The formative mechanism of debrominated compounds was investigeted. The excretion rate of tribromobenzene metabolites into the rat urine was enhanced by pretreatment with phenobarbital, and the amount of debrominated bromophenol in urine was greater than that of sulfur containing compounds after phenobarbital pretreatment. on the other hand, sulfur containing compounds were predominant in the urine of rats pretreated with glutathione. Incubation of tribromobenzene with the 10,000g supernatant of rat liver homogenate from rats pretreated with phenobarbital resulted in an increase in 3.5-dibromophenol. The above effect was accelerated remarkably by NADH and NADPH. These results demonstrate two metabolic pathways in the debromination of tribromobenzene: one to sulfur containing compounds and another to bromophenol bodies.