Streptomyces hygroscopicus subsp. limoneus JCM 4911 , a validamycin producer was found to produce a volatile in the validamycin producing medium . The volatile was produced only by the organism which could form abundant aerial mycelium during the growth phase for one to two days of the culture and, thereafter, it decreased. Maximum production (3.88μg/ml) of the volatile was found in two-days' cultured broth with the conditions for validamycin production. On the contrary, the validamycin production started at around two days into the culture and increased up to five to seven days of the culture with maximum production of 440μg/ml. The volatile was extracted with n-pentane from the culture broth of S. hygroscopicus subsp. limoneus JCM 4911 and was identical with n-hexanol by GC-MS analysis.