ID 30753
JaLCDOI
フルテキストURL
著者
Nakatani, Satoru Okayama University
Naito, Ichiro Okayama University
Momota, Ryusuke Okayama University
Hinenoya, Noriko Okayama University
Horiuchi, Kanji Okayama University
Nishida, Keiichiro Okayama University
Ohtsuka, Aiji Okayama University
抄録

We attempted to prepare colloidal iron within tissues by means of microwave irradiation. Mouse tissue blocks were fixed with a mixture of paraformaldehyde and ferric chloride in a cacodylate buffer, immersed in a cacodylate buffered ferric chloride solution, and irradiated in a microwave processor. Colloidal iron was prepared within tissues or cells, and was observed in the form of electron dense fine granules (1-2 nm in diameter) by transmission electron microscopy. Collagen fibrils in the connective tissue showed colloidal iron deposition at regular periodical intervals. Cells in the splenic tissue showed that fine colloidal granules were deposited on the ribosomes but not on the nuclear chromatin. This finding suggests that ferric ions could not diffuse into the nucleus, which was surrounded by the nuclear envelope. The podocyte processes of the renal glomerulus were stained diffusedly. Though this microwave in situ colloidal iron preparation method has some limitations, it is convenient for use in biomedical specimen preparation in transmission electron microscopy.

キーワード
colloidal iron
microwave
histochemistry
transmission electron microscopy
Amo Type
Article
発行日
2006-02
出版物タイトル
Acta Medica Okayama
60巻
1号
出版者
Okayama University Medical School
開始ページ
59
終了ページ
64
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT
過去1年のアクセス数
アクセス数 : ?
ダウンロード数 : ?

今月のアクセス数
アクセス数 : ?
ダウンロード数 : ?