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Masaki, Kento Department of Immunobiology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Hiraki, Yuhji Department of Immunobiology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Onishi, Hiroka Department of Immunobiology, Faculty of Pharmacy and Pharmaceutical Sciences, Okayama University
Satoh, Yuka Department of Immunobiology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Roche, Paul A. Experimental Immunology Branch, National Cancer Institute, National Institutes of Health
Tanaka, Satoshi Department of Pharmacology, Division of Pathological Sciences, Kyoto Pharmaceutical University
Furuta, Kazuyuki Department of Immunobiology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Abstract
In addition to antigen presentation to CD4(+)T cells, aggregation of cell surface major histocompatibility complex class II (MHC-II) molecules induces signal transduction in antigen presenting cells that regulate cellular functions. We previously reported that crosslinking of MHC-II induced the endocytosis of MHC-II, which was associated with decreased surface expression levels in murine dendritic cells (DCs) and resulted in impaired activation of CD4(+)T cells. However, the downstream signal that induces MHC-II endocytosis remains to be elucidated. In this study, we found that the crosslinking of MHC-II induced intracellular Ca(2+)mobilization, which was necessary for crosslinking-induced MHC-II endocytosis. We also found that these events were suppressed by inhibitors of Syk and phospholipase C (PLC). Treatments with a phorbol ester promoted MHC-II endocytosis, whereas inhibitors of protein kinase C (PKC) suppressed crosslinking-induced endocytosis of MHC-II. These results suggest that PKC could be involved in this process. Furthermore, crosslinking-induced MHC-II endocytosis was suppressed by inhibitors of clathrin-dependent endocytosis. Our results indicate that the crosslinking of MHC-II could stimulate Ca(2+)mobilization and induce the clathrin-dependent endocytosis of MHC-II in murine DCs.
Keywords
MHC-II
dendritic cells
endocytosis
crosslink
PKC
Published Date
2020-07-30
Publication Title
Cells
Volume
volume9
Issue
issue8
Publisher
MDPI
Start Page
1810
ISSN
2073-4409
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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© 2020 by the authors.
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isVersionOf https://doi.org/10.3390/cells9081810
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http://creativecommons.org/licenses/by/4.0/