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Akhter, Mst Nahid Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Hara, Emilio Satoshi Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kadoya, Koichi Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Okada, Masahiro Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID
Matsumoto, Takuya Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kaken ID researchmap
Abstract
Current stem cell-based techniques for bone-like tissue synthesis require at least two to three weeks. Therefore, novel techniques to promote rapid 3D bone-like tissue synthesis in vitro are still required. In this study, we explored the concept of using cell nanofragments as a substrate material to promote rapid bone formation in vitro. The methods for cell nanofragment fabrication were ultrasonication (30 s and 3 min), non-ionic detergent (triton 0.1% and 1%), or freeze-dried powder. The results showed that ultrasonication for 3 min allowed the fabrication of homogeneous nanofragments of less than 150 nm in length, which mineralized surprisingly in just one day, faster than the fragments obtained from all other methods. Further optimization of culture conditions indicated that a concentration of 10 mM or 100 mM of beta-glycerophosphate enhanced, whereas fetal bovine serum (FBS) inhibited in a concentration-dependent manner, the mineralization of the cell nanofragments. Finally, a 3D collagen-cell nanofragment-mineral complex mimicking a bone-like structure was generated in just two days by combining the cell nanofragments in collagen gel. In conclusion, sonication for three min could be applied as a novel method to fabricate cell nanofragments of less than 150 nm in length, which can be used as a material for in vitro bone tissue engineering.
Keywords
bone tissue engineering
cell nanofragments
dead cells
mineralization
osteogenesis
Published Date
2020-07-27
Publication Title
International Journal of Molecular Sciences
Volume
volume21
Issue
issue15
Publisher
MDPI
Start Page
5327
ISSN
1422-0067
NCID
AA12038549
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2020 by the authors.
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publisher
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.3390/ijms21155327
License
http://creativecommons.org/licenses/by/4.0/
Funder Name
Japan Society for the Promotion of Science
助成番号
JP18K17119
JP19H03837
JP20H04534
JP20H05225