Journal of Okayama Medical Association
Published by Okayama Medical Association

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Full-text articles are available 3 years after publication.

顆粒球コロニー刺激因子の同種免疫応答に及ぼす影響

上山 聰 岡山大学医学部第一外科学教室
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抄録
The administration of recombinant human granulocyte colony-stimulating factor (rhG-CSF) increased weight and cellularity of the spleen, leukocyte and granulocyte count of the peripheral blood in a dose- and duration-dependent fashion in mice. When spleen cells as well as serum from mice treated with intraperitoneal injection of 200μg/㎏ of rhG-CSF daily for 7 days were added to an allogeneic mixed lymphocyte reaction (MLR) culture, MLR was significantly suppressed nonspecifically and in a dose-dependent fashion. MLR was also suppressed by adding supernatant of spleen cells using transwells, indicating that suppression was mediated by soluble factor (s) secreted from the spleen cells. The producer cells of the inhibiting factor were found in more mature granulocytes with a high density in the spleen. These results suggested that the proliferated granulocytes induced by G-CSF produced and released factor (s) that suppress allogeneic immune responses.
キーワード
顆粒球コロニー刺激因子(granulocyte colony-stimulating factor ; G-CSF)
同種免疫応答
免疫抑制
リンパ球混合反応
備考
原著
ISSN
0030-1558
NCID
AN00032489