このエントリーをはてなブックマークに追加
ID 17608
Eprint ID
17608
フルテキストURL
Thumnail 85_481.pdf 290 KB
タイトル(別表記)
Some Consideration on Cation Measurement of Bacterial Cell
著者
片山 健 岡山大学医学部微生物学教室
友近 健一 岡山大学医学部微生物学教室
高津 智子 岡山大学医学部微生物学教室
金政 泰弘 岡山大学医学部微生物学教室
俵 寿太郎 岡山大学医学部微生物学教室
抄録
The precise mensuration of the intracellular cation content is very significant from the biological point of view. When a salt concentration is medium is higher than in bacterial cells, it is very difficult to measure the intracellular cation content because of extracellular high contamination. In such a case, there is no other way except repeated washing for elimination of the outer contaminants. With the repeated washing method, authors attempted to measure accurately the intracellular cation content of Staph. aureus 209 P, cultivated in the medium of the various degrees of salt concentration, by flame photometory and atomic absorption spectrophotometory. The results were as follows. 1. When univalent cation concentration in cells is lower than in medium, it is necessary to wash the cells several times. The washing effect on extracellular contaminants could agree with the theoretical equation of washing that the contaminant decreased exponentially. The successive decrease of assay values by an easy grade must show a leakage of the intracellular Na. The reflective point could be considerated to show the Na content. 2. As in general potassium concentration in cells is higher than in medium, the accurate value was decided by extrapolation of the linear analytical quantity vs. washing time to zero time. 3. In spite of the extreme variation of sodium salt concentration in culture medium, the univalent cation content in Staph. aureus 209 P had hardly any shifts. 4. Bivalent cations, Mg and Ca, were not affected by the washing.
発行日
1973-10-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
85巻
9-10号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
481
終了ページ
486
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/85/9-10/85_9-10_481/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma