There were known that the much production of ascites would be observed by the inoculation of the tsutsugamushi disease rickettsia to the chlorpromazine injected guinea pig. But there were still suspended the problem on the factors to result in much production of ascites. The author studies the relationships between the production of ascites and body weight of animal and between the former and breeding environment of animal, especially the temperature of environment. As the results of this study, it could be confirmed on animals having body weight 300-500 gms. that the body weight of animal had no relation with the production of ascites and the proliferation of the rickettsia in the animal so far as the animal was injected previously chlorpromazine; however, the temperature of environment affected powerfully to those. Namely, the production of ascites, the proliferation of the rickettsia and the presence of the specific cells for infection were found to be much on the infection in spring, fall and even in winter if the temperature kept at 15°C, but these evidence were not found on the infection in summer. These informations were thought to be valuable in order to obtain the tsutsugamushi disease rikettsia in large amount.