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ID 57315
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Saito, Nanami Okayama University, Research Core for Interdisciplinary Sciences (RCIS)
Nishimura, Koki Okayama University, Research Core for Interdisciplinary Sciences (RCIS)
Makanae, Aki Okayama University, Research Core for Interdisciplinary Sciences (RCIS)
Satoh, Akira Okayama University, Research Core for Interdisciplinary Sciences (RCIS) Kakenhi
Abstract
Gill regeneration has not been well studied compared to regeneration of other appendages, such as limb and tail regeneration. Here, we focused on axolotl gill regeneration and found that Fgf- and Bmp-signaling are involved in their gill regeneration mechanism. Axolotls have three pairs of gill rami, and each gill ramus has multiple gill filaments. The gills consist of mesenchyme rich in extracellular matrix and epidermis. The gill nerves are supplied from the trigeminal ganglia located in the head. Denervation resulted in no gill regeneration responses. Nerves and gills express Bmp and Fgf genes, and treating animals with Fgf- and Bmp-signaling inhibitors results in phenotypes similar to those seen in denervated gills. Inducing an accessory appendage is a standard assay in amphibian regeneration research. In our study, an accessory gill could be induced by lateral wounding, suggesting that thin axon fibers and mesenchymal Fgfs and Bmps contributed to the induction of the accessory structure. Such accessory gill induction was inhibited by the denervation. Exogenous Fgf2+Fgf8+Bmp7, which have been determined to function as a regeneration inducer in urodele amphibians, could compensate for the effects denervation has on accessory blastema formation. Our findings suggest that regeneration of appendages in axolotls is regulated by common Fgf- and Bmp-signaling cascades.
Keywords
Blastema induction
Bmp
Fgf
Gill regeneration
Nerve
Organ regeneration
Published Date
2019-08-15
Publication Title
Developmental Biology
Volume
volume452
Publisher
Elsevier
Start Page
104
End Page
113
ISSN
00121606
NCID
AA00627848
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2019 The Authors. Published by Elsevier Inc.
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Related Url
isVersionOf https://doi.org/10.1016/j.ydbio.2019.04.011
License
http://creativecommons.org/licenses/by-nc-nd/4.0/
Citation
Nanami Saito, Koki Nishimura, Aki Makanae, Akira Satoh, Fgf- and Bmp-signaling regulate gill regeneration in Ambystoma mexicanum, Developmental Biology, Volume 452, Issue 2, 2019, Pages 104-113, ISSN 0012-1606, https://doi.org/10.1016/j.ydbio.2019.04.011.
Funder Name
Japan Society for the Promotion of Science
助成番号
17H03685 : 四肢再生全般を支配する神経因子の解明と再生不能(不全)動物への応用
Open Access (Publisher)
OA
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Non-OpenArchive