ID 32620
JaLCDOI
フルテキストURL
Thumnail fulltext.pdf 1.12 MB
著者
Takata, Hiroshi Okayama University
Yoshino, Tadashi Okayama University
Hoshida, Yoshihiko Okayama Univeristy
Takata, Ikuko Okayama University
Akagi, Tadaatsu Okayama University
抄録

A cell line of human lung large cell carcinoma (LCC) was established directly from the metastatic skin tumor tissue. The clinical course of the patient who carried this carcinoma was peculiar; generalized lymphadenopathy, histologically resembling Hodgkin's disease, was found as the first clinical symptom. The lung tumor was not discovered until the time of autopsy. This cell line (KaMi) grew adherent to culture vessels with the population doubling time of 20.6h, formed colonies in soft agars with efficiency of 22.6%, and formed tumors in athymic nude mice. The authenticity of KaMi was confirmed by chromosomal analysis and isoenzyme patterns. KaMi cells bore a strong resemblance to the original tumor cells which were composed of small spindle cells, large polygonal cells, and multinucleated giant cells. Immunohistochemically, KaMi cells showed a weak tendency to differentiate to squamous cells, and these immunohistochemical reactivities were almost compatible to those of the original tumor cells, but ultrastructurally, KaMi cells were more immature than the original ones. Treatment with several reagents could not augment a differentiation of KaMi cells. Cytokeratin profiles showed a tendency of squamous cell differentiation. KaMi cells may aid in elucidating the pathogenesis and biology of LCC and its relationship to other lung tumors.

キーワード
Large cell lung carcinoma
cell line
cytokeratin
Amo Type
Article
発行日
1992-08
出版物タイトル
Acta Medica Okayama
46巻
4号
出版者
Okayama University Medical School
開始ページ
257
終了ページ
264
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT
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