Journal of Okayama Medical Association
Published by Okayama Medical Association

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Full-text articles are available 3 years after publication.

タイプ1線毛保有大腸菌のレセプター認識能の多様性の解析

小林 直樹 岡山大学医学部細菌学教室
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抄録
The role of type 1 fimbriae has well been discussed for analyzing the mechanisms of infectious diseases of the urinary tract. In this study, the ability and variety of mannose-receptor recognition in 92 strains of clinically isolated Escherichia coli were examined using various mannose-carriers such as guinea pig red blood cells, Saccharomyces cerevisiae cells and two carriers, latex and liposome. Type 1 fimbriated E. coli showed a variety of reactivity to the receptors on different mannose-carriers. Effects of mannose-carriers and inhibitors in agglutination assays were examined using 48 strongly haemagglutinable strains. In the recognition of Man (1-2) Man-carriers, a high affinity to pNPα-D-mannose was observed in the highly reactive strains to the receptor on liposomes. The MIC ratios of D-mannose to pNPα-D-mannose significantly correlated to the reactivities of these strains to the receptor on the liposome. By the values of MIC ratio, these strains could be classified into two groups ; reactive and non-reactive strains. In the recognition of Man (1-3) Man-carriers, MIC ratios of D-mannose to pNPα-D-mannose differed among more highly reactive strains to Man (1-3) Man-latex, and AUT strains showed stronger reactivity and lower MIC ratio than AUC and ABP strains. The adhesins of type 1 fimbriae were divided into at least 4 type (α, β, γ and δ).
キーワード
Type 1 fimbriae
Adhesin
Receptor recognition
Urinary tract infection
ISSN
0030-1558
NCID
AN00032489