このエントリーをはてなブックマークに追加
ID 60761
FullText URL
Author
Akıl, Caner Institute of Molecular and Cell Biology, Agency for Science, Technology and Research
Tran, Linh T. Research Institute for Interdisciplinary Science, Okayama University
Orhant-Prioux, Magali CytomorphoLab, Interdisciplinary Research Institute of Grenoble
Baskaran, Yohendran aInstitute of Molecular and Cell Biology, Agency for Science, Technology and Research
Manser, Edward aInstitute of Molecular and Cell Biology, Agency for Science, Technology and Research
Blanchoin, Laurent CytomorphoLab, Interdisciplinary Research Institute of Grenoble
Robinson, Robert C. cResearch Institute for Interdisciplinary Science, Okayama University ORCID Kaken ID researchmap
Abstract
Asgard archaea genomes contain potential eukaryotic-like genes that provide intriguing insight for the evolution of eukaryotes. The eukaryotic actin polymerization/depolymerization cycle is critical for providing force and structure in many processes, including membrane remodeling. In general, Asgard genomes encode two classes of actin-regulating proteins from sequence analysis, profilins and gelsolins. Asgard profilins were demonstrated to regulate actin filament nucleation. Here, we identify actin filament severing, capping, annealing and bundling, and monomer sequestration activities by gelsolin proteins from Thorarchaeota (Thor), which complete a eukaryotic-like actin depolymerization cycle, and indicate complex actin cytoskeleton regulation in Asgard organisms. Thor gelsolins have homologs in other Asgard archaea and comprise one or two copies of the prototypical gelsolin domain. This appears to be a record of an initial preeukaryotic gene duplication event, since eukaryotic gelsolins are generally comprise three to six domains. X-ray structures of these proteins in complex with mammalian actin revealed similar interactions to the first domain of human gelsolin or cofilin with actin. Asgard two-domain, but not one-domain, gelsolins contain calcium-binding sites, which is manifested in calcium-controlled activities. Expression of two-domain gelsolins in mammalian cells enhanced actin filament disassembly on ionomycin-triggered calcium release. This functional demonstration, at the cellular level, provides evidence for a calcium-controlled Asgard actin cytoskeleton, indicating that the calcium-regulated actin cytoskeleton predates eukaryotes. In eukaryotes, dynamic bundled actin filaments are responsible for shaping filopodia and microvilli. By correlation, we hypothesize that the formation of the protrusions observed from Lokiarchaeota cell bodies may involve the gelsolin-regulated actin structures.
Keywords
actin
gelsolin
Asgard archaea
eukaryogenesis
X-ray crystallography
Published Date
2020-08-18
Publication Title
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Start Page
117
End Page
33
ISSN
0027-8424
NCID
AA10808769
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
Copyright © 2020 the Author(s)
File Version
publisher
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1073/pnas.2009167117
License
https://creativecommons.org/licenses/by-nc-nd/4.0/
Funder Name
Japan Society for the Promotion of Science
助成番号
JP20H00476