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ID 54743
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Author
Takahashi Nobumasa Department of Paediatrics, Fukuyama Medical Centre
Nojima, Ikuko Department of Paediatrics, Fukuyama Medical Centre
Araki, Tooru Department of Paediatrics, Fukuyama Medical Centre
Takasugi, Mizue Department of Paediatrics, Fukuyama Medical Centre
Sakane, Tomoko Department of Paediatrics, Fukuyama Medical Centre
Kodera, Aya Department of Paediatrics, Fukuyama Medical Centre
Ikeda, Masanori Department of Paediatrics, Fukuyama Medical Centre
Tsukahara, Hirokazu Department of Paediatrics, Okayama University Hospital Kaken ID researchmap
Abstract
Objectives: To compare the diagnostic performance of two norovirus rapid immunochromatographic kits (QuickNavi(®)-Norovirus [QN] and QuickNavi®-Norovirus 2 [QN2]; Denka Seiken, Niigata, Japan) for neonatal and infant faecal specimens.
Methods: Monthly faecal samples were collected from infants from birth to 12 months of age, and tested for norovirus using QN and QN2. Real-time reverse transcription polymerase chain reaction (RT-PCR) was used as the gold standard for norovirus detection. The diagnostic performance of the kits was calculated.
Results: A total of 343 specimens from 81 infants were analysed. In all samples, the specificity of QN and QN2 was 80% (275/343) and 99% (339/343), respectively. In infants aged <1 month, the specificity of QN was 33% (23/70), increasing to 93% at 4 months of age. Specificity of QN2 was ≥94% in infants between 0 and 12 months of age.
Conclusions: QN2 offers improved performance and is more useful than QN for the diagnosis of norovirus infection in the neonatal and infant period.
Keywords
Norovirus
false positive
immunochromatography
neonate
rapid detection test
specificity
Published Date
2015-09-03
Publication Title
Journal of International Medical Research
Volume
volume43
Issue
issue5
Publisher
Sage
Start Page
648
End Page
652
ISSN
0300-0605
NCID
AA00700686
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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publisher
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1177/0300060515592902
Citation
Takahashi N, Nojima I, Araki T, et al. Evaluation of rapid immunochromatographic tests for norovirus in neonatal and infant faecal specimens. J Int Med Res. 2015;43(5):648‐652. doi:10.1177/0300060515592902