Published by Misasa Medical Center, Okayama University Medical School
Published by Misasa Medical Center, Okayama University Medical School

<Formerly known as>
岡大三朝分院研究報告 (63号-72号) 環境病態研報告 (57号-62号)
岡山大学温泉研究所報告 (5号-56号) 放射能泉研究所報告 (1号-4号)

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Frizzled Activation by Wnt-l Is Required for beta -Catenin -T Cell Factor Dependent Transcription in Esophageal Cancer

水島 孝明 岡山大学大学院医歯学研究科生体情報医学, 老年医学
越智 浩二 岡山大学大学院医歯学研究科生体情報医学, 老年医学
江盛 康之 岡山大学大学院医歯学研究科生体情報医学, 老年医学
谷岡 洋亮 岡山大学大学院医歯学研究科生体情報医学, 老年医学
澤 公成 岡山大学大学院医歯学研究科生体情報医学, 老年医学
藤岡 真一 岡山大学大学院医歯学研究科生体情報医学, 老年医学
真治 紀之 岡山大学大学院医歯学研究科生体情報医学, 老年医学
小出 典男 岡山大学大学院医歯学研究科生体情報医学, 老年医学
谷崎 勝朗 岡山大学大学院医歯学研究科生体情報医学, 老年医学
抄録
Although, accumulation of nuclear and cytoplasmic beta-catenin has been observed in ESCCs, mutation of APC and beta-catenin are not found in ESCCs. Therefore, another mechanism for cytoplasmic beta- catenin accumulation might exist in ESCCs. Materials and Methods: Human ESCCs cell lines and the 293 stable transfectants expressing Wnt-1, Wnt-5A, and Wnt-7A were cultured under standard conditions. The TOPFlash or FOPFlash reporter plasmids were transfected. Results: Transfected mutant beta-catenin as well as an axin fragment harbing the GSK3 beta interaction domain, the latter a potent GSK3 beta inhibitor. both robustly activated pTOPFlash in ESCCs cells. When pTOPFlash/pFOPFlash reporters were transfected in ESCCs cell lines followed by cocultivation with 293 cells that stably express Wnt -1, all cell lines except one demonstrated TCF mediated transcriptional. But, cells were co- cultured, Wnt - 7A or Wnt - 5A did not activate TCF mediated transcription in a cell number dependent fashion. Discussion; We report the activation of TCF promoter gene by extemal Wnt stimuli in ESCCs cells.
キーワード
esophageal carcinoma
Wnt
TCF
beta-catenin
ISSN
1348-1258
NCID
AA11840279