著者 二宮 喜文|
発行日 1997-10
出版物タイトル 岡山実験動物研究会報
14巻
資料タイプ その他
著者 Demircan, Kadir| Hirohata, Satoshi| Nishida, Keiichiro| Hatipoglu, Omer F.| Oohashi, Toshitaka| Yonezawa, Tomoko| Apte, Suneel S.| Ninomiya, Yoshifumi|
発行日 2005-5
出版物タイトル Arthritis & Rheumatism
52巻
5号
資料タイプ 学術雑誌論文
著者 Fleischmajer, Raul| Utani, Atsushi| Douglas MacDonald II, E.| Perlish, Jerome S| Pan, Te-Cheng| Chu, Mon-Li| Nomizu, Motoyoshi| Ninomiya, Yoshifumi| Yamada, Yoshihiko|
発行日 1998-7
出版物タイトル Journal of Cell Science
111巻
14号
資料タイプ 学術雑誌論文
著者 Pöschl, Ernst| Schlötzer-Schrehardt, Ursula| Brachvogel, Bent| Saito, Kenji| Ninomiya, Yoshifumi| Mayer, Ulrike|
発行日 2004-04
出版物タイトル Development
131巻
7号
資料タイプ 学術雑誌論文
著者 Matsuura, Hiroko| Murakami, Takashi| Hina, Kazuyoshi| Yamamoto, Keizo| Kawamura, Hiroshi| Sogo, Taiji| Shinohata, Ryoko| Usui, Shinichi| Ninomiya, Yoshifumi| Kusachi, Shozo|
発行日 2008-02
出版物タイトル Clinical Biochemistry
41巻
3号
資料タイプ 学術雑誌論文
著者 Momota, Ryusuke| Naito, Ichiro| Ninomiya, Yoshifumi| Ohtsuka, Aiji|
発行日 2011-05
出版物タイトル Matrix Biology
30巻
4号
資料タイプ 学術雑誌論文
著者 別宮 洋子| 二宮 善文| 大橋 俊孝|
発行日 2012-04-01
出版物タイトル 岡山医学会雑誌
124巻
1号
資料タイプ 学術雑誌論文
著者 Obika, Masanari| Ogawa, Hiroko| Takahashi, Katsuyuki| Li, Jiayi| Hatipoglu, Omer Faruk| Cilek, Mehmet Zeynel| Miyoshi, Toru| Inagaki, Junko| Ohtsuki, Takashi| Kusachi, Shozo| Ninomiya, Yoshifumi| Hirohata, Satoshi|
発行日 2012-10
出版物タイトル Cancer Science
103巻
10号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/53521
フルテキストURL 69_3_145.pdf
著者 Ishii, Hiroko| Kamikawa, Shigeshi| Hirohata, Satoshi| Mizutani, Akifumi| Abe, Koji| Seno, Masaharu| Oohashi, Toshitaka| Ninomiya, Yoshifumi|
抄録 Eosinophil cationic protein (ECP) is well known as a cationic protein contained in the basic granules of activated eosinophils. Recent studies have reported that ECP exhibits novel activities on various types of cells, including rat neonatal cardiomyocytes. Here we evaluated the effects of ECP on rat cardiac myoblast H9c2 cells. Our results showed that ECP enhanced the survival of the cells, in part by promoting the ERK and Akt/GSK-3β signaling pathways. ECP attenuated the cytotoxic effects of H2O2 on H9c2 cells as well as the production of reactive oxygen species, the number of apoptotic cells and caspase 3/7 activity in the cells. In conclusion, ECP activated the ERK and Akt/GSK-3β pathways, resulting in anti-oxidative effects on H9c2 cells that attenuated apoptosis.
キーワード ECP reactive oxygen species Akt ERK
Amo Type Original Article
発行日 2015-06
出版物タイトル Acta Medica Okayama
69巻
3号
出版者 Okayama University Medical School
開始ページ 145
終了ページ 153
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2015 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 26101190
Web of Sience KeyUT 000356903000003
JaLCDOI 10.18926/AMO/31831
フルテキストURL fulltext.pdf
著者 Hatipoglu, Omer Faruk| Hirohata, Satoshi| Yaykasli, Kursat Oguz| Cilek, Mehmet Zeynel| Demircan, Kadir| Shinohata, Ryoko| Yonezawa, Tomoko| Oohashi, Toshitaka| Kusachi, Shozo| Ninomiya, Yoshifumi|
抄録 <p>ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1) is an inflammatory-induced gene. We have previously reported that ADAMTS1 was strongly but transiently expressed in the infarcted heart. In this study, we investigated whether a 3'-untranslated region (UTR) affects the mRNA stability of this gene. When stimulated with tissue necrosis factor (TNF)-alpha, the expression level of ADAMTS1 mRNA rapidly increased, but the induction of ADAMTS1 mRNA peaked at 6h after stimulation, after which the expression levels of ADAMTS1 mRNA decreased. The 3'-UTR ADAMTS1 mRNA contains multiple adenine and uridine-rich elements, suggesting that the 3'-UTR may regulate gene stability. The addition of actinomycin D, an RNA synthesis inhibitor, demonstrated the decay of induced ADAMTS1 mRNA by TNF-alpha. Furthermore, a region containing multiple AUUUA motifs within the ADAMTS1 3'-UTR destabilized transfected Enhanced Green Fluorescence Protein (EGFP) mRNA expression. These results demonstrated that the ADAMTS1 3'-UTR may regulate the expression of ADAMTS1 mRNA.</p>
キーワード ADAMTS1 gene regulation metalloproteinase
Amo Type Original Article
発行日 2009-04
出版物タイトル Acta Medica Okayama
63巻
2号
出版者 Okayama University Medical School
開始ページ 79
終了ページ 85
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 19404339
Web of Sience KeyUT 000265457600002
JaLCDOI 10.18926/AMO/31728
フルテキストURL fulltext.pdf
著者 Nomoto, Hiroyuki| Oohashi, Toshitaka| Hirakawa, Satoshi| Ueki, Yasuyoshi| Ohtsuki, Hiroshi| Ninomiya, Yoshifumi|
