Scientific Reports of the Faculty of Agriculture, Okayama University
Published by the Faculty of Agriculture, Okayama University
ONLINE ISSN : 2186-7755

Ultrastructure of Ribosomes in Cells of The Tobacco Leaves Treated With Cycloheximide

古沢 巌 Department of Plant Science, University of Alberta
大内 成志 岡山大学
抄録
The effect of cycloheximide on cell organelles was investigated at the level of ultrastructure, with an emphasis on the ribosomal behaviors. The antibiotic, at low concentrations, primarily caused an orderly alignment of ribosomes on endoplasmic reticuli (ER), giving rise to rough-surfaced ER. Development of membrane systems was also a characteristic feature of the cells treated at low concentration or incubated for short period of time. Prolonged incubation or treatment at higher concentrations led eventuaily to the degeneration of cytoplasmic and nuclar organizations, the aggregation of ribosomal debris being one of the conspicuous features. On the contrary, no major structural change was observed of the chloroplasts and mitochondria which are both resistant to the inhibitory action of cycloheximide. These results were discussed in relation to the inhibition of protein synthesis by the cycloheximide.
ISSN
0474-0254
NCID
AN00033029