このエントリーをはてなブックマークに追加
ID 60852
FullText URL
Author
Kondo, Hideki Research Institute for Bioresources, Okayama University Kaken ID publons researchmap
Maeda, Takanori College of Bioresource Sciences, Nihon University
Tamada, Tetsuo Research Institute for Bioresources, Okayama University ORCID Kaken ID researchmap
Abstract
Orchid fleck virus (OFV) has a bipartite negative-sense RNA genome with sequence similarities to plant rhabdoviruses. The non-enveloped bullet-shaped particles of OFV are similar to those of the internal ribonucleoprotein (RNP)-M protein structure of rhabdoviruses, but they are about half the size of typical plant rhabdoviruses. Purified preparations contained intact bullet-shaped and filamentous particles. The filamentous particles showed a tightly coiled coil structure or a coiled structure with a helical twist, which resembles the RNP complex of rhabdoviruses. OFV bullet-shaped particles were structurally stable in solutions containing 2% Triton X-100 and 0.8 M NaCl. Western blot analyses revealed that the bullet-shaped particles contained N, P and M proteins, while filamentous particles contained mainly N and P proteins. In addition, a small amount of the L protein was detected in both types of particles. Thus, the structural proteins of OFV have properties similar to those of rhabdoviruses, except that the particles are non-enveloped and are relatively resistant to detergent-treatment under high-salt conditions.
Keywords
Rabies
ORF4 Protein
Sucrose Density Gradient Centrifugation
Coil Coil Structure
Potential Glycosylation Site
Note
This is a post-peer-review, pre-copyedit version of an article published in Archives of Virology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00705-008-0268-6.
Published Date
2008-12-06
Publication Title
Archives of Virology
Volume
volume154
Issue
issue1
Publisher
Springer
Start Page
37
End Page
45
ISSN
0304-8608
NCID
AA00548901
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
File Version
author
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1007/s00705-008-0268-6