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ID 33006
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Author
Arata, Noriko
Abstract
We produced a monoclonal antibody, named A20, which specifically recognizes a 35 kDa protein and stains myelinated axons in zebrafish brain. The A20 antigen is located at the outside of the myelin layer of large axons, and comprises a fine meshwork composed of thin unit fibers about 1–2 μm in length and about 100–200 nm in thickness. The unit fibers form pentagonal and hexagonal structures, which further polymerize into an envelope structure on the axons. The A20 monoclonal antibody did not stain neuronal cell bodies nor synapses. Instead, the distribution of the A20 antigen was along axons, practically coincident with the distribution of myelin basic protein. The monoclonal antibody stained only axons in the central nervous system (CNS), and not the extracellular matrix surrounding Schwann cells. These results suggest that this antigenic meshwork (which we call the periaxonal net) is synthesized by oligodendrocytes. During the development of the zebrafish brain, the periaxonal net appeared after the formation of myelin on the axons. The periaxonal net developed first at the brain stem, then gradually appeared at the caudal end of the spinal cord. The thickness of the periaxonal net around the Mauthner axon changed during development. Although the thickness of the Mauthner axon continues to grow throughout life, the thickness of periaxonal net stopped growing at 6 months after fertilization.
Keywords
Oligodendrocyte
Monoclonal antibody
Extracellular matrix
Central nervous system
Note
Digital Object Identifer:10.1016/S0006-8993(02)03888-X
Published with permission from the copyright holder. This is the author's copy, as published in Brain Research, 31 January 2003, Volume 961, Issue 2, Pages 179-189.
Publisher URL:http://dx.doi.org/10.1016/S0006-8993(02)03888-X
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Copyright © 2002 Elsevier Science B.V. Limited. All rights reserved.
Published Date
2003-01-31
Publication Title
Brain Research
Volume
volume961
Issue
issue2
Publisher
Elsevier Science B.V.
Start Page
179
End Page
189
ISSN
0006-8993
NCID
AA0057324X
Content Type
Journal Article
language
英語
Copyright Holders
Elsevier Science B.V.
File Version
author
Refereed
True
DOI
PubMed ID
Web of Sience KeyUT
Submission Path
biology_general/19