抄録 <p>We herein determined by fluorescence in situ hybridization the chromosomal localization of 2 human genes, BRAL1 and BCAN, both of which belong to the link-module superfamily, i.e. to the same band of chromosome 1q21-23. Further analysis of the genomic organization of BRAL1 and BCAN revealed that the BRAL1 gene was located 20-kb upstream of the BCAN start site. We isolated a polymorphic dinucleotide (CA) repeat sequence from a genomic clone containing the BCAN gene. High heterozygosity (0.79) makes this polymorphism a useful marker in the study of genetic disorders. Knowledge of the structure of the genes and the marker provides essential information for further analysis of the gene locus at chromosome 1q21-23.</p>
キーワード BRAL1 BCAN FISH schizophrenia polymorphic marker
Amo Type Article
発行日 2002-02
出版物タイトル Acta Medica Okayama
56巻
1号
出版者 Okayama University Medical School
開始ページ 25
終了ページ 29
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 11873941
Web of Sience KeyUT 000174031300005
JaLCDOI 10.18926/AMO/32300
フルテキストURL fulltext.pdf
著者 Nakamura, Koki| Irie, Hiroyuki| Fujisawa, Emi| Yoshioka, Hidekatsu| Ninomiya, Yoshifumi| Sakuma, Isao| Sano, Shunji|
抄録 <p>While heat shock protein (HSP) 72 is known as a stress protein, there have been no reports of HSP 72 expression in patients who have undergone surgery for congenital heart disease. Fourteen patients (7 males and 7 females) who had undergone surgery for congenital heart disease were studied. The ages of the patients ranged from 2 months to 43 years old (mean 6.5 +/- 10.8 years old; median 3.0 years old). The diagnoses were Tetralogy of Fallot in seven, pulmonary atresia with ventricular septal defect (VSD) in three, complex anomalies in three, and VSD in one patient. Histological study and HSP analysis using Western blots and immunostaining with anti-HSP 72 monoclonal antibody were performed for right ventricular muscle samples resected during the surgery. The histological findings showed hypertrophic changes of ventricular cardiomyocytes in all samples studied. Western blots detected HSP 72 expression of various degrees in all specimens. Immunostaining using monoclonal antibody against HSP 72 showed that the protein was present in the nuclei and cytoplasm of cardiomyocytes. In conclusion, although it is difficult to determine the cause of the &#34;stress&#34; that triggers HSP 72 expression in cardiomyocytes, low O2 saturation and pressure overload might act as a &#34;stress&#34;, and the only common factor that induced HSP 72 in every sample was hypertrophy.</p>
キーワード heat shock protein 72 (HSP 72) human heart congentional cardiac surgery hypertrophy
Amo Type Article
発行日 2000-06
出版物タイトル Acta Medica Okayama
54巻
3号
出版者 Okayama University Medical School
開始ページ 103
終了ページ 109
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 10925734
Web of Sience KeyUT 000087965700002
JaLCDOI 10.18926/AMO/30509
フルテキストURL fulltext.pdf
著者 Yanai, Hiroyuki| Yoshino, Tadashi| Takahashi, Kiyoshi| Ninomiya, Yoshifumi| Akagi, Tadaatsu|
抄録 <p>Circulating hepatitis C virus (HCV) particles can be fractionated by means of differential flotation centrifugation. It is reported that in the bottom fraction HCV is in the form immune complexes, whereas in the top, it is free of antibodies. We evaluated the significance of circulating complex and free HCV in chronic hepatitis C, and assessed the relationship in terms of the response to interferon (IFN) therapy. We examined sera before, just after, and 1 year after administering IFN to 18 patients with chronic hepatitis C, 10 of whom responded (group CR), and 8 did not (group NR). The amounts of virus were similar between both groups before therapy. After differential flotation centrifugation with 1.063 g/ml of NaCl, the top and bottom fractions were assayed for HCV RNA. Before therapy, HCV RNA was detected in the top fraction in 1 of 10 in group CR, and in 6 of 8 in group NR (P &#60; 0.05, chi-square test). HCV RNA was positive in the bottom fraction of all samples. In a follow-up study of group NR, HCV RNA was detected in the top fraction in 3 of 8 just after IFN therapy, and in 7 of 8 after 1 year. This study suggests that the presence of HCV in the top fraction can predict a poor response to IFN therapy.</p>
キーワード IL-2R ??chain phorbol ester monocyte differentiation protein kinase
Amo Type Article
発行日 1996-06
出版物タイトル Acta Medica Okayama
50巻
3号
出版者 Okayama University Medical School
開始ページ 145
終了ページ 150
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8805853
Web of Sience KeyUT A1996UU60400005