aut-mei=Tatsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NisizakiMasahiko en-aut-sei=Nisizaki en-aut-mei=Masahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=FujiwaraToshiyoshi en-aut-sei=Fujiwara en-aut-mei=Toshiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry affil-num=2 en-affil= kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry affil-num=3 en-affil= kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry affil-num=4 en-affil= kn-affil=Department of Surgery, Shiga University of Medical Science affil-num=5 en-affil= kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry affil-num=6 en-affil= kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry affil-num=7 en-affil= kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry affil-num=8 en-affil= kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry affil-num=9 en-affil= kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry en-keyword=epidermal growth factor receptor kn-keyword=epidermal growth factor receptor en-keyword=ZD1839 kn-keyword=ZD1839 en-keyword=akt kn-keyword=akt en-keyword=esophageal squamous cell cancer kn-keyword=esophageal squamous cell cancer en-keyword=tumor necrosis factor-related apoptosis inducing ligand kn-keyword=tumor necrosis factor-related apoptosis inducing ligand END start-ver=1.4 cd-journal=joma no-vol=43 cd-vols= no-issue=2 article-no= start-page=125 end-page=131 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Neutrophil and lymphocyte responses to oral Streptococcus in Adamantiades-Behcet's disease en-subtitle= kn-subtitle= en-abstract= kn-abstract=Immune reactions against microorganisms play an important pathogenic role in Adamantiades-Behçet’s disease (ABD). We had previously obtained Streptococcus sanguinis (strain BD113-20) isolated from the oral cavity of patients with ABD. To investigate the pathogenesis of this isolate, we examined neutrophil 5 reactions and level of cytokine production by lymphocytes after stimulation with the strain. The reactions of neutrophils were examined by chemiluminescence assay using whole blood. The amounts of interferon gamma (IFN-g) and interleukin (IL)-4, IL-8, IL-10, and IL-12 produced by peripheral blood mononuclear cells (PBMCs) were measured by ELISA. 10 Strain BD113-20 activated neutrophils from patients with ABD and healthy volunteers, and, in addition it increased IFN-g production by lymphocytes. Lymphocyte from the patients with ABD showed a dominant T helper 1 (Th-1) immune response. Results indicated that both bacterial stimulation and host hypersensitivity might be involved in the symptoms and pathogenesis of ABD. en-copyright= kn-copyright= en-aut-name=KurauchiTomomi en-aut-sei=Kurauchi en-aut-mei=Tomomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YokotaKenji en-aut-sei=Yokota en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MatsuoToshihiko en-aut-sei=Matsuo en-aut-mei=Toshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FujinamiYoshihito en-aut-sei=Fujinami en-aut-mei=Yoshihito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=IsogaiEmiko en-aut-sei=Isogai en-aut-mei=Emiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IsogaiHiroshi en-aut-sei=Isogai en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OhtsukiHiroshi en-aut-sei=Ohtsuki en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OgumaKeiji en-aut-sei=Oguma en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry affil-num=2 en-affil= kn-affil=Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry affil-num=3 en-affil= kn-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry affil-num=4 en-affil= kn-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry affil-num=5 en-affil= kn-affil=Department of Preventive Dentistry, Health Sciences University of Hokkaido affil-num=6 en-affil= kn-affil=Division of Animal Experimentation, Sapporo Medical University affil-num=7 en-affil= kn-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry affil-num=8 en-affil= kn-affil=Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry en-keyword=Adamantiades-Behcet's disease kn-keyword=Adamantiades-Behcet's disease en-keyword=Streptococcus sanguinis kn-keyword=Streptococcus sanguinis en-keyword=neutrophil kn-keyword=neutrophil en-keyword=chemiluminescence kn-keyword=chemiluminescence en-keyword=IL-8 kn-keyword=IL-8 en-keyword=T helper-1 kn-keyword=T helper-1 en-keyword=IFN-gamma kn-keyword=IFN-gamma en-keyword=IL-12 kn-keyword=IL-12 END start-ver=1.4 cd-journal=joma no-vol=1774 cd-vols= no-issue=4 article-no= start-page=456 end-page=465 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=200704 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Tissue-specific interactions of TNI isoforms with other