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ID 16543
Eprint ID
16543
フルテキストURL
Thumnail 93_361.pdf 4.78 MB
タイトル(別表記)
Quantitative Studies on Bleomycin-Induced Strand Breakage of Chromatin DNA
著者
片岡 和博 岡山大学医学部癌源研究施設生化学部門
抄録
Single and double strand breakage of DNA by bleomycin under various conditions was analyzed quantitatively using purified simian virus 40 (SV40) DNA and SV40 chromatin. The method employed for DNA assay was fluorometry of SV40 DNA separated into three forms I, II, and III by 1.4% agarose gel electrophoresis and stained with ethidium bromide. This method was extremely simple and accurate for quantitative estimation of single and double strand breakage of circular DNA. The amount of bleomycin required for in vitro breakage of SV40 chromatin DNA was much higher than that of naked SV40 DNA. 2-Mercaptoethanol accerated bleomycin-induced DNA strand breakage. The concentration of 2-mercaptoethanol required for the acceleration was higher in SV40 chromatin than in SV40 DNA. DNA strand breakage was induced by ferrous ions in the absence of bleomycin to a similar level in SV40 chromatin and in SV40 DNA. The acceleration of bleomycin-induced strand breakage by a low concentration of ferrous ion was higher in SV40 DNA than in SV40 chromatin. Ethylenediaminetetraacetic acid (EDTA) and copper ions were not efficient for stopping the reaction of bleomycin-induced DNA breakage. Sodium dodecyl sulfate(SDS) was a useful reagent for stopping the reaction.
キーワード
ブレオマイシン
SV40
クロマチン
DNA鎖切断
ゲル電気泳動
発行日
1981-04-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
93巻
3-4号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
361
終了ページ
381
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/93/3-4/93_3-4_361/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
OAI-PMH Set
岡山大学
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Sort Key
10
Eprints Journal Name
joma