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ID 21178
Eprint ID
21178
フルテキストURL
タイトル(別表記)
Determination of copper and zinc in synovial fluid by atomic absorption spectrophotometry
著者
古野 勝志 岡山大学温泉研究所温泉内科学部門
抄録
Copper (Cu) and zinc (Zn) in synovial fluids of 52 patients with rheumatoid arthritis (RA) and of 53 patients with osteoarthritis (OA) were determined using a HITACHI MODEL 207 atomic absorption spectrophotometer. Standard solution of Cu and Zn were 50μg, 100μg, 200μg and 300μg per 100 ml 20 v/v % glycerine containing 0.01 N-HCl. To 0.5ml synovial fluid, 50 units of hyaluronidase was added and then the mixture were incubated for 20 minutes at 37℃. Synovial fluid treated with hyaluronidase and standard solution of Cu and Zn were diluted five fold with deionized distillation water, then aspirated directly into the burner. The relative absorbance was expressed as scale reading by a HITACHI MODEL QPD-54 recoder. The mean recovery rate of Cu and Zn in synovial fluids were 102.0% and 101.9%. By this method the concentrations of Cu and Zn in 52 RA (12 males, 40 females) were 97.6±29.0 (S.D.) μg/100 ml and 83.9±40.1 (S.D.) μg/100 ml ; 97.3±27.1 (S.D.)μg/100 ml, 78.7±29.9 (S.D.) μg /100 ml in 12males and 97.7±29.5 (S.D.) μg/100 ml, 85.5±42.6 (S.D.) μg /100 ml in 40 females respectively. The concentrations of Cu and Zn in 53 OA (14 males, 39 females) were 46.9±14.5 (S.D.) μg/100 ml and 40.8±14.3 (S.D.) μg/100 ml; 47.3±9.7 (S.D.) JLg/100 ml, 45.1±19.0 (S.D.) μg/ 100 ml in 14 males and 46.8±15.8 (S.D.) μg/100 ml, 39.3±11.8 (S.D.) μg/100 ml in 39 females respectively. No sex differences of Cu and Zn values were observed in both RA and OA. In patients with RA, concentrations of Cu and Zn in synovial fluids were signifcantly higher than in OA in both sexes. There were positive correlation between the concentrations of Cu and Zn in 105 patients (RA and OA, r=0.74, p<0.01) , in 52 RA (r=0.54, p<0.01), in 53 OA (r=0.81, p<0.01). In patients with RA, there were no correlation between stage and concentrations of Cu and Zn in synovial fluids.
備考
原著論文 (Original Papers)
発行日
1979-03-25
出版物タイトル
岡山大学温泉研究所報告
出版物タイトル(別表記)
Papers of the Institute for Thermal Spring Research, Okayama University
48巻
出版者
岡山大学温泉研究所
出版者(別表記)
Institute for Thermal Spring Research, Okayama University
開始ページ
13
終了ページ
23
ISSN
0369-7142
NCID
AN00032853
資料タイプ
紀要論文
言語
Japanese
OAI-PMH Set
岡山大学
論文のバージョン
publisher
査読
無し
Sort Key
5
Eprints Journal Name
mmc