このエントリーをはてなブックマークに追加
ID 46909
FullText URL
Author
Furumatsu, Takayuki Kakenhi
Ozaki, Toshifumi Kakenhi
Asahara, Hiroshi
Abstract
Transforming growth factor (TGF)-β has an essential role for the Sry-type high-mobility-group box (Sox)-regulated chondrogenesis. Chondrogenic differentiation is also controlled by chromatin-mediated transcription. We have previously reported that TGF-β-regulated Smad3 induces chondrogenesis through the activation of Sox9-dependent transcription. However, the cross-talk between TGF-β signal and Sox9 on chromatin-mediated transcription has not been elucidated. In the present study, we investigated the activity of Smad3, Sox9, and coactivator p300 using an in vitro chromatin assembly model. Luciferase reporter assays revealed that Smad3 stimulated the Sox9-mediated transcription in a TGF-β-dependent manner. Recombinant Sox9 associated with phosphorylated Smad3/4 and recognized the enhancer region of type II collagen gene. In vitro transcription and S1 nuclease assays showed that Smad3 and p300 cooperatively activated the Sox9-dependent transcription on chromatin template. The combination treatment of phosphorylated Smad3, Sox9, and p300 were necessary for the activation of chromatin-mediated transcription. These findings suggest that TGF-β signal Smad3 plays a key role for chromatin remodeling to induce chondrogenesis via its association with Sox9.
Keywords
Chondrogenesis
Chromatin
p300
Smad3
Sox9
Published Date
2009-05
Publication Title
The International Journal of Biochemistry & Cell Biology
Volume
volume41
Issue
issue5
Publisher
Pergamon-Elsevier Science Ltd.
Start Page
1198
End Page
1204
ISSN
1357-2725
NCID
AA11036038
Content Type
Journal Article
language
英語
Copyright Holders
© 2008 Elsevier Ltd. All rights reserved.
File Version
author
Refereed
True
DOI
PubMed ID
Web of Sience KeyUT