TN subunits and tropomyosins in C. elegans: The role of the C- and N-terminal extensions en-subtitle= kn-subtitle= en-abstract= kn-abstract=The aim of this study is to investigate the function of the C-terminal extension of three troponin I isoforms, that are unique to the body wall muscles of Caenorhabditis elegans and to understand the molecular interactions within the TN complex between troponin I with troponin C/T, and tropomyosin. We constructed several expression vectors to generate recombinant proteins of three body wall and one pharyngeal troponin I isoforms in Escherichia coli. Protein overlay assays and Western blot analyses were performed using antibodies. We demonstrated that pharyngeal TNI-4 interacted with only the pharyngeal isoforms of troponin C/T and tropomyosin. In contrast, the body wall TNI-2 bound both the body wall and pharyngeal isoforms of these components. Similar to other invertebrates, the N-terminus of troponin I contributes to interactions with troponin C. Full-length troponin I was essential for interactions with tropomyosin isoforms. Deletion of the C-terminal extension had no direct effect on the binding of the body wall troponin I to other muscle thin filament troponin C/T and tropomyosin isoforms. en-copyright= kn-copyright= en-aut-name=AminMd. Ziaul en-aut-sei=Amin en-aut-mei=Md. Ziaul kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=BandoTetsuya en-aut-sei=Bando en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=RuksanaRazia en-aut-sei=Ruksana en-aut-mei=Razia kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=Anokye-DansoFrederick en-aut-sei=Anokye-Danso en-aut-mei=Frederick kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TakashimaYasuo en-aut-sei=Takashima en-aut-mei=Yasuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SakubeYasuji en-aut-sei=Sakube en-aut-mei=Yasuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KagawaHiroaki en-aut-sei=Kagawa en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University affil-num=5 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University affil-num=6 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University affil-num=7 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University en-keyword=Troponin I kn-keyword=Troponin I en-keyword=Caenorhabditis elegans kn-keyword=Caenorhabditis elegans en-keyword=Troponin T kn-keyword=Troponin T en-keyword=Troponin C kn-keyword=Troponin C en-keyword=Tropomyosin kn-keyword=Tropomyosin en-keyword=C-terminal extension kn-keyword=C-terminal extension END start-ver=1.4 cd-journal=joma no-vol=9 cd-vols= no-issue=9 article-no= start-page=1913 end-page=1915 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080515 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=CO oxidation on perovskite-type LaCoO3 synthesized using ethylene glycol and citric acid en-subtitle= kn-subtitle= en-abstract= kn-abstract=In order to synthesize perovskite-type LaCoO3 with good surface crystallinity, the gel prepared by adding both ethylene glycol (EG) and citric acid (CA) to the aqueous solution of La(NO3)3 center dot 6H(2)O and Co(NO3)(2) center dot 6H(2)O was fired at 600 degrees C in air for 3 h. The transmission electron microscopy (TEM) observation indicated that the particles of LaCoO3 tended to have a uniform shape at EG/CA = 4. Although, the specific surface area of LaCoO3 synthesized using both EG and CA was slightly smaller than that of LaCoO3 synthesized using only CA, the catalytic activity of CO oxidation became higher by adding EG. en-copyright= kn-copyright= en-aut-name=TaguchiHideki en-aut-sei=Taguchi en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamasakiSatoshi en-aut-sei=Yamasaki en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ItadaniAtsushi en-aut-sei=Itadani en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YosinagaMasashi en-aut-sei=Yosinaga en-aut-mei=Masashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HirotaKen en-aut-sei=Hirota en-aut-mei=Ken kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=The Graduate School of Natural Science and Technology (Science), Okayama University affil-num=2 en-affil= kn-affil=The Graduate School of Natural Science and Technology (Science), Okayama University affil-num=3 en-affil= kn-affil=The Graduate School of Natural Science and Technology (Science), Okayama University affil-num=4 en-affil= kn-affil=Kyoto Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence, JST affil-num=5 en-affil= kn-affil=Faculty of Engineering, Doshisha University en-keyword=LaCoO3 kn-keyword=LaCoO3 en-keyword=perovskite kn-keyword=perovskite en-keyword=sol-gel preparation kn-keyword=sol-gel preparation en-keyword=CO oxidation kn-keyword=CO oxidation END