Journal of Okayama Medical Association
Published by Okayama Medical Association

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Full-text articles are available 3 years after publication.

全身性エリテマトーデスにおけるalternative pathwayに関する研究 ウサギ赤血球溶血反応を利用して 第1編 alternative pathway総溶血活性, properdin活性, Factor B活性測定法の基礎的考察

宮島 啓人 岡山大学医学部第三内科学教室
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抄録
Methods of measuring the activity of the total alternative complement pathway, properdin and Factor B with rabbit red blood cells(RaRBC) were examined. The following results were obtained. 1) 0.03M EGTA-GVB permitted activation of the alternative complement pathway without activation of the classical complement pathway. 2) RaRBC were hemolysed by C4/C2 depleted serum as well as by normal serum. RaRBC were not hemolysed by inulin or zymosan treated serum. 3) The activity of the total alternative complement pathway was measured with 50 % hemolysis of RaRBC in EGTA-GVB, designated as ACH50. 4) The activity of properdin or Factor B was measured with RaRBC and normal fresh human serum deficient in properdin (RP) or deficient in Factor B (RB).
キーワード
全身性エリテマトーデス
補体
alternative pathway総溶血活性
propendin活性
Factor B活性
ISSN
0030-1558
NCID
AN00032489