このエントリーをはてなブックマークに追加
ID 32993
FullText URL
Author
Deguchi, Shinji
Maeda, Kenjiro
Ohashi, Toshiro
Sato, Masaaki
Abstract
The mechanical contribution of nucleus in adherent cells to bearing intracellular stresses remains unclear. In this paper, the effects of fluid shear stress on morphology and elastic properties of endothelial nuclei were investigated. The morphological observation suggested that the nuclei in the cytoplasm were being vertically compressed under static conditions, whereas they were elongated and more compressed with a fluid shear stress of 2 Pa (20 dyn/cm(2)) onto the cell. The elongated nuclei remained the shape even after they were isolated from the cells. The micropipette aspiration technique on the isolated nuclei revealed that the elastic modulus of elongated nuclei, 0.62 +/- 0.15 kPa (n = 13, mean +/- SD), was significantly higher than that of control nuclei, 0.42 +/- 0.12 kPa (n = 11), suggesting that the nuclei remodeled their structure due to the shear stress. Based of these results and a transmission electron microscopy, a possibility of the nucleus as an intracellular compression-bearing organelle was proposed, which will impact interpretation of stress distribution in adherent cells. (C) 2005 Elsevier Ltd. All rights reserved.
Keywords
cell mechanics
nucleus
mechanical properties
shear stress
mechanotransduction
atomic-force microscopy
shear-stress
mechanical-properties
viscoelastic
properties
cells
Note
Digital Object Identifer:10.1016/j.jbiomech.2005.06.003
Published with permission from the copyright holder. This is the author's copy, as published in Journal of Biomechanics, September 2005, Volume 38, Issue 9, Pages 1751-1759.
Publisher URL:http://dx.doi.org/10.1016/j.jbiomech.2005.06.003
Direct access to Thomson Web of Science record
Copyright © 2005 Elsevier Ltd. All rights reserved.
Published Date
2005-09
Publication Title
Journal of Biomechanics
Volume
volume38
Issue
issue9
Publisher
Elsevier Science Ltd.
Start Page
1751
End Page
1759
Content Type
Journal Article
language
英語
Copyright Holders
Elsevier Ltd.
Refereed
True
DOI
Web of Sience KeyUT
Submission Path
biology_general/27