The effect of UV-B radiation on immune responses was evaluated by radiation of rat spleen and skin epidermal cells in vitro. The radiation deteriorated the immune responses without influencing the viability of the irradiated cells. The mitogenic blastognesis of the spleen cells was inhibited. The stimulatory effect of the spleen and skin cells was inhibuted in mixed lymphocyte cultures. The cytotoxicity of spleen cells was decrased. The susceptibility of target skin cells to natural cytotoxicuity was decreased, Therefore, UV-B radiation causes changes in the cell membrane resulting in the inhibition of immune responses.