Journal of Okayama Medical Association
Published by Okayama Medical Association

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Full-text articles are available 3 years after publication.

Ehrlich腹水癌細胞およびAdriamycin耐性細胞のRhodamine 123 uptakeを指標としたミトコンドリア活性

岡崎 良夫 岡山大学医学部放射線医学教室
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抄録
The positively charged fluorescent dye Rhodamine 123 (Rho 123) accumulates in mitochondria. An adriamycin (ADR)-resistant cell line derived from Ehrlich ascites tumor cells (wild EATC) was established in our laboratory. Overall mitochondrial activity of wild EATC and ADR-resistant EATC was investigated by the staining method with Rho 123. The uptake of Rho 123 into living cells was analyzed by flow cytometry. The intracellular Rho 123 uptake of ADR-resistant EATC was lower than that of wild EATC. The intracellular Rho 123 uptake of wild EATC increased when cells were treated with cepharanthine (CP). The intracellular Rho 123 uptake of ADR-resistant EATC increased markedly by CP treatment. ADR-resistant EATC cosumed more endogenous oxygen than wild EATC. The energy level of ADR-resistant EATC was discussed.
キーワード
アドリアマイシン (ADR)
ADR耐性細胞
ミトコンドリア
Rhodamine 123
ハイパーサーミア
ISSN
0030-1558
NCID
AN00